Abstract: Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

Abstract: This invention relates to a surface finish manufacturing system and process and more particularly, but not exclusively, to a contrast surface finish manufacturing system and process for producing boards with a wood texture finish. The surface finish manufacturing system comprises a top layer and a bottom layer where part of the top layer is removed to expose part of the bottom layer.

Abstract: This invention relates to a deck board and more particularly, to a reduced material deck board. In accordance with this invention there is provided a deck board comprising an operatively upper board section having at least one operatively downwardly depending support leg terminating in at least one flange, an underside of the flange having a concave surface.

Abstract: Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

Abstract: Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

Abstract: Methods of preparing liquid exchanged biological fractions are provided. Aspects of the present disclosure according to certain embodiments include introducing a biological sample into a centrifugation container having a buoy, subjecting the biological sample to a force of centrifugation to produce two or more fractions in the biological sample, collecting a first fraction of the biological sample from the container and introducing a liquid exchange fluid into the container to produce a liquid exchanged fraction that is a mixture of the liquid exchange fluid and a second fraction of the biological sample. Compositions having a liquid exchanged platelet-rich fluid and kits for practicing embodiments of the subject methods are also provided. Methods for applying compositions having a liquid exchanged platelet-rich fluid to a body site of the subject are described.

Abstract: This invention relates to a deck board (1) and more particularly, to a reduced material deck board (1). In accordance with this invention there is provided a deck board (1) comprising an operatively upper board section (4) having at least one operatively downwardly depending support leg (5) terminating in at least one flange (7), an underside of the flange (7) having a concave surface.

Abstract: Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

Abstract: The present invention provides methods and reagents for directing the selective targeting and entry of bacterial toxins to mammalian cells. Methods include the step of contacting a cell with a bacterial toxin or toxic bioactive fragment thereof associated with an antibody or ligand that is specific to a target on the cell, wherein the antibody or ligand selectively binds the target on the cell and the bacterial toxin or fragment thereof is internalized and enters the cell. The invention further encompasses compositions and kits to carry out the methods.

Abstract: The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation.

Abstract: Aspects of the present disclosure include methods for preparing biocomposites having viable stem cells and platelets. Methods according to certain embodiments include combining a composition that contains viable stem cells with a composition the contains platelets in a centrifugation vessel, subjecting the centrifugation vessel to a force of centrifugation to produce two or more fractions such that each fraction includes a component having different density and collecting a fraction from the centrifuge vessel that includes the platelets and viable stem cells. Liquid biocomposites having viable stem cells and platelets and methods for administering the biocomposites to a body site of a subject are also provided.

Abstract: The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation.

Abstract: The present invention provides methods and reagents for directing the selective targeting and entry of bacterial toxins to mammalian cells. Methods include the step of contacting a cell with a bacterial toxin or toxic bioactive fragment thereof associated with an antibody or ligand that is specific to a target on the cell, wherein the antibody or ligand selectively binds the target on the cell and the bacterial toxin or fragment thereof is internalized and enters the cell. The invention further encompasses compositions and kits to carry out the methods.

Abstract: It is disclosed here that synaptotagmin I (syt I) and synaptotagmin II (syt II) are the cellular receptors for botulinum neurotoxin B (BoNT/B) that mediate the cellular entry and toxicity of BoNT/B. The BoNT/B binding domains of syt I and II are also disclosed. While syt I needs gangliosides for BoNT/B binding, syt II can bind to BoNT/B in the absence of gangliosides. Various nucleic acids and polypeptides that relate to the BoNT/B binding domain of syt I or II are disclosed. Further disclosed are methods of reducing BoNT/B toxicity, methods of identifying agents that can block the binding between BoNT/B and syt I or II, methods of identifying agents that can bind to the BoNT/B binding domain of syt I or II, methods of detecting BoNT/B or Clostridium botulinum and kits for use thereof.

Abstract: A mobile wireless communications device may include a housing having an opening therein and an inwardly extending housing ring adjacent the opening, and a switch carried by the housing and being aligned with the opening. The mobile wireless communications device may further include a backing member within the housing adjacent the opening, and an elastomeric switch membrane comprising a body covering the switch, and a flange coupled to the body and compressed between the backing member and the housing ring to define a seal therewith. The flange may include a flange base, and a pair of flange rings extending inwardly from the flange base and defining a valley therebetween aligned with the housing ring. In another embodiment, the flange may include a flange base and a first flange ring on a first surface thereof, and a pair of second flange rings on a second surface of the flange base and defining a valley therebetween aligned with the first flange ring.

Abstract: It is disclosed here that synaptotagmin I (syt I) and synaptotagmin II (syt II) are the cellular receptors for botulinum neurotoxin B (BoNT/B) that mediate the cellular entry and toxicity of BoNT/B. The BoNT/B binding domains of syt I and II are also disclosed. While syt I needs gangliosides for BoNT/B binding, syt II can bind to BoNT/B in the absence of gangliosides. Various nucleic acids and polypeptides that relate to the BoNT/B binding domain of syt I or II are disclosed. Further disclosed are methods of reducing BoNT/B toxicity, methods of identifying agents that can block the binding between BoNT/B and syt I or II, methods of identifying agents that can bind to the BoNT/B binding domain of syt I or II, methods of detecting BoNT/B or Clostridium botulinum and kits for use thereof.

Abstract: A mobile wireless communications device may include a housing having an opening therein and an inwardly extending housing ring adjacent the opening, and a switch carried by the housing and being aligned with the opening. The mobile wireless communications device may further include a backing member within the housing adjacent the opening, and an elastomeric switch membrane comprising a body covering the switch, and a flange coupled to the body and compressed between the backing member and the housing ring to define a seal therewith. The flange may include a flange base, and a pair of flange rings extending inwardly from the flange base and defining a valley therebetween aligned with the housing ring. In another embodiment, the flange may include a flange base and a first flange ring on a first surface thereof, and a pair of second flange rings on a second surface of the flange base and defining a valley therebetween aligned with the first flange ring.

Abstract: A tool insert comprises a substrate (40), a layer of ultra-hard abrasive material (52) bonded to the substrate and presenting a primary cutting edge (54) for the tool insert, and a protective layer (56) bonded to the ultra-hard abrasive material so as to protect the primary cutting edge thereof. The protective layer provides a secondary cutting edge (58) for the tool insert, the depth of the protective layer being selected so as to be sufficient to protect the primary cutting edge whilst cutting, milling or drilling a window through a first substance, such as a casing or lining of a borehole or shaft, but to expose the primary cutting edge upon encountering a second substance, such as a rockbed. The invention extends to a method of drilling a horizontal or angled hole in a sub-terranean rock formation using a rotary drill string and bit incorporating a tool insert of the invention.

Abstract: A composite material comprises a plurality of cores of material selected from the group comprising carbides, nitrides, carbonitrides, cemented carbides, cemented nitrides, cemented carbonitrides and mixtures thereof, dispersed in a matrix. The matrix comprises the components for making an ultra-hard material, such as diamond or cBN abrasive particles, and a suitable binder. The ultra-hard material is polycrystalline in nature and is typically PCD or PcBN. The cores are typically provided as individual particles or in the form of granules. The granules may be further coated with a second coating, which may be a similar material to that of the cores or of an ultra-hard material of a different grade to that of the first coating. The composite material typically takes on a honeycomb structure of a hard material and cores within the pores of the honeycomb structure bonded to the honeycomb structure. The pores of the honeycomb structure may be ordered or random.

Abstract: A tool insert comprises a substrate (10) having a support surface and a support ring extending laterally from the support surface. The support ring is sized to define a recess (14) within the confines thereof and a shelf about the periphery thereof. A layer (12) of ultra-hard abrasive material is located within the recess and bonded to the substrate and the support ring (16), and presents a primary cutting edge (22) for the tool insert. A protective layer (18) is bonded to the shelf about the support ring so as to protect the primary cutting edge. The protective layer provides a secondary cutting edge (20) for the tool insert, the depth of the protective layer being selected so as to be sufficient to protect the primary cutting edge whilst cutting, milling or drilling through a first substance but to expose the primary cutting edge upon encountering a second substance.