Patents by Inventor Rena Akahori

Rena Akahori has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10294525
    Abstract: To introduce a biomolecule into a nanopore without the need to check the position of the nanopore in a thin film. In addition, displacement stability is ensured and stable acquisition of blocking signals is realized. An immobilization member 107 having a larger size than a thin film 113 with a nanopore 112 is used, and biomolecules are immobilized on the biomolecule immobilization member 107 at a density that allows at least one biomolecule 108 to enter an electric field region around the nanopore when the biomolecule immobilization member 107 has moved close to a nanopore device 101.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: May 21, 2019
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Rena Akahori, Itaru Yanagi, Kenichi Takeda
  • Patent number: 10253362
    Abstract: The purpose of the present invention is to provide a DNA transport control device having excellent reliability and durability, and a DNA sequencing device that uses the DNA transport control device. The present invention provides a DNA transport control device having a nanopore which allows for the passage of only the DNA strand of a single molecule, and a DNA sequencing device that uses the DNA transport control device. The DNA transport control device is characterized by the following: including a base material having openings and a thin film a block copolymer formed on the base material; the thin film including microdomains that are formed as a result of self-assembly of the block copolymer and that penetrate the thin film, and a matrix surrounding the microdomains; and the nanopore being formed from one opening in the base material and a single microdomain.
    Type: Grant
    Filed: November 4, 2014
    Date of Patent: April 9, 2019
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Hiroshi Yoshida, Rena Akahori, Yasuhiko Tada, Shohei Terada, Takanobu Haga, Takashi Anazawa
  • Publication number: 20190086362
    Abstract: A traditional nanopore DNA sequencing method has a problem in that a signal analysis error may occur when a signal variation reflecting fluctuation in a base current is contained in a signal variation in a signal analysis. An electrolyte solution for biomolecule assays, containing D2O as a solvent, and/or containing an electrolyte that has Cs and Na, Na alone, Na and Li, or Li alone as a cation species in the electrolyte solution, or trishydroxyaminomethane, or a combination thereof is used in formation of a nanopore or in measurement.
    Type: Application
    Filed: March 7, 2017
    Publication date: March 21, 2019
    Applicant: Hitachi, Ltd.
    Inventors: Rena AKAHORI, Yusuke GOTO, Kazuma MATSUI, Takahide YOKOI
  • Publication number: 20180372712
    Abstract: A biomolecule measuring device includes a first liquid tank and a second liquid tank which are filled with an electrolytic solution, a nanopore device that supports a thin film having a nanopore and is provided between the first liquid tank and the second liquid tank so as to communicate between the first liquid, tank and the second liquid tank through the nanopores, and an immobilizing member that is disposed in the first liquid tank, has a size larger than that of the thin film, and to which the biomolecules are immobilized, in which at least, one of the nanopore device and the immobilizing member has a groove structure.
    Type: Application
    Filed: November 29, 2016
    Publication date: December 27, 2018
    Inventors: Rena AKAHORI, Kenichi TAKEDA, Itaru YANAGI
  • Publication number: 20170299548
    Abstract: The present invention provides: a nucleic acid delivery controlling system in which a novel delay principle is utilized to greatly delay the nanopore passing rate of a nucleic acid strand, thereby enabling the stable analysis of a nucleotide sequence; a method for manufacturing the nucleic acid delivery controlling system; and a nucleic acid sequencing device.
    Type: Application
    Filed: October 14, 2015
    Publication date: October 19, 2017
    Applicant: Hitachi High-Technologies Corporation
    Inventors: Hiroshi YOSHIDA, Rena AKAHORI, Takanobu HAGA
  • Publication number: 20170268054
    Abstract: To introduce a biomolecule into a nanopore without the need to check the position of the nanopore in a thin film. In addition, displacement stability is ensured and stable acquisition of blocking signals is realized. An immobilization member 107 having a larger size than a thin film 113 with a nanopore 112 is used, and biomolecules are immobilized on the biomolecule immobilization member 107 at a density that allows at least one biomolecule 108 to enter an electric field region around the nanopore when the biomolecule immobilization member 107 has moved close to a nanopore device 101.
    Type: Application
    Filed: October 28, 2015
    Publication date: September 21, 2017
    Inventors: Rena AKAHORI, Itaru YANAGI, Kenichi TAKEDA
  • Publication number: 20170138899
    Abstract: While an insulating film having a near-field light generating element placed thereon is being irradiated with light in an electrolytic solution, or after the film that has been irradiated with light is disposed in the electrolytic solution, a first voltage is applied between the two electrodes installed in the electrolytic solution across the film, a second voltage is then applied between the two electrodes, and a value of a current that flows between the two electrodes due to the application of the second voltage is detected. This procedure is stopped when the current value reaches or exceeds a pre-set threshold value, whereby a hole is formed at a desired location in the thin-film.
    Type: Application
    Filed: March 26, 2015
    Publication date: May 18, 2017
    Applicant: Hitachi High-Technologies Corporation
    Inventors: Naoshi ITABASHI, Sonoko MIGITAKA, Itaru YANAGI, Rena AKAHORI, Kenichi TAKEDA
  • Patent number: 9562809
    Abstract: The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 130b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a, 130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: February 7, 2017
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Satoshi Ozawa, Takashi Anazawa, Rena Akahori, Satoshi Takahashi, Takeshi Ohura, Masashi Kiguchi
  • Publication number: 20160327513
    Abstract: A pore forming method in which a pore is formed in such a way that a first voltage is applied between electrodes that are disposed with a film in an electrolytic solution therebetween; a second voltage, which is lower than the first voltage, is applied between the electrodes; a current that flows between the electrodes owing to the application of the second voltage is measured; it is judged whether a value of a current is equal to or larger than a predefined threshold; and if the value of the current is smaller than the threshold, the above sequence is repeated until a pore is formed. In this case, the second voltage is a voltage that makes the value (IPF) of the current flowing through the film practically 0. With the use of the above method, a nanopore is formed in the film simply, easily, and accurately.
    Type: Application
    Filed: December 25, 2013
    Publication date: November 10, 2016
    Inventors: Itaru YANAGI, Rena AKAHORI, Kenichi TAKEDA
  • Publication number: 20160244823
    Abstract: The purpose of the present invention is to provide a DNA transport control device having excellent reliability and durability, and a DNA sequencing device that uses the DNA transport control device. The present invention provides a DNA transport control device having a nanopore which allows for the passage of only the DNA strand of a single molecule, and a DNA sequencing device that uses the DNA transport control device. The DNA transport control device is characterized by the following: including a base material having openings and a thin film a block copolymer formed on the base material; the thin film including microdomains that are formed as a result of self-assembly of the block copolymer and that penetrate the thin film, and a matrix surrounding the microdomains; and the nanopore being formed from one opening in the base material and a single microdomain.
    Type: Application
    Filed: November 4, 2014
    Publication date: August 25, 2016
    Inventors: Hiroshi YOSHIDA, Rena AKAHORI, Yasuhiko TADA, Shohei TERADA, Takanobu HAGA, Takashi ANAZAWA
  • Publication number: 20130176563
    Abstract: The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 130b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a, 130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.
    Type: Application
    Filed: July 21, 2011
    Publication date: July 11, 2013
    Inventors: Satoshi Ozawa, Takashi Anazawa, Rena Akahori, Satoshi Takahashi, Takeshi Ohura, Masashi Kiguchi
  • Publication number: 20120312083
    Abstract: There have been the following problems with sequence analysis using multiple nanopores: trapping a sample in the nanopores is not always 100% efficient and unnecessary time is spent to measure pores in which no sample has been trapped, resulting in low measurement efficiency. To address the problems, a labeling substance is boned to a sample, and the sample to which the labeling substance has been bonded is trapped in the nanopores. An apparatus for observing the labeling substance is used to observe the labeling substance and monitor whether or not the sample has been trapped in the nanopores. Measuring only nanopores in which the sample has been trapped allows the measurement efficiency to be improved.
    Type: Application
    Filed: February 23, 2011
    Publication date: December 13, 2012
    Inventors: Rena Akahori, Takashi Anazawa, Satoshi Ozawa, Yoshiaki Yazawa, Tomoyuki Sakai, Hideyuki Noda