Abstract: A biomolecule analysis method of the present disclosure includes: preparing a biomolecule analysis device including a thin film, a first liquid tank and a second liquid tank separated by the thin film, a first electrode disposed in the first liquid tank, and a second electrode disposed in the second liquid tank; and forming a nanopore in the thin film by applying a first voltage between the first electrode and the second electrode in a state where a nanopore forming solution is enclosed in the first liquid tank and the second liquid tank, wherein the nanopore forming solution contains ammonium ions and sulfate ions.

Abstract: Provided is a method for generating a trained model for classifying blocking event data representing nanopore blocking events in a biomolecule measurement device. The method includes generating a first trained model by executing machine learning of a training model using first teacher data, the first teacher data includes teacher blocking event data and a teacher label, the teacher label indicates whether the teacher blocking event data is classified as Good data or bad data, and the first trained model is configured to classify the blocking event data into good data or bad data. In addition, a method for determining a base sequence a biomolecule and a biomolecule measurement device are provided.

Abstract: A biomolecule is more easily and reliably reciprocated in a nanopore. An adapter molecule that directly or indirectly binds to a biomolecule to be analyzed comprises a three-dimensional structure formation domain consisting of a single-stranded nucleotide.

Abstract: A double-stranded DNA to be analyzed is analyzed without subjecting it to a modification treatment. An adapter molecule to be bound to the double-stranded DNA to be analyzed has a double-stranded nucleic acid region having base sequences complementary to each other, a pair of single-stranded nucleic acid regions having base sequences non-complementary to each other, and a block molecule placed in one of the single-stranded nucleic acid regions.

Abstract: Provided are a first tank; a second tank; a thin film having a nanopore, which communicates the first tank to the second tank, and disposed between the first and second tanks; a first electrode provided in the first tank; and a second electrode provided in the second tank. A wall surface of the nanopore has an ion adsorption preventing structure to prevent desorption/adsorption of an ion contained in a solution filling the first tank and/or the second tank, and a voltage is applied between the first and second electrodes to measure an ion current flowing through the nanopore.

Abstract: A pore forming method in which a pore is formed in such a way that a first voltage is applied between electrodes that are disposed with a film in an electrolytic solution therebetween; a second voltage, which is lower than the first voltage, is applied between the electrodes; a current that flows between the electrodes owing to the application of the second voltage is measured; it is judged whether a value of a current is equal to or larger than a predefined threshold; and if the value of the current is smaller than the threshold, the above sequence is repeated until a pore is formed. In this case, the second voltage is a voltage that makes the value (IPF) of the current flowing through the film practically 0. With the use of the above method, a nanopore is formed in the film simply, easily, and accurately.

Abstract: While an insulating film having a near-field light generating element placed thereon is being irradiated with light in an electrolytic solution, or after the film that has been irradiated with light is disposed in the electrolytic solution, a first voltage is applied between the two electrodes installed in the electrolytic solution across the film, a second voltage is then applied between the two electrodes, and a value of a current that flows between the two electrodes due to the application of the second voltage is detected. This procedure is stopped when the current value reaches or exceeds a pre-set threshold value, whereby a hole is formed at a desired location in the thin-film.

Abstract: To introduce a biomolecule into a nanopore without the need to check the position of the nanopore in a thin film. In addition, displacement stability is ensured and stable acquisition of blocking signals is realized. An immobilization member 107 having a larger size than a thin film 113 with a nanopore 112 is used, and biomolecules are immobilized on the biomolecule immobilization member 107 at a density that allows at least one biomolecule 108 to enter an electric field region around the nanopore when the biomolecule immobilization member 107 has moved close to a nanopore device 101.

Abstract: A biomolecule analysis device includes a thin film having a nanopore, a liquid tank that is disposed in contact with the thin film and contains an electrolyte solution, an electrode in contact with the liquid tank, a measurement device connected to the electrode, and a controller that controls a voltage to be applied to the electrode, in accordance with a measurement result of the measurement device. A biomolecule is introduced into the electrolyte solution. A control strand and a molecular motor are connected to a first end portion of the biomolecule, and the control strand is bound to a primer on an upstream of the control strand and has a spacer on a downstream of the control strand.

Abstract: A pore forming method in which a pore is formed in such a way that a first voltage is applied between electrodes that are disposed with a film in an electrolytic solution therebetween; a second voltage, which is lower than the first voltage, is applied between the electrodes; a current that flows between the electrodes owing to the application of the second voltage is measured; it is judged whether a value of a current is equal to or larger than a predefined threshold; and if the value of the current is smaller than the threshold, the above sequence is repeated until a pore is formed. In this case, the second voltage is a voltage that makes the value (IPF) of the current flowing through the film practically 0. With the use of the above method, a nanopore is formed in the film simply, easily, and accurately.

Abstract: A biomolecule measuring device includes a first liquid tank and a second liquid tank which are filled with an electrolytic solution, a nanopore device that supports a thin film having a nanopore and is provided between the first liquid tank and the second liquid tank so as to communicate between the first liquid, tank and the second liquid tank through the nanopores, and an immobilizing member that is disposed in the first liquid tank, has a size larger than that of the thin film, and to which the biomolecules are immobilized, in which at least, one of the nanopore device and the immobilizing member has a groove structure.

Abstract: A pore forming method in which a pore is formed in such a way that a first voltage is applied between electrodes that are disposed with a film in an electrolytic solution therebetween; a second voltage, which is lower than the first voltage, is applied between the electrodes; a current that flows between the electrodes owing to the application of the second voltage is measured; it is judged whether a value of a current is equal to or larger than a predefined threshold; and if the value of the current is smaller than the threshold, the above sequence is repeated until a pore is formed. In this case, the second voltage is a voltage that makes the value (IPF) of the current flowing through the film practically 0. With the use of the above method, a nanopore is formed in the film simply, easily, and accurately.

Abstract: Provided are a first tank; a second tank; a thin film having a nanopore, which communicates the first tank to the second tank, and disposed between the first and second tanks; a first electrode provided in the first tank; and a second electrode provided in the second tank. A wall surface of the nanopore has an ion adsorption preventing structure to prevent desorption/adsorption of an ion contained in a solution filling the first tank and/or the second tank, and a voltage is applied between the first and second electrodes to measure an ion current flowing through the nanopore.

Abstract: To introduce a biomolecule into a nanopore without the need to check the position of the nanopore in a thin film. In addition, displacement stability is ensured and stable acquisition of blocking signals is realized. An immobilization member 107 having a larger size than a thin film 113 with a nanopore 112 is used, and biomolecules are immobilized on the biomolecule immobilization member 107 at a density that allows at least one biomolecule 108 to enter an electric field region around the nanopore when the biomolecule immobilization member 107 has moved close to a nanopore device 101.

Abstract: To introduce a biomolecule into a nanopore without the need to check the position of the nanopore in a thin film. In addition, displacement stability is ensured and stable acquisition of blocking signals is realized. An immobilization member 107 having a larger size than a thin film 113 with a nanopore 112 is used, and biomolecules are immobilized on the biomolecule immobilization member 107 at a density that allows at least one biomolecule 108 to enter an electric field region around the nanopore when the biomolecule immobilization member 107 has moved close to a nanopore device 101.

Abstract: The purpose of the present invention is to provide a DNA transport control device having excellent reliability and durability, and a DNA sequencing device that uses the DNA transport control device. The present invention provides a DNA transport control device having a nanopore which allows for the passage of only the DNA strand of a single molecule, and a DNA sequencing device that uses the DNA transport control device. The DNA transport control device is characterized by the following: including a base material having openings and a thin film a block copolymer formed on the base material; the thin film including microdomains that are formed as a result of self-assembly of the block copolymer and that penetrate the thin film, and a matrix surrounding the microdomains; and the nanopore being formed from one opening in the base material and a single microdomain.

Abstract: A traditional nanopore DNA sequencing method has a problem in that a signal analysis error may occur when a signal variation reflecting fluctuation in a base current is contained in a signal variation in a signal analysis. An electrolyte solution for biomolecule assays, containing D2O as a solvent, and/or containing an electrolyte that has Cs and Na, Na alone, Na and Li, or Li alone as a cation species in the electrolyte solution, or trishydroxyaminomethane, or a combination thereof is used in formation of a nanopore or in measurement.

Abstract: A biomolecule measuring device includes a first liquid tank and a second liquid tank which are filled with an electrolytic solution, a nanopore device that supports a thin film having a nanopore and is provided between the first liquid tank and the second liquid tank so as to communicate between the first liquid, tank and the second liquid tank through the nanopores, and an immobilizing member that is disposed in the first liquid tank, has a size larger than that of the thin film, and to which the biomolecules are immobilized, in which at least, one of the nanopore device and the immobilizing member has a groove structure.

Abstract: The present invention provides: a nucleic acid delivery controlling system in which a novel delay principle is utilized to greatly delay the nanopore passing rate of a nucleic acid strand, thereby enabling the stable analysis of a nucleotide sequence; a method for manufacturing the nucleic acid delivery controlling system; and a nucleic acid sequencing device.

Abstract: To introduce a biomolecule into a nanopore without the need to check the position of the nanopore in a thin film. In addition, displacement stability is ensured and stable acquisition of blocking signals is realized. An immobilization member 107 having a larger size than a thin film 113 with a nanopore 112 is used, and biomolecules are immobilized on the biomolecule immobilization member 107 at a density that allows at least one biomolecule 108 to enter an electric field region around the nanopore when the biomolecule immobilization member 107 has moved close to a nanopore device 101.