Abstract: The present disclosure discloses an enhancing agent and a kit for enhancing nucleic acid amplification reaction and a method for performing nucleic acid amplification reaction, relating to the field of biotechnology. The enhancing agent disclosed in the present disclosure has a domain capable of binding to fluorescent dye and a quenching group for quenching fluorescence emitted by the fluorescent dye bound to the domain binds. As the binding affinity between the above domain and the fluorescent dye is lower than the binding affinity between a target amplification product of nucleic acid amplification reaction and the fluorescent dye, the enhancing agent not only can reduce inhibition of high-concentration fluorescent dye to amplification reaction, but also can enhance signal intensity of the fluorescent dye in the nucleic acid amplification reaction based on microfluidic chip, improving reliability of application of the fluorescent dye as amplification indicator on the microfluidic chip.

Abstract: Provided is an apparatus, system and method for on-chip microfluids dispensing. The apparatus comprising a substrate; a plurality of first electrodes arranged one next to another on the substrate; a dielectric layer above and enclosing the plurality of first electrodes; and a second electrode on the substrate, wherein each of the plurality of first electrodes is in electric communication with a respective first driving signal input; wherein the second electrode is in electric communication with a second driving signal input; wherein the plurality of first electrodes define a continuous fluid path along a longitudinal direction for retaining microfluids, and wherein the second electrode is arranged within the continuous fluid path and defines a jetting position and an adjacent mixing position within the continuous fluid path.

Abstract: The present disclosure discloses an enhancing agent and a kit for enhancing nucleic acid amplification reaction and a method for performing nucleic acid amplification reaction, relating to the field of biotechnology. The enhancing agent disclosed in the present disclosure has a domain capable of binding to fluorescent dye and a quenching group for quenching fluorescence emitted by the fluorescent dye bound to the domain binds. As the binding affinity between the above domain and the fluorescent dye is lower than the binding affinity between a target amplification product of nucleic acid amplification reaction and the fluorescent dye, the enhancing agent not only can reduce inhibition of high-concentration fluorescent dye to amplification reaction, but also can enhance signal intensity of the fluorescent dye in the nucleic acid amplification reaction based on microfluidic chip, improving reliability of application of the fluorescent dye as amplification indicator on the microfluidic chip.

Abstract: Provided is an apparatus, system and method for on-chip microfluids dispensing. The apparatus comprising a substrate; a plurality of first electrodes arranged one next to another on the substrate; a dielectric layer above and enclosing the plurality of first electrodes; and a second electrode on the substrate, wherein each of the plurality of first electrodes is in electric communication with a respective first driving signal input; wherein the second electrode is in electric communication with a second driving signal input; wherein the plurality of first electrodes define a continuous fluid path along a longitudinal direction for retaining microfluids, and wherein the second electrode is arranged within the continuous fluid path and defines a jetting position and an adjacent mixing position within the continuous fluid path.