Patents by Inventor Remigijus Skirgaila
Remigijus Skirgaila has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11560553Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: GrantFiled: April 8, 2020Date of Patent: January 24, 2023Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
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Publication number: 20200325460Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: ApplicationFiled: April 8, 2020Publication date: October 15, 2020Inventors: Remigijus SKIRGAILA, Renata RIMSELIENE, Rasa SUKACKAITE, Martyna SIMUTYTE, Skaiste VALINSKYTE, Karolis MATJOSAITIS, Mindaugas UKANIS
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Patent number: 10626383Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: GrantFiled: January 13, 2017Date of Patent: April 21, 2020Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
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Patent number: 10590399Abstract: A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.Type: GrantFiled: September 29, 2016Date of Patent: March 17, 2020Assignee: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Agne Tubelevic̆iūtē, Renata Rims̆eliēnē, Sigitas Burinskas
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Patent number: 10358670Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: April 20, 2018Date of Patent: July 23, 2019Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Patent number: 10287614Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: May 2, 2017Date of Patent: May 14, 2019Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20180298414Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: April 20, 2018Publication date: October 18, 2018Applicant: Thermo Fisher Scientific Baltics UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Patent number: 9885025Abstract: Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template.Type: GrantFiled: July 14, 2016Date of Patent: February 6, 2018Assignee: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Tadas Povilaitis
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Publication number: 20170298403Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain n o more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: May 2, 2017Publication date: October 19, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20170204384Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: ApplicationFiled: January 13, 2017Publication date: July 20, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
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Patent number: 9683251Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: June 19, 2013Date of Patent: June 20, 2017Assignee: Thermo Fisher Scientific Baltics UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20170081646Abstract: A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.Type: ApplicationFiled: September 29, 2016Publication date: March 23, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Agne Tubeleviciute, Renata Rimseliene, Sigitas Burinskas
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Publication number: 20170015980Abstract: Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template.Type: ApplicationFiled: July 14, 2016Publication date: January 19, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Tadas Povilaitis
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Patent number: 9493848Abstract: The invention provides novel mutant thermostable DNA polymerases having desirable properties such as increased polymerase efficiency and speed, increased affinity to DNA substrates and/or increased resistance to DNA polymerase inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel DNA polymerases.Type: GrantFiled: August 2, 2012Date of Patent: November 15, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Agne Tubelevi{hacek over (c)}iūté, Renata Rim{hacek over (s)}eliėnė, Sigitas Burinskas
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Patent number: 9422535Abstract: Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template.Type: GrantFiled: December 20, 2013Date of Patent: August 23, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Tadas Povilaitis
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Patent number: 9243233Abstract: A restriction endonuclease with a recognition sequence 5?-TCGA-3?. The restriction endonuclease is sensitive to the presence of a modified cytosine residue in the recognition sequence. Methods and kits using the restriction endonuclease with a recongition sequence 5?-TCGA-3? are also disclosed.Type: GrantFiled: July 1, 2013Date of Patent: January 26, 2016Assignee: Thermo Fisher Scientific UABInventors: Renata Rim{hacek over (s)}elienė, Remigijus Skirgaila, Arvydas Lubys
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Publication number: 20140322759Abstract: Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template.Type: ApplicationFiled: December 20, 2013Publication date: October 30, 2014Inventors: Remigijus Skirgaila, Tadas Povilaitis
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Patent number: 8835148Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: October 7, 2010Date of Patent: September 16, 2014Assignee: Thermo Fisher Scientific Baltics, UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20140011196Abstract: A restriction endonuclease with a recognition sequence 5?-TCGA-3?. The restriction endonuclease is sensitive to the presence of a modified cytosine residue in the recognition sequence. Methods and kits using the restriction endonuclease with a recongition sequence 5?-TCGA-3? are also disclosed.Type: ApplicationFiled: July 1, 2013Publication date: January 9, 2014Inventors: Renata Rimseliene, Remigijus Skirgaila, Arvydas Lubys
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Patent number: 8580548Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: February 29, 2012Date of Patent: November 12, 2013Assignee: Thermo Fisher Scientific Baltics, UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene