Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: The present invention provides, in one aspect, an apparatus that comprises a disc-shaped substrate defining (1) a central reservoir region, (2) a plurality of channels in fluid communication with, and emanating substantially radially from, the central reservoir region, the channels being coplanar with each other, and each channel having (i) a proximal end which is linked to the reservoir region, and (ii) a distal end, and preferably (3) for each channel, at least one chamber, and preferably three chambers, linked by a passageway in fluid communication with the distal end of that channel.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device can comprise wells and each well can comprise an analyte-specific reagent effective to react with a selected analyte that may be present in the sample. Also disclosed are methods utilizing the device.

Abstract: The present teachings provide a microfluidic apparatus comprising a body defining at least one channel that extends through said body, the channel including an inlet and an outlet; and an electrode positioned in proximity to the body and configured to provide an electrical field near the outlet of the channel to at least partially confine an eluted sample component passing from the channel. The present teachings also provide an electrophoresis system, comprising a microfluidic chip defining a plurality of channels passing therethrough, each of the channels having an inlet and an outlet; a first electrode in electrical communication with the inlet of each of the channels; a second electrode in electrical communication with the outlet of each of the channels; and a third electrode in electrical communication with the outlet of each of the channels, wherein the third electrode is positioned to provide an electrical field to at least partially confine an eluted sample component passing from any of the channels.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: A method for making a test device is provided. The method can comprise providing (a) a substrate comprising a plurality of wells, wherein each well comprises at least one dimension no greater than 1 mm; (ii) a robotic dispensing system comprising at least one piezoelectric dispenser; and (iii) a liquid solution comprising at least one analyte-specific reagent. The method further comprises delivering a volume of the liquid solution into at least some of the wells using the at least one piezoelectric dispenser. The volume can be no greater than about 1 microliter. The method can further comprise placing a cover over the wells.

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract: Generally, the present invention provides a device and method for accurately locating a multi-well plate at a plate-support location, e.g., a work surface, of an automated laboratory machine, such that one or more acting members of the machine, such as an array of pipette tips, optical sensors, or the like, can accurately address and operate on the individual wells. Interior and/or exterior surface regions of one or more wells of a multi-well plate are used as the primary plate features engaged by locating structure of the machine. In one particular embodiment one or more upwardly tapered projections extend from a plate-support surface of a plate-handling machine for mating engagement with the exterior surface regions of one or more wells. In another embodiment, the exterior surface regions along one or more well bottoms are engagingly received within bores, or other receiving structure, formed on the plate-support surface of a machine.

Abstract: Bubble-free electrodes, electrochemical cells including bubble-free electrodes, analytical devices, and methods for preparing and using them are provided. The analytical devices each include at least one bubble-free electrode. Analytical devices that include an electrochemical cell and a sample containment device are also provided, wherein the electrochemical cell includes an anodic reservoir, a cathodic reservoir, an electrical connection between the anodic reservoir and the cathodic reservoir, and a first bubble-free electrode disposed within one of the anodic reservoir and the cathodic reservoir. A second electrode is disposed within the other reservoir and a power source is provided having a positive terminal that is normally in electrical contact with the first electrode, and a negative terminal that is normally in electrical contact with the second electrode.

Abstract: The present teachings provide a microfluidic apparatus comprising a body defining at least one channel that extends through said body, the channel including an inlet and an outlet; and an electrode positioned in proximity to the body and configured to provide an electrical field near the outlet of the channel to at least partially confine an eluted sample component passing from the channel. The present teachings also provide an electrophoresis system, comprising a microfluidic chip defining a plurality of channels passing therethrough, each of the channels having an inlet and an outlet; a first electrode in electrical communication with the inlet of each of the channels; a second electrode in electrical communication with the outlet of each of the channels; and a third electrode in electrical communication with the outlet of each of the channels, wherein the third electrode is positioned to provide an electrical field to at least partially confine an eluted sample component passing from any of the channels.