Patents by Inventor René Marcel De Jong
René Marcel De Jong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10253305Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.Type: GrantFiled: October 12, 2017Date of Patent: April 9, 2019Assignee: DSM IP ASSETS B.V.Inventors: Petrus Jacobus Theodorus Dekker, Rene Marcel De Jong, Cornelis Marinus Muijlwijk
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Patent number: 10167463Abstract: The invention relates to a polypeptide having chymosin activity which: a. is capable of hydrolyzing bovine alpha s1-casein at position F23F24 so as to form ?s1-I CN (f24-199) more rapidly than camel chymosin; and b. has a C/P ratio higher than the C/P ratio of bovine chymosin.Type: GrantFiled: May 3, 2013Date of Patent: January 1, 2019Assignee: DSM IP ASSETS B.V.Inventors: Petrus Jacobus Theodorus Dekker, Rene Marcel De Jong, Michael Dennis Tabeling, Cornelis Marinus Muijlwijk
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Publication number: 20180320156Abstract: The invention relates to a variant polypeptide having lactase activity. The invention also relates to a nucleic acid sequence encoding such a variant polypeptide, to a nucleic acid construct comprising said nucleic acid sequence, to a recombinant expression vector comprising said nucleic acid construct and to a recombinant host cell comprising said expression vector. Further, the invention relates to a method for producing a lactase variant via use of such a host cell. Also, the invention relates to a method of producing a lactase polypeptide variant. The invention further relates to a composition comprising a lactase variant, to use of such a lactase variant or to the use of a lactase variant-containing composition in the preparation of a dairy product, to a process for the production of a dairy product and to the resulting dairy product.Type: ApplicationFiled: May 4, 2018Publication date: November 8, 2018Inventors: René Marcel DE JONG, Ulrike Maria MÜLLER, Petrus Jacobus Theodorus DEKKER
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Patent number: 10100320Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.Type: GrantFiled: June 2, 2017Date of Patent: October 16, 2018Assignee: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Rene Marcel De Jong
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Publication number: 20180245059Abstract: The invention relates to a polypeptide having hemicellulase activity. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: April 25, 2018Publication date: August 30, 2018Inventors: Alrik Pieter LOS, Rene Marcel DE JONG, Maaike APPELDOORN
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Patent number: 10000537Abstract: The invention relates to a polypeptide having one or more substitution at a position corresponding to position 339 or 376 of SEQ ID NO: 59, wherein the polypeptide is a member of the Major Facilitator Superfamily (MFS). In an embodiment, the substitution is at position corresponding to 376 and wherein the amino acid at that position is replaced by an amino acid that has a van der Waals volume of 80 to 138 ?3 and a side chain hydrophobicity of 10 to 100 ?tR.Type: GrantFiled: June 4, 2014Date of Patent: June 19, 2018Assignee: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Paulus Petrus De Waal, Rene Marcel De Jong, Arnold Jacob Mattieu Driessen, Jeroen Gerben Nijland, Hyun Yong Shin
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Patent number: 9994837Abstract: The invention relates to a variant polypeptide having lactase activity. The invention also relates to a nucleic acid sequence encoding such a variant polypeptide, to a nucleic acid construct comprising said nucleic acid sequence, to a recombinant expression vector comprising said nucleic acid construct and to a recombinant host cell comprising said expression vector. Further, the invention relates to a method for producing a lactase variant via use of such a host cell. Also, the invention relates to a method of producing a lactase polypeptide variant. The invention further relates to a composition comprising a lactase variant, to use of such a lactase variant or to the use of a lactase variant-containing composition in the preparation of a dairy product, to a process for the production of a dairy product and to the resulting dairy product.Type: GrantFiled: January 13, 2015Date of Patent: June 12, 2018Assignee: DSM IP ASSETS B.V.Inventors: René Marcel De Jong, Ulrike Maria Müller, Petrus Jacobus Theodorus Dekker
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Patent number: 9988615Abstract: The invention relates to a polypeptide having hemicellulase activity. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: GrantFiled: February 3, 2014Date of Patent: June 5, 2018Assignee: DSM IP ASSETS B.V.Inventors: Alrik Pieter Los, Rene Marcel De Jong, Maaike Appeldoorn
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Publication number: 20180134986Abstract: The present invention relates to a polypeptide having phospholipase C activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1.Type: ApplicationFiled: April 8, 2016Publication date: May 17, 2018Applicant: DSM IP ASSETS B.V.Inventors: Arjen SEIN, Rene Marcel DE JONG, Evert Tjeerd VAN RIJ, Willem BIJLEVELD, Maarten Hotse WILBRINK
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Patent number: 9944905Abstract: The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.Type: GrantFiled: December 12, 2014Date of Patent: April 17, 2018Assignee: DSM IP ASSETS B.V.Inventors: René Marcel De Jong, Zheng Zhao, Ben Den Dulk, Remko Tsjibbe Winter
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Publication number: 20180030427Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.Type: ApplicationFiled: October 12, 2017Publication date: February 1, 2018Inventors: Petrus Jacobus Theodorus DEKKER, Rene Marcel De Jong, Cornelis Marinus Muijlwijk
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Publication number: 20180007917Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue. Such a variant polypeptide may be used in the preparation of a baked product.Type: ApplicationFiled: September 26, 2017Publication date: January 11, 2018Inventors: Rene Marcel DE JONG, Hanna Elsabet ABBAS, Evert Tjeerd VAN RIJ
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Patent number: 9822352Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.Type: GrantFiled: May 3, 2013Date of Patent: November 21, 2017Assignee: DSM IP ASSETS B.V.Inventors: Petrus Jacobus Theodorus Dekker, Rene Marcel De Jong, Cornelis Marinus Muijlwijk
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Patent number: 9808018Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue. Such a variant polypeptide may be used in the preparation of a baked product.Type: GrantFiled: February 27, 2014Date of Patent: November 7, 2017Assignee: DSM IP ASSETS B.V.Inventors: Rene Marcel De Jong, Hanna Elisabet Abbas, Evert Tjeerd Van Rij
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Publication number: 20170268012Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.Type: ApplicationFiled: June 2, 2017Publication date: September 21, 2017Inventors: Paul KLAASSEN, Gijsberdina Pieternella Van Suylekom, Rene Marcel De Jong
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Patent number: 9701972Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.Type: GrantFiled: August 2, 2012Date of Patent: July 11, 2017Assignee: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Rene Marcel De Jong
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Publication number: 20170029476Abstract: The present invention relates to a nucleic acid construct comprising: a nucleic acid sequence encoding an ice structuring protein (ISP) comprising the sequence set out in SEQ ID NO: 1 or a sequence at least 80% identical thereto or comprising the sequence set out in amino acids 21 to 261 of SEQ ID NO: 1 or a sequence at least 80% identical thereto; and, linked operably thereto, control sequences permitting expression of the nucleic acid sequence in a filamentous fungal host cell. The construct may be used in a method for the production of an ISP, wherein the ISP is expressed in a filamentous fungal host cell.Type: ApplicationFiled: December 2, 2014Publication date: February 2, 2017Applicant: DSM IP ASSETS B.V.Inventors: René Marcel DE JONG, Petrus Jacobus THEODORUS
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Publication number: 20160333331Abstract: The invention relates to a variant polypeptide having lactase activity. The invention also relates to a nucleic acid sequence encoding such a variant polypeptide, to a nucleic acid construct comprising said nucleic acid sequence, to a recombinant expression vector comprising said nucleic acid construct and to a recombinant host cell comprising said expression vector. Further, the invention relates to a method for producing a lactase variant via use of such a host cell. Also, the invention relates to a method of producing a lactase polypeptide variant. The invention further relates to a composition comprising a lactase variant, to use of such a lactase variant or to the use of a lactase variant-containing composition in the preparation of a dairy product, to a process for the production of a dairy product and to the resulting dairy product.Type: ApplicationFiled: January 13, 2015Publication date: November 17, 2016Applicant: DSM IP ASSETS B.V.Inventors: René Marcel DE JONG, Ulrike Maria MÜLLER, Petrus Jacobus Theodorus DEKKER
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Publication number: 20160319245Abstract: The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.Type: ApplicationFiled: December 12, 2014Publication date: November 3, 2016Inventors: René Marcel DE JONG, Zheng ZHAO, Ben DEN DULK, Remko Tsjibbe WINTER
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Patent number: 9441214Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: GrantFiled: June 23, 2011Date of Patent: September 13, 2016Assignee: DSM IP ASSETS B.V.Inventors: Margot Elisabeth Francoise Schoonneveld-Bergmans, Wilbert Herman Marie Heijne, Rene Marcel De Jong, Robbertus Antonius Damveld