Patents by Inventor Raymond L. Rodriguez

Raymond L. Rodriguez has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6284956
    Abstract: A plant transformation expression cassette for transforming host plant cells with a selectable marker gene is disclosed. The cassette includes, operatively linked in sequence in a 5′ to 3′ direction, (i) a DNA promoter sequence from the rice beta-glucanase 9 (gns9) gene; (ii) a selectable marker gene, and (iii) a 3′ untranslated terminator region. Also disclosed are a expression cassette pair containing the transformation vector, a method of obtaining transformed monocots whose seeds produce a selected heterologous protein during sed germination, and a plant whose cells are transformed with the chimeric selectable marker gene.
    Type: Grant
    Filed: June 25, 1999
    Date of Patent: September 4, 2001
    Assignee: Applied Phytologics, Inc.
    Inventors: Raymond L Rodriguez, Ning Huang
  • Patent number: 6127145
    Abstract: A method for producing .alpha..sub.1 -antitrypsin (AAT) in plant cells is disclosed. Monocot plant cells transformed with an AAT coding sequence are cultivated under conditions efficient for protein expression and secretion. Also disclosed are a codon-optimized AAT coding sequence that is efficiently translated in plant cell culture and a novel AAT protein having the glycosylation pattern characteristic of plant cells and suitable for therapeutic use in humans.
    Type: Grant
    Filed: February 13, 1998
    Date of Patent: October 3, 2000
    Assignee: Applied Phytologics, Inc.
    Inventors: Thomas D. Sutliff, Raymond L. Rodriguez
  • Patent number: 6100447
    Abstract: A method for stably transforming barley from mature barley seeds as starting material is disclosed. The method involves germinating mature barley seeds until early shoot development occurs, exposing scutellar or embryo tissue cells on the embryo side of germinated seeds, and introducing foreign DNA into the cells. The cells are initially grown under conditions that allow expression of a selectable marker introduced with the foreign DNA, then on a callus-growth medium effective to suppress callus formation in the absence of the selectable marker. Successfully transformed calli can be cultured in suspension to obtain a desired foreign protein, or regenerated into plants, to obtain the foreign protein from the transformed plants, e.g., germinated seeds.
    Type: Grant
    Filed: February 12, 1998
    Date of Patent: August 8, 2000
    Assignee: Applied Phytologics, Inc.
    Inventors: Liying Wu, Raymond L. Rodriguez
  • Patent number: 6066781
    Abstract: A chimeric gene for use in producing a mature protein in secreted form by stably transformed plant cells is disclosed. The gene includes a DNA coding sequence encoding a fusion protein having an (i) N-terminal moiety corresponding to the portion of the rice .alpha.-amylase signal sequence peptide identified by SEQ ID: 1 and, (ii) immediately adjacent the C-terminal amino acid of said portion, a protein moiety corresponding to the protein to be produced. Also disclosed are a fusion protein encoded by the gene, and a method of producing a mature protein in secreted form by plant cells.
    Type: Grant
    Filed: February 13, 1998
    Date of Patent: May 23, 2000
    Assignee: Applied Phytologics, Inc.
    Inventors: Thomas D. Sutliff, Raymond L. Rodriguez
  • Patent number: 6048973
    Abstract: A chimeric plant promoter which is responsive to sugar depletion or deprivation is disclosed. The promoter includes a promoter element and, carried in the element, a heterologous sequence from the rice Amy3D that is responsive to sugar depletion. Also disclosed is a plant transfecting vector containing the sequence, and plant cell transfected with the vector.
    Type: Grant
    Filed: March 24, 1998
    Date of Patent: April 11, 2000
    Assignee: The Regents of the University of California
    Inventor: Raymond L. Rodriguez
  • Patent number: 5994628
    Abstract: This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins by malting of monocot plant seeds. The heterologous genes are expressed during germination of the seeds and isolated from a malt. Also disclosed are chimeric genes, vectors and methods relating to the present invention. Protein production by cell culture techniques is also described.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: November 30, 1999
    Assignee: The Regents of the University of California
    Inventor: Raymond L. Rodriguez
  • Patent number: 5888789
    Abstract: This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins by malting of monocot plant seeds. The heterologous genes are expressed during germination of the seeds and isolated from a malt. Also disclosed are chimeric genes, vectors and methods relating to the present invention. Protein production by cell culture techniques is also described.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: March 30, 1999
    Assignee: The Regents of the University of California
    Inventor: Raymond L. Rodriguez
  • Patent number: 5889189
    Abstract: This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins by malting of monocot plant seeds. The heterologous genes are expressed during germination of the seeds and isolated from a malt. Also disclosed are chimeric genes, vectors and methods relating to the present invention. Protein production by cell culture techniques is also described.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: March 30, 1999
    Assignee: The Regents of the University of California
    Inventor: Raymond L. Rodriguez
  • Patent number: 5693506
    Abstract: This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins in plant cultu1res and seeds. Where seeds are the source of the protein, the heterologous genes are expressed during germination and isolated from a malt.
    Type: Grant
    Filed: November 16, 1993
    Date of Patent: December 2, 1997
    Assignee: The Regents of the University of California
    Inventor: Raymond L. Rodriguez
  • Patent number: 4588689
    Abstract: A novel restriction endonuclease designated XcyI recognizes and cleaves the sequence 5'-C.dwnarw.CCGGG-3', where the arrow indicates the cleavage site. The enzyme may be obtained from Xanthamonas cyanopsidis.Xanthamonas cyanopsidis strain 13D5 was deposited at the American Type Culture Collection on Jan. 20, 1984, and granted accession No. 39587.
    Type: Grant
    Filed: March 2, 1984
    Date of Patent: May 13, 1986
    Assignee: The Regents of the University of California
    Inventors: Clarence I. Kado, Raymond L. Rodriguez, Byron E. Froman, Robert C. Tait