Abstract: Described herein are functional cell assays and methods for selecting a personalized anti-cancer treatment regimen that can improve treatment of cancer in a subject, identify resistance of the subject's cancer to one or more anti-cancer agents and/or validate the current drug treatment strategy.

Abstract: Compositions, targeting reagents, modified cells, nucleic acid molecules, systems, and methods for identifying and selecting genomic safe harbor sites for transgene insertion and other genome engineering applications. These materials and methods can be used to develop desired genome engineering applications, such as transgene insertion and expression or genome modification, that take into account the application-specific needs for safety, functional silence, and accessibility and other factors that vary with a desired application's goals and target population. Representative examples of desired genome engineering applications include, but are not limited to, transgene insertion, such as therapeutic transgene insertion, functional gene editing, gene or chromosomal location-specific structural modification, cell marking, gene activation, and/or gene repression.