Patents by Inventor Raymond P. Mariella, Jr.
Raymond P. Mariella, Jr. has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20130306481Abstract: A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.Type: ApplicationFiled: July 18, 2013Publication date: November 21, 2013Applicant: Lawrence Livermore National Security, LLCInventors: Dietrich A. Dehlinger, Klint A. Rose, Maxim Shusteff, Christopher G. Bailey, Raymond P. Mariella, JR.
-
Patent number: 8524064Abstract: A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.Type: GrantFiled: July 26, 2010Date of Patent: September 3, 2013Assignee: Lawrence Livermore National Security, LLCInventors: Dietrich A. Dehlinger, Klint A. Rose, Maxim Shusteff, Christopher G. Bailey, Raymond P. Mariella, Jr.
-
Patent number: 8470537Abstract: A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.Type: GrantFiled: December 3, 2010Date of Patent: June 25, 2013Assignee: Lawrence Livermore National Security, LLCInventors: Shea N. Gardner, Raymond P. Mariella, Jr., Allen T. Christian, Jennifer A. Young, David S. Clague
-
Publication number: 20130043170Abstract: An ultrasonic microfluidic system includes a separation channel for conveying a sample fluid containing small particles and large particles, flowing substantially parallel, adjacent to a recovery fluid, with which it is in contact. An acoustic transducer produces an ultrasound standing wave, that generates a pressure field having at least one node of minimum pressure amplitude. An acoustic extension structure is located proximate to said separation channel for positioning said acoustic node off center in said acoustic area and concentrating the large particles in said recovery fluid stream.Type: ApplicationFiled: August 10, 2012Publication date: February 21, 2013Inventors: Klint A. Rose, Karl A. Fisher, Douglas A. Wajda, Raymond P. Mariella, JR., Christopher Bailey, Dietrich Dehlinger, Maxim Shusteff, Byoungsok Jung, Kevin D. Ness
-
Publication number: 20120175258Abstract: An isotachophoresis system for separating a sample containing particles into discrete packets including a flow channel, the flow channel having a large diameter section and a small diameter section; a negative electrode operably connected to the flow channel; a positive electrode operably connected to the flow channel; a leading carrier fluid in the flow channel; a trailing carrier fluid in the flow channel; and a control for separating the particles in the sample into discrete packets using the leading carrier fluid, the trailing carrier fluid, the large diameter section, and the small diameter section.Type: ApplicationFiled: May 12, 2011Publication date: July 12, 2012Applicant: Lawrence Livermore National Security, LLCInventor: Raymond P. Mariella, JR.
-
Publication number: 20110259745Abstract: A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.Type: ApplicationFiled: July 26, 2010Publication date: October 27, 2011Inventors: Dietrich A. Dehlinger, Klint A. Rose, Maxim Shusteff, Christopher G. Bailey, Raymond P. Mariella, JR.
-
Publication number: 20110124098Abstract: A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter.Type: ApplicationFiled: November 10, 2010Publication date: May 26, 2011Inventors: Klint A. Rose, Raymond P. Mariella, JR., Christopher G. Bailey, Kevin Dean Ness
-
Publication number: 20110104763Abstract: A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.Type: ApplicationFiled: December 3, 2010Publication date: May 5, 2011Inventors: Shea N. Gardner, Raymond P. Mariella, JR., Allen T. Christian, Jennifer A. Young, David S. Clague
-
Patent number: 7871799Abstract: A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.Type: GrantFiled: December 3, 2003Date of Patent: January 18, 2011Assignee: Lawrence Livermore National Security, LLCInventors: Shea N. Gardner, Raymond P. Mariella, Jr., Allen T. Christian, Jennifer A. Young, David S. Clague
-
Publication number: 20090194420Abstract: Systems and methods for separating particles and/or toxins from a sample fluid. A method according to one embodiment comprises simultaneously passing a sample fluid and a buffer fluid through a chamber such that a fluidic interface is formed between the sample fluid and the buffer fluid as the fluids pass through the chambers the sample fluid having particles of interest therein; applying a force to the fluids for urging the particles of interest to pass through the interface into the buffer fluid; and substantially separating the buffer fluid from the sample fluid.Type: ApplicationFiled: February 1, 2008Publication date: August 6, 2009Inventors: Raymond P. Mariella, JR., George M. Dougherty, John M. Dzenitis, Robin R. Miles, David S. Clague
-
Patent number: 7452666Abstract: A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.Type: GrantFiled: March 21, 2003Date of Patent: November 18, 2008Assignee: Lawrence Livermore National Security, LLCInventor: Raymond P. Mariella, Jr.
-
Patent number: 7090979Abstract: A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.Type: GrantFiled: November 21, 2003Date of Patent: August 15, 2006Assignee: The Regents of the University of CaliforniaInventors: Raymond P. Mariella, Jr., Allen T. Christian, James D. Tucker, John M. Dzenitis, Alexandros P. Papavasiliou
-
Patent number: 6866759Abstract: A fluidic channel patterned with a series of thin-film electrodes makes it possible to move and concentrate DNA in a fluid passing through the fluidic channel. The DNA has an inherent negative charge and by applying a voltage between adjacent electrodes the DNA is caused to move. By using a series of electrodes, when one electrode voltage or charge is made negative with respect to adjacent electrodes, the DNA is repelled away from this electrode and attached to a positive charged electrode of the series. By sequentially making the next electrode of the series negative, the DNA can be moved to and concentrated over the remaining positive electrodes.Type: GrantFiled: December 13, 2000Date of Patent: March 15, 2005Assignee: The Regents of the University of CaliforniaInventors: Robin R. Miles, Amy Wei-Yun Wang, Raymond P. Mariella, Jr.
-
Patent number: 6787104Abstract: A system for detection and treatment of chemical weapons and/or biological pathogens uses a detector system, an electrostatic precipitator or scrubber, a circulation system, and a control. The precipitator or scrubber is activated in response to a signal from the detector upon the detection of chemical weapons and/or biological pathogens.Type: GrantFiled: September 14, 2000Date of Patent: September 7, 2004Assignee: The Regents of the University of CaliforniaInventor: Raymond P. Mariella, Jr.
-
Patent number: 6761811Abstract: A system utilizing multi-stage traps based on dielectrophoresis. Traps with electrodes arranged transverse to the flow and traps with electrodes arranged parallel to the flow with combinations of direct current and alternating voltage are used to trap, concentrate, separate, and/or purify target particles.Type: GrantFiled: March 27, 2001Date of Patent: July 13, 2004Assignee: The Regents of the University of CaliforniaInventor: Raymond P. Mariella, Jr.
-
Patent number: 6730204Abstract: An apparatus is adapted to separate target materials from other materials in a flow containing the target materials and other materials. A dielectrophoretic trap is adapted to receive the target materials and the other materials. At least one electrode system is provided in the trap. The electrode system has a three-dimensional configuration. The electrode system includes a first electrode and a second electrode that are shaped and positioned relative to each such that application of an electrical voltage to the first electrode and the second electrode creates a dielectrophoretic force and said dielectrophoretic force does not reach zero between the first electrode and the second electrode.Type: GrantFiled: March 27, 2001Date of Patent: May 4, 2004Assignee: The Regents of the University of CaliforniaInventor: Raymond P. Mariella, Jr.
-
Patent number: 6154276Abstract: A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.Type: GrantFiled: February 23, 1998Date of Patent: November 28, 2000Assignee: The Regents of the University of CaliforniaInventor: Raymond P. Mariella, Jr.
-
Patent number: 5589136Abstract: A silicon-based sleeve type chemical reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The reaction chamber combines a critical ratio of silicon and silicon nitride to the volume of material to be heated (e.g., a liquid) in order to provide uniform heating, yet low power requirements. The reaction chamber will also allow the introduction of a secondary tube (e.g., plastic) into the reaction sleeve that contains the reaction mixture thereby alleviating any potential materials incompatibility issues. The reaction chamber may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The reaction chamber may also be used in synthesis instruments, particularly those for DNA amplification and synthesis.Type: GrantFiled: June 20, 1995Date of Patent: December 31, 1996Assignee: Regents of the University of CaliforniaInventors: M. Allen Northrup, Raymond P. Mariella, Jr., Anthony V. Carrano, Joseph W. Balch
-
Patent number: 5475487Abstract: The liquid of a flow cytometer itself acts as an optical waveguide, thus transmitting the light to an optical filter/detector combination. This alternative apparatus and method for detecting scattered light in a flow cytometer is provided by a device which views and detects the light trapped within the optical waveguide formed by the flow stream. A fiber optic or other light collecting device is positioned within the flow stream. This provides enormous advantages over the standard light collection technique which uses a microscope objective. The signal-to-noise ratio is greatly increased over that for right-angle-scattered light collected by a microscope objective, and the alignment requirements are simplified.Type: GrantFiled: April 20, 1994Date of Patent: December 12, 1995Assignee: The Regents of the University of CaliforniaInventors: Raymond P. Mariella, Jr., Gerrit van den Engh, M. Allen Northrup
-
Patent number: 5404026Abstract: A single-crystal, multi-layer device incorporating an IR absorbing layer that is compositionally different from the Ga.sub.x Al.sub.1-x Sb layer which acts as the electron emitter. Many different IR absorbing layers can be envisioned for use in this embodiment, limited only by the ability to grow quality material on a chosen substrate. A non-exclusive list of possible IR absorbing layers would include GaSb, InAs and InAs/Ga.sub.w In.sub.y Al.sub.1-y-w Sb superlattices. The absorption of the IR photon excites an electron into the conduction band of the IR absorber. An externally applied electric field then transports electrons from the conduction band of the absorber into the conduction band of the Ga.sub.x Al.sub.1-x Sb, from which they are ejected into vacuum. Because the band alignments of Ga.sub.x Al.sub.1-x Sb can be made the same as that of GaAs, emitting efficiencies comparable to GaAs photocathodes are obtainable.Type: GrantFiled: January 14, 1993Date of Patent: April 4, 1995Assignee: Regents of the University of CaliforniaInventors: Raymond P. Mariella, Jr., Gregory A. Cooper