Patents by Inventor Rebecca Anne Grace De Souza

Rebecca Anne Grace De Souza has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions for transfecting resistant cell types
    Patent number: 11679159
    Abstract: A reagent composition for delivery nucleic acid therapeutics to cells is provided. The reagent includes ionizable lipid, structural lipid, and a stabilizing agent which improves the transfection efficiency of nucleic acids, and leaves transfected cells viable. The 5 transfection reagent is effective in plasmid and mRNA delivery, and in neurons and progenitor-like cells. It may be used for ex vivo cell therapy.
    Type: Grant
    Filed: April 15, 2019
    Date of Patent: June 20, 2023
    Assignee: Precision Nanosystems ULC
    Inventors: Anitha Thomas, Andrew William Brown, Rebecca Anne Grace De Souza, Tara L. Fernandez
  • Compositions for transfecting resistant cell types
    Patent number: 11572575
    Abstract: A transfection reagent composition comprising: 30-60 MOL. % of a cationic lipid, or a pharmaceutical acceptable salt thereof; 10-60 MOL % of a structural lipid; 10-20 MOL % of a triglyceride; and 0.1 to about 10 MOL. % of a stabilizing agent, is provided. The transfection agent is effective in transfecting cells, particularly neurons, with siRNA, mRNA and plasmid nucleic acid, Sand maintaining viability of the cells as well as activity of the delivered nucleic acid.
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: February 7, 2023
    Assignee: Precision NanoSystems ULC
    Inventors: Anitha Thomas, Rebecca Anne Grace De Souza, Eric Ouellet, Grace Tharmini Tharmarajah, Jagbir Singh, Shyam Madhusudan Garg
  • Nonviral Modification of T Cell Gene Expression
    Publication number: 20220162552
    Abstract: There is provided a lipid mix composition comprising ionizable lipid, a structural lipid such as DSPC, a sterol, and a surfactant such as polysorbate 80, polyoxyethylene (10) stearyl ether, polyoxyethylene (20) stearyl ether, or D-?-Tocopherol polyethylene glycol 1000 succinate. The lipid mix compositions find particular use in transfecting difficult to transfect cells and maintaining the viability of those cells. The lipid mix compositions are particularly well suited to T cell transfection ex vivo.
    Type: Application
    Filed: April 14, 2020
    Publication date: May 26, 2022
    Applicant: Precision NanoSystems Inc.
    Inventors: Anitha Thomas, Andrew William Brown, Rebecca Anne Grace De Souza, Tara Fernandez
  • Compositions for Transfecting Resistant Cell Types
    Publication number: 20210284998
    Abstract: A transfection reagent composition comprising: 30-60 MOL % of an cationic lipid, or pharmaceutical acceptable salt thereof; 10-60 MOL % structural lipid; a sterol and 0.1 to about 10 MOL % of a stabilizing agent is provided. The reagent is particularly adapted for neuron and related cell types. A method of manufacturing LNP including nucleic acid for selective uptake into either neurons or astrocytes or neural progenitor cells is also provided.
    Type: Application
    Filed: October 2, 2017
    Publication date: September 16, 2021
    Applicant: Precision NanoSystems Inc.
    Inventors: Anitha Thomas, Rebecca Anne Grace De Souza, Eric Ouellet, Grace Tharmini Tharmarajah, Jagbir Singh, Shyam Madhusudan Garg
  • Compositions for Transfecting Resistant Cell Types
    Publication number: 20210162052
    Abstract: A reagent composition for delivery nucleic acid therapeutics to cells is provided. The reagent includes ionizable lipid, structural lipid, and a stabilizing agent which improves the transfection efficiency of nucleic acids, and leaves transfected cells viable. The 5 transfection reagent is effective in plasmid and mRNA delivery, and in neurons and progenitor-like cells. It may be used for ex vivo cell therapy.
    Type: Application
    Filed: April 15, 2019
    Publication date: June 3, 2021
    Applicant: Precision NanoSystems Inc.
    Inventors: Anitha Thomas, Andrew William Brown, Rebecca Anne Grace De Souza, Tara L. Fernandez
  • Compositions for Transfecting Resistant Cell Types
    Publication number: 20190292566
    Abstract: A transfection reagent composition comprising: 30-60 MOL. % of a cationic lipid, or a pharmaceutical acceptable salt thereof; 10-60 MOL % of a structural lipid; 10-20 MOL % of a triglyceride; and 0.1 to about 10 MOL. % of a stabilizing agent, is provided. The transfection agent is effective in transfecting cells, particularly neurons, with siRNA, mRNA and plasmid nucleic acid, Sand maintaining viability of the cells as well as activity of the delivered nucleic acid.
    Type: Application
    Filed: December 19, 2017
    Publication date: September 26, 2019
    Applicant: Precision NanoSystems Inc.
    Inventors: Anitha Thomas, Rebecca Anne Grace De Souza, Eric Ouellet, Grace Tharmini Tharmarajah, Jagbir Singh, Shyam Madhusudan Garg