Abstract: The systems and methods of the present disclosure provide techniques for the design and use of an intermediate or transitional plate or block designed to couple acoustic energy at a given frequency from a transducer, such as a piezoelectric transducer, to one or more acoustophoretic devices, such as microfluidic channels, such that driving the chip occurs with a controlled wavelength and symmetry. Such techniques provide improved efficiency when driving a single acoustophoretic device, or for multiple acoustophoretic devices to be operated in concert from a single transducer, and therefore without complex electronics. Additionally, the techniques described herein allow for relaxed design constraints when considering transducer selection and fabrication, instead transferring design constraints to the more easily customized actuation plate.

Abstract: A method for generating a cell support interface for use in a three dimensional cell culture environment, may include electrospinning a mat having an epithelial support layer configured to create an intimate coupling between the epithelial cell and a porous matrix, including a first layer and a second layer, wherein the first layer is formed using a first solution at a first viscosity level and the second layer is formed using a second solution at a second viscosity level different from the first viscosity level.

Abstract: A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.

Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.

Abstract: A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.

Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.

Abstract: The systems and methods of the present disclosure provide techniques for the design and use of an intermediate or transitional plate or block designed to couple acoustic energy at a given frequency from a transducer, such as a piezoelectric transducer, to one or more acoustophoretic devices, such as microfluidic channels, such that driving the chip occurs with a controlled wavelength and symmetry. Such techniques provide improved efficiency when driving a single acoustophoretic device, or for multiple acoustophoretic devices to be operated in concert from a single transducer, and therefore without complex electronics. Additionally, the techniques described herein allow for relaxed design constraints when considering transducer selection and fabrication, instead transferring design constraints to the more easily customized actuation plate.

Abstract: A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.

Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.

Abstract: This invention provides a thermal energy battery having an insulated tank containing a multitude of densely packed plastic tubes filled with a phase-change material (PCM, such as ice) that changes from solid to liquid and vice-versa. Energy is stored when the PCM transitions from liquid to solid form, and released when the PCM transitions back from solid to liquid form. The tubes are arranged vertically, span the height of a well-insulated tank, and are immersed in heat transfer fluid (HTF) contained within the tank. The HTF is an aqueous solution with a freezing point temperature below the freezing point temperature of the chosen PCM. The HTF remains in liquid form at all times during the operation of the battery. Diffusers located allow the HTF to be extracted uniformly from the tank, pumped and cooled by a liquid chiller situated outside the tank and then and inserted back into the tank.

Abstract: This invention provides a thermal energy battery having an insulated tank contains a multitude of densely packed plastic tubes filled with a phase-change material (PCM, such as ice) that changes from solid to liquid and vice-versa. Energy is stored when the PCM transitions from liquid to solid form, and released when the PCM transitions back from solid to liquid form. The tubes are arranged vertically, span the height of a well-insulated tank, and are immersed in heat transfer fluid (HTF) contained within the tank. The HTF is an aqueous solution with a freezing point temperature below the freezing point temperature of the chosen PCM. The HTF remains in liquid form at all times during the operation of the battery. Diffusers located allow the HTF to be extracted uniformly from the tank, pumped and cooled by a liquid chiller situated outside the tank and then and inserted back into the tank.

Abstract: This invention provides a thermal energy battery having an insulated tank contains a multitude of densely packed plastic tubes filled with a phase-change material (PCM, such as ice) that changes from solid to liquid and vice-versa. Energy is stored when the PCM transitions from liquid to solid form, and released when the PCM transitions back from solid to liquid form. The tubes are arranged vertically, span the height of a well-insulated tank, and are immersed in heat transfer fluid (HTF) contained within the tank. The HTF is an aqueous solution with a freezing point temperature below the freezing point temperature of the chosen PCM. The HTF remains in liquid form at all times during the operation of the battery. Diffusers located allow the HTF to be extracted uniformly from the tank, pumped and cooled by a liquid chiller situated outside the tank and then and inserted back into the tank.

Abstract: This invention provides a thermal energy battery having an insulated tank contains a multitude of densely packed plastic tubes filled with a phase-change material (PCM, such as ice) that changes from solid to liquid and vice-versa. Energy is stored when the PCM transitions from liquid to solid form, and released when the PCM transitions back from solid to liquid form. The tubes are arranged vertically, span the height of a well-insulated tank, and are immersed in heat transfer fluid (HTF) contained within the tank. The HTF is an aqueous solution with a freezing point temperature below the freezing point temperature of the chosen PCM. The HTF remains in liquid form at all times during the operation of the battery. Diffusers located allow the HTF to be extracted uniformly from the tank, pumped and cooled by a liquid chiller situated outside the tank and then and inserted back into the tank.

Abstract: A process for synthesizing polymer foam. Polymer foams can be created through the direct mixing of chemicals. Prior research has shown that greater uniformity can be created by physically mixing the components of the foam before and during the foaming process. This invention covers the creation of polymer foam utilizing the mixing energy created by sound waves (herein referred to as sonication) prior to and during the foaming process. The result of physical mixing through sonication is a more uniform foam.