Patents by Inventor Rebecca B. Kucera

Rebecca B. Kucera has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for cloning and producing the Msel restriction endonuclease
    Patent number: 6846658
    Abstract: A method for cloning restriction-modification system is provided whereby the target modification methylase is produced and confers full protection during all growth phases in which the cognate restriction enzyme is present. The method is employed in the cloning of the MseI restriction-modification system.
    Type: Grant
    Filed: October 12, 2000
    Date of Patent: January 25, 2005
    Assignee: New England Biolabs, Inc.
    Inventors: Romualdas Vaisvila, Richard D. Morgan, Rebecca B. Kucera, Toby E. Claus, Elisabeth A. Raleigh
  • Method for cloning and expression of OkrAI restriction endonuclease and methyltransferase
    Publication number: 20040175816
    Abstract: The present invention relates to recombinant DNA encoding the OkrAI restriction endonuclease as well as OkrAI methylase, expression of OkrAI restriction endonuclease in E. coli cells containing the recombinant DNA.
    Type: Application
    Filed: March 3, 2003
    Publication date: September 9, 2004
    Applicant: New England Biolabs, Inc.
    Inventors: Shuang-Yong Xu, Jian-Ping Xiao, Rebecca B. Kucera, James Samuelson
  • N. bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification
    Publication number: 20030211506
    Abstract: The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI and other nicking enzymes in non-modified strand displacement amplification, is disclosed also.
    Type: Application
    Filed: November 14, 2002
    Publication date: November 13, 2003
    Inventors: Huimin Kong, Lauren Sears Higgins, Michael A Dalton, Rebecca B Kucera, Ira Schildkraut, Geoffrey G Wilson
  • Cloning and producing the N.BstNBI nicking endonuclease
    Patent number: 6191267
    Abstract: The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI in non-thio strand displacement amplification, is disclosed also.
    Type: Grant
    Filed: June 2, 2000
    Date of Patent: February 20, 2001
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Lauren S. Higgins, Michael Dalton, Rebecca B. Kucera, Ira Schildkraut
  • Purified thermostable DNA polymerase obtainable from thermococcus litoralis
    Patent number: 5210036
    Abstract: There is provided an extremely thermostable enzyme obtainable from Thermococcus litoralis. The thermostable enzyme has a molecular weight of about 90,000-95,000 daltons, a half-life of about 60 minutes at 100.degree. C. in the absence of stabilizer, and a half-life of about 95 minutes at 100.degree. C. in the presence of stabilizer, such as octoxynol (TRITON X-100) or bovine serum albumin. The thermostable enzyme possesses a 3'-5' proofreading exonuclease activity. The thermostable enzyme may be native or recombinant and may be used for second-strand cDNA synthesis in cDNA cloning, DNA sequencing, and DNA amplification.
    Type: Grant
    Filed: April 26, 1990
    Date of Patent: May 11, 1993
    Assignee: New England Biolabs, Inc.
    Inventors: Donald G. Comb, Rebecca B. Kucera