Abstract: A machine for opening simultaneously a plurality of blood segments comprising a number of segment carriers laterally co-joined and having therein strictly located apertures, an attached needle guide having perforations aligned with the carrier apertures and a spring-returned handle assembly containing captive needles to the same number as, and aligned with, the carrier apertures and guide perforations. Activation of the handle forces the needles to penetrate all blood segments in the carriers. A base tray is provided to contain any possible leakage from the segments.