Patents by Inventor Rekha Seshadri
Rekha Seshadri has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220186236Abstract: The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.Type: ApplicationFiled: February 28, 2022Publication date: June 16, 2022Inventors: Rekha Seshadri, Ariel S. Schwartz, Leah Soriaga, Robert C. Brown
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Patent number: 11261452Abstract: The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.Type: GrantFiled: January 23, 2018Date of Patent: March 1, 2022Assignee: Viridos, Inc.Inventors: Rekha Seshadri, Ariel S. Schwartz, Leah Soriaga, Robert C. Brown
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Patent number: 10612034Abstract: The present invention provides novel promoter and terminator sequences for use in heterologous gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a heterologous gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the heterologous gene; and methods for identifying promoters.Type: GrantFiled: June 1, 2012Date of Patent: April 7, 2020Assignee: EXXONMOBIL RESEARCH AND ENGINEERING COMPANYInventors: Jane C. Schneider, Bo Liu, Rekha Seshadri
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Publication number: 20180155731Abstract: The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.Type: ApplicationFiled: January 23, 2018Publication date: June 7, 2018Inventors: Rekha Seshadri, Ariel S. Schwartz, Leah Soriaga, Robert C. Brown
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Patent number: 9914931Abstract: The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.Type: GrantFiled: December 5, 2013Date of Patent: March 13, 2018Assignee: Synthetic Genomics, Inc.Inventors: Rekha Seshadri, Ariel S. Schwartz, Leah Soriaga, Robert C. Brown
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Patent number: 9181568Abstract: The invention relates to methods for producing lipids such as fatty acid products in recombinant host cells engineered to express a non-native gene encoding a dehydrogenase. The recombinant microorganisms are able to proliferate at a higher rate as compared with microorganisms that do not express a non-native dehydrogenase gene, and cultures of microorganisms that are engineered for lipid production and that express a non-native dehydrogenase produce more lipid than cultures of control microorganisms that do not include a non-native dehydrogenase gene.Type: GrantFiled: April 23, 2012Date of Patent: November 10, 2015Assignee: ExxonMobil Research and Engineering CompanyInventors: Robert Christopher Brown, Jennifer Coppersmith, Prachee Prakash, Srividya Akella, Rekha Seshadri
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Patent number: 9096834Abstract: The present invention provides for the improved production of fatty acid products in a recombinant microorganism or host cell engineered to express a non-native gene encoding a thioesterase and a non-native gene encoding a lysophosphatidic acid acyltransferase (LPAAT), in which the LPAAT is overexpressed in the recombinant microorganism compared to an otherwise identical microorganism that does not include the non-native LPAAT gene. The invention also provides methods of producing fatty acid products using such recombinant microorganisms.Type: GrantFiled: February 24, 2012Date of Patent: August 4, 2015Assignee: EXXONMOBIL RESEARCH AND ENGINEERING COMPANYInventors: Rekha Seshadri, Jennie Lau Kit, Nicholas Bauman, Robert Christopher Brown
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Patent number: 9040264Abstract: The invention provides microorganisms such as cyanobacteria genetically engineered to express proteins that include transcription factor domains for upregulation of lipid biosynthetic pathways. In addition to expression a gene encoding a transcription factor domain protein, the recombinant microorganisms can express at least one exogenous gene that encodes a polypeptide for the production of a fatty acid, fatty acid derivative, or triglyceride. Also included are methods of producing a fatty acid, fatty acid derivative, or triglyceride using the engineered microorganisms described herein as well as nucleic acid molecules encoding novel transcription factor domain proteins.Type: GrantFiled: March 30, 2012Date of Patent: May 26, 2015Assignee: EXXONMOBIL RESEARCH AND ENGINEERING COMPANYInventors: Jessica Kristof, Rekha Seshadri
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Patent number: 9017975Abstract: The invention relates to acyl-CoA-independent methods of producing fatty alcohols in recombinant host cells engineered to express an alcohol-forming acyl-ACP reductase. The recombinant host cells may be photosynthetic microorganisms, such as cyanobacteria. Isolated nucleic acid molecules, vectors, and recombinant host cells expressing an alcohol-forming acyl-ACP reductase, and systems for producing fatty alcohols via an acyl-CoA-independent pathway, are also provided. Also provided are microorganisms engineered for the secretion of fatty acids and fatty acid derivatives, including fatty alcohols, and methods of producing fatty acid derivatives using such engineering microorganisms.Type: GrantFiled: September 27, 2012Date of Patent: April 28, 2015Assignee: Synthetic Genomics, Inc.Inventors: Erik Holtzapple, Robert C. Brown, Rekha Seshadri, Jennifer Coppersmith, John Verruto
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Patent number: 8846370Abstract: The described invention provides genetically engineered microorganisms, including photosynthetic microorganisms, expressing 4-hydroxybenzoyl-CoA thioesterases and methods of using the genetically engineered microorganisms for producing free fatty acids and/or fatty acid derivatives.Type: GrantFiled: December 13, 2011Date of Patent: September 30, 2014Assignee: ExxonMobil Research and Engineering CompanyInventors: Robert Christopher Brown, Rekha Seshadri, Carlos Chavez-Torres, Weidong Xu, Toby Richardson
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Patent number: 8835149Abstract: The present invention provides novel diacylglycerol acyltransferase (DGAT) genes comprising Pleckstrin Homology (PH) domains. The present invention also provides for recombinant cells, such as algae, transformed with acyltransferase genes, such as DGAT, comprising PH domains, and methods of using such recombinant cells to produce increased triglyceride levels.Type: GrantFiled: December 6, 2012Date of Patent: September 16, 2014Assignee: ExxonMobil Research and Engineering CompanyInventors: Jennifer Coppersmith, Rekha Seshadri, Toby Howard Richardson
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Publication number: 20140186842Abstract: The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.Type: ApplicationFiled: December 5, 2013Publication date: July 3, 2014Applicant: Synthetic Genomics, Inc.Inventors: Rekha Seshadri, Ariel S. Schwartz, Leah Soriaga, Robert C. Brown
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Publication number: 20140162329Abstract: The present invention provides novel diacylglycerol acyltransferase (DGAT) genes comprising Pleckstrin Homology (PH) domains. The present invention also provides for recombinant cells, such as algae, transformed with acyltransferase genes, such as DGAT, comprising PH domains, and methods of using such recombinant cells to produce increased triglyceride levels.Type: ApplicationFiled: December 6, 2012Publication date: June 12, 2014Applicant: ExxonMobil Research and Engineering CompanyInventors: Jennifer Coppersmith, Rekha Seshadri, Toby Howard Richardson
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Publication number: 20130323780Abstract: The present invention provides novel promoter and terminator sequences for use in heterologous gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a heterologous gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the heterologous gene; and methods for identifying promoters.Type: ApplicationFiled: June 1, 2012Publication date: December 5, 2013Applicant: EXXONMOBIL RESEARCH AND ENGINEERING COMPANYInventors: JANE C. SCHNEIDER, BO LIU, REKHA SESHADRI
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Publication number: 20130280793Abstract: The invention relates to methods for producing lipids such as fatty acid products in recombinant host cells engineered to express a non-native gene encoding a dehydrogenase. The recombinant microorganisms are able to proliferate at a higher rate as compared with microorganisms that do not express a non-native dehydrogenase gene, and cultures of microorganisms that are engineered for lipid production and that express a non-native dehydrogenase produce more lipid than cultures of control microorganisms that do not include a non-native dehydrogenase gene.Type: ApplicationFiled: April 23, 2012Publication date: October 24, 2013Applicant: ExxonMobil Research and Engineering CompanyInventors: Robert Christopher Brown, Jennifer Coppersmith, Prachee Prakash, Srividya Akella, Rekha Seshadri
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Patent number: 8530207Abstract: The described invention provides genetically engineered photosynthetic microorganisms expressing prokaryotic acyl-ACP thioesterases and methods of using the genetically engineered photosynthetic microorganisms for producing free fatty acids and/or fatty acid derivatives.Type: GrantFiled: December 13, 2011Date of Patent: September 10, 2013Assignee: ExxonMobil Research and Engineering CompanyInventors: Kevin Watts, Rekha Seshadri, Toby Richardson
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Publication number: 20130224810Abstract: The present invention provides for the improved production of fatty acid products in a recombinant microorganism or host cell engineered to express a non-native gene encoding a thioesterase and a non-native gene encoding a lysophosphatidic acid acyltransferase (LPAAT), in which the LPAAT is overexpressed in the recombinant microorganism compared to an otherwise identical microorganism that does not include the non-native LPAAT gene. The invention also provides methods of producing fatty acid products using such recombinant microorganisms.Type: ApplicationFiled: February 24, 2012Publication date: August 29, 2013Applicant: ExxonMobil Research and Engineering CompanyInventors: Rekha Seshadri, Jennie Lau Kit, Nicholas Bauman, Robert Christopher Brown
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Publication number: 20120252080Abstract: The invention provides microorganisms such as cyanobacteria genetically engineered to express proteins that include transcription factor domains for upregulation of lipid biosynthetic pathways. In addition to expression a gene encoding a transcription factor domain protein, the recombinant microorganisms can express at least one exogenous gene that encodes a polypeptide for the production of a fatty acid, fatty acid derivative, or triglyceride. Also included are methods of producing a fatty acid, fatty acid derivative, or triglyceride using the engineered microorganisms described herein as well as nucleic acid molecules encoding novel transcription factor domain proteins.Type: ApplicationFiled: March 30, 2012Publication date: October 4, 2012Applicant: EXXONMOBIL RESEARCH AND ENGINEERING COMPANYInventors: Jessica Kristof, Rekha Seshadri
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Publication number: 20120164713Abstract: The described invention provides genetically engineered microorganisms, including photosynthetic microorganisms, expressing 4-hydroxybenzoyl-CoA thioesterases and methods of using the genetically engineered microorganisms for producing free fatty acids and/or fatty acid derivatives.Type: ApplicationFiled: December 13, 2011Publication date: June 28, 2012Applicant: ExxonMobil Research and Engineering CompanyInventors: Robert Christopher Brown, Rekha Seshadri, Carlos Chavez-Torres, Weidong Xu, Toby Richardson
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Publication number: 20120164700Abstract: The described invention provides genetically engineered photosynthetic microorganisms expressing prokaryotic acyl-ACP thioesterases and methods of using the genetically engineered photosynthetic microorganisms for producing free fatty acids and/or fatty acid derivatives.Type: ApplicationFiled: December 13, 2011Publication date: June 28, 2012Applicant: ExxonMobil Research and Engineering CompanyInventors: Kevin Watts, Rekha Seshadri, Toby Richardson