Abstract: An electrophoresis device is disclosed which may be partially disassembled and sterilized in an autoclave. The device includes a base and support framework extending from the base. A separation column including a separation chamber having a pair of plates spaced from and positioned generally parallel to one another is supported from the framework. A pump is provided for pumping buffer solution through the separation chamber. A sample injection device is located near an inlet of the separation chamber and a collection assembly is located near the outlet of the separation chamber. An electrical compartment is supported by the framework and controls the pump, injection device and collection assembly and is removably located in the electrical compartment. The separation column plates are made of a heat resistant plastic.