Abstract: Memory-like cytokine enhanced NK (M-CENK) cells have superior cytotoxicity and can be generated/expanded from a single batch of mononuclear cells to in sufficient quantities to so form a cell-based therapeutic suitable for infusion. Advantageously, the M-CENK cells can be cryopreserved and thawed without compromising viability and cytotoxicity.

Abstract: Memory-like cytokine enhanced NK (M-CENK) cells have superior cytotoxicity and can be generated/expanded from a single batch of mononuclear cells to in sufficient quantities to so form a cell-based therapeutic suitable for infusion. Advantageously, the M-CENK cells can be cryopreserved and thawed without compromising viability and cytotoxicity.

Abstract: Cells and cell-based therapeutic compositions and methods are presented in which the cells are CD16+CD57+NK-92MI cells that natively express CD16 and CD57 and that exhibit IL-2 independent growth.

Abstract: Provided herein are pharmaceutical compositions, comprising a pharmaceutically acceptable carrier and therapeutically effective amounts of high affinity Natural Killer (haNK) cells and a therapeutic antibody in the form of a combined preparation. Also provided herein are methods for treating cancer by using the pharmaceutical composition comprising the haNK cells and the therapeutic antibody.

Abstract: Provided herein are methods of culturing NK-92® cells using the growth media containing a non-ionic surfactant such that the cell culture have reduced clumping as compared to control NK-92® cells that have been cultures in a control medium lacking the non-ionic surfactant. The growth medium comprises 0.025 to 0.9% of a non-ionic surfactant, e.g., Poloxamer 188.

Abstract: Provided herein are methods and medium compositions for cryopreserving NK-92® cells.

Abstract: Provided herein are methods of harvesting NK-92® cells comprising collecting NK-92® cells from a cell culture and washing the collected NK-92® cells in a buffer comprising 1%-5% albumin.

Abstract: Methods and systems for coordinating delivery of coupons issued by a network-based system are disclosed. For example, a system can include a coupon generation engine, a coupon widget, and a communications module. The coupon generation engine can issue a unique coupon redeemable exclusively by a registered user of the network-based system. The coupon widget can present the unique coupon via a first communication channel internal to the network-based system. The communications module can deliver the unique coupon to the registered user via a second communication channel external to the network-based system.

Abstract: Methods and systems for issuing targeted coupons with a network-based system is disclosed. For example, a system can include a targeting module, a coupon rules engine, a coupon generation engine, a coupon widget, and a checkout module. The targeting module can create a model for controlling coupon generation. The coupon rules engine can determine whether a user accessing the network-based system qualifies to receive a coupon based at least in part on application of the model created by the targeting module. The coupon generation engine can generate a coupon based on the coupon rules engine determining that the user qualifies to receive a coupon. The coupon widget can present the coupon to the user while the user is accessing the network-based system. Finally, the checkout module can apply the coupon to a purchase by the user.

Abstract: A device that includes a frame having a first part and a second part and a plurality of first suction devices connected to the first part of the frame, the first suction devices positioned so as to contact a glass plane. Also included in the device is a plurality of second suction devices connected to the second part of the frame, the second suction devices positioned so as to contact a body having an opening to receive the glass plane. A plurality of positioning components that position the frame with respect to the opening in the body, the positioning components connected to the first part of the frame and a plurality of shimming elements that move down between the glass plane and the opening and shift the glass plane forward, the shimming elements connected to the first part of the frame are as included in the device.

Abstract: A device that includes a frame having a first part and a second part and a plurality of first suction devices connected to the first part of the frame, the first suction devices positioned so as to contact a glass plane. Also included in the device is a plurality of second suction devices connected to the second part of the frame, the second suction devices positioned so as to contact a body having an opening to receive the glass plane. A plurality of positioning components that position the frame with respect to the opening in the body, the positioning components connected to the first part of the frame and a plurality of shimming elements that move down between the glass plane and the opening and shift the glass plane forward, the shimming elements connected to the first part of the frame are as included in the device.

Abstract: Methods and systems for coordinating delivery of coupons issued by a network-based system are disclosed. For example, a system can include a coupon generation engine, a coupon widget, and a communications module. The coupon generation engine can issue a unique coupon redeemable exclusively by a registered user of the network-based system. The coupon widget can present the unique coupon via a first communication channel internal to the network-based system. The communications module can deliver the unique coupon to the registered user via a second communication channel external to the network-based system.

Abstract: Methods and systems for displaying coupons within a network-based system are disclosed. For example, a system can include a database, a monitoring module, a coupon rules engine, and a coupon widget. The database to store account information associated with a user, the account information identifying one or more unique coupons redeemable exclusively by the user. The monitoring module to monitor interactions by the user with the network-based system. The coupon rules engine to select a selected coupon, of the one or more unique coupons redeemable exclusively by the user, that is eligible to be presented to the user based on the monitored interactions by the user with the network-based system. The coupon widget to present the selected coupon to the user.

Abstract: Methods and systems for issuing targeted coupons with a network-based system is disclosed. For example, a system can include a targeting module, a coupon rules engine, a coupon generation engine, a coupon widget, and a checkout module. The targeting module can create a model for controlling coupon generation. The coupon rules engine can determine whether a user accessing the network-based system qualifies to receive a coupon based at least in part on application of the model created by the targeting module. The coupon generation engine can generate a coupon based on the coupon rules engine determining that the user qualifies to receive a coupon. The coupon widget can present the coupon to the user while the user is accessing the network-based system. Finally, the checkout module can apply the coupon to a purchase by the user.