Abstract: Natural product preparations of isoquinoline alkaloids are obtained from plant callus cells of goldenseal, Hydrastis canadensis, grown in suspension cell culture. The natural product preparations can be extracted from the cells and/or obtained from the culture medium in which the cells are grown. Accordingly, callus cell lines can be selected to grow in suspension culture, produce desirable levels and/or ratios of isoquinoline alkaloids, and/or release the isoquinoline alkaloids into the liquid medium. The level and/or ratio of certain isoquinoline alkaloids, such as hydrastine and/or berberine produced by the cells and/or released into the medium can be different than those found in native plants or plant parts (e.g., rhizomes) from which the cells were derived. For instance, cell lines can be selected to increase the production and/or ratio of hydrastine to berberine, or vice versa.

Abstract: A dermatological composition for inhibiting or treating inflammation and/or aging of skin includes a dermatologically compatible component and cultured cells derived from the Theobroma cacao plant or an extract thereof. The cultured cells including anti-oxidant and/or anti-inflammatory compounds that reduce inflammation and/or aging when delivered to the skin.

Abstract: A method for producing metabolites of Crocus sativus (C. sativus) includes (i) selecting a cell line of C. sativus that produces one or more saffron metabolites in cell suspension culture, and (ii) growing the selected cell line in a suspension cell culture to produce the saffron metabolite.