Patents by Inventor Rebecca NUGENT

Rebecca NUGENT has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • COMBINATORIAL DNA ASSEMBLY FOR MULTISPECIFIC ANTIBODIES
    Publication number: 20230340461
    Abstract: Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.
    Type: Application
    Filed: February 13, 2023
    Publication date: October 26, 2023
    Inventors: Aaron SATO, Tom YUAN, Ana G. LUJAN HERNANDEZ, Rebecca NUGENT
  • METHODS AND COMPOSITIONS RELATING TO COVALENTLY CLOSED NUCLEIC ACIDS
    Publication number: 20230115861
    Abstract: Provided herein are methods, systems, and compositions for assembly of covalently closed double stranded nucleic acids. Provided herein are methods, systems, and compositions for assembly covalently closed double stranded nucleic acids for use in various downstream processes.
    Type: Application
    Filed: August 18, 2022
    Publication date: April 13, 2023
    Inventors: Rebecca NUGENT, Siyuan CHEN, Nathan RAYNARD
  • METHODS FOR SEAMLESS NUCLEIC ACID ASSEMBLY
    Publication number: 20220325278
    Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.
    Type: Application
    Filed: April 13, 2022
    Publication date: October 13, 2022
    Inventors: Rebecca NUGENT, Siyuan CHEN, Elian LEE, Nathan RAYNARD
  • BARCODE-BASED NUCLEIC ACID SEQUENCE ASSEMBLY
    Publication number: 20220243195
    Abstract: Provided herein are methods, systems, and compositions for efficient nucleic acid assembly. Nucleic acid assembly may comprise assembly of variants comprising paired homology.
    Type: Application
    Filed: April 15, 2022
    Publication date: August 4, 2022
    Inventors: Rebecca NUGENT, Siyuan CHEN, Ross KETTLEBOROUGH, Elian LEE, Nathan RAYNARD
  • Barcode-based nucleic acid sequence assembly
    Patent number: 11332738
    Abstract: Provided herein are methods, systems, and compositions for efficient nucleic acid assembly. Nucleic acid assembly may comprise assembly of variants comprising paired homology.
    Type: Grant
    Filed: June 19, 2020
    Date of Patent: May 17, 2022
    Assignee: Twist Bioscience Corporation
    Inventors: Rebecca Nugent, Siyuan Chen, Ross Kettleborough, Elian Lee, Nathan Raynard
  • Methods for seamless nucleic acid assembly
    Patent number: 11332740
    Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.
    Type: Grant
    Filed: May 20, 2020
    Date of Patent: May 17, 2022
    Assignee: Twist Bioscience Corporation
    Inventors: Rebecca Nugent, Siyuan Chen, Elian Lee, Nathan Raynard
  • COMPOSITIONS AND METHODS FOR LIBRARY SEQUENCING
    Publication number: 20220106586
    Abstract: Provided herein are compositions and methods for next generation sequencing using universal polynucleotide adapters. Further provided are universal adapters which provide for a large number of samples to be sequenced in parallel. Further provided are methods for high-throughput testing of samples for disease testing.
    Type: Application
    Filed: August 24, 2021
    Publication date: April 7, 2022
    Inventors: Rebecca NUGENT, Siyuan CHEN, Leonardo ARBIZA, Esteban TORO, Michael BOCEK
  • BARCODE-BASED NUCLEIC ACID SEQUENCE ASSEMBLY
    Publication number: 20210071168
    Abstract: Provided herein are methods, systems, and compositions for efficient nucleic acid assembly. Nucleic acid assembly may comprise assembly of variants comprising paired homology.
    Type: Application
    Filed: June 19, 2020
    Publication date: March 11, 2021
    Inventors: Rebecca NUGENT, Siyuan CHEN, Ross KETTLEBOROUGH, Elian LEE, Nathan RAYNARD
  • METHODS FOR SEAMLESS NUCLEIC ACID ASSEMBLY
    Publication number: 20200283760
    Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.
    Type: Application
    Filed: May 20, 2020
    Publication date: September 10, 2020
    Inventors: Rebecca NUGENT, Siyuan CHEN, Elian LEE, Nathan RAYNARD
  • Methods for seamless nucleic acid assembly
    Patent number: 10696965
    Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.
    Type: Grant
    Filed: June 12, 2018
    Date of Patent: June 30, 2020
    Assignee: TWIST BIOSCIENCE CORPORATION
    Inventors: Rebecca Nugent, Siyuan Chen, Elian Lee, Nathan Raynard
  • METHODS FOR SEAMLESS NUCLEIC ACID ASSEMBLY
    Publication number: 20180355351
    Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.
    Type: Application
    Filed: June 12, 2018
    Publication date: December 13, 2018
    Inventors: Rebecca NUGENT, Siyuan CHEN, Elian LEE, Nathan RAYNARD