Abstract: A multi-spectral image system and method for cytology. The multi-spectral imaging system comprises an optical stage, an image capture camera, and a controller. The optical stage includes a light source for illuminating the cytological specimen and optical means for producing images of the illuminated specimen in a number of spectral bands. The image capture camera includes means for simultaneously capturing the spectral images and generating electrical signals corresponding to the captured images. The controller controls the operation of the image capture camera and the light source and includes means for converting the electrical signals into a data form suitable for further processing. The multi-spectral imaging system is particularly suited for specimens prepared in the form of thin-layers or monolayers. The image data produced by the relevant state of the specimen and also permits the use of human-expert review for confirmation.