Abstract: The present invention provides methods for adsorbing a protein onto a conductive solid substrate, comprising the application of an external potential to the solid substrate greater than +500 mV vs. the Ag/AgCl/Cl?SAT reference electrode, in the presence of a solution comprising the protein, wherein the solution has a pH within ±2 units of the isoelectric point of the protein, and wherein the protein adsorbs to the conductive solid substrate upon application of the external potential. Some embodiments are directed to a process of protein adsorption on a solid surface mediated by an applied potential that can be affected significantly by altering a number of variables such as protein concentration, flexibility, charge, molecular weight, pH, and charge of substrate.

Abstract: The present invention provides methods for adsorbing a protein onto a conductive solid substrate, comprising the application of an external potential to the solid substrate greater than +500 mV vs. the Ag/AgCl/Cl?SAT reference electrode, in the presence of a solution comprising the protein, wherein the solution has a pH within + 2 units of the isoelectric point of the protein, and wherein the protein adsorbs to the conductive solid substrate upon application of the external potential. Some embodiments are directed to a process of protein adsorption on a solid surface mediated by an applied potential that can be affected significantly by altering a number of variables such as protein concentration, flexibility, charge, molecular weight, pH, and charge of substrate.

Abstract: A method for directing, enhancing, and accelerating mesenchymal stem cell functions using alternating electric current. Mesenchymal stem cells are preferentially directed to either osteoblast or chondrocyte lineages, but not to the adipocyte lineage. when exposed to alternating electric current.

Abstract: A method for directing, enhancing, and accelerating mesenchymal stem cell functions using alternating electric current. Mesenchymal stem cells are preferentially directed to either osteoblast or chondrocyte lineages, but not to the adipocyte lineage. when exposed to alternating electric current.

Abstract: The present invention provides a reepithelialization/wound healing implant device comprising a barrier layer and one or more polymer layers doped with agents that promote one or more processes in reepithelialization/wound healing. The implant: [a] provides a temporary mechanical/chemical barrier to species within the external environment that inhibit epithelial migration (e.g., unbalanced production/expression of granulation tissue, inhibitory proteins); [b] biodegrades at an appropriate rate while tissue remodeling is occurring; and [c] delivers active cytokines and growth factors in a choreographed pattern to promote reepithelialization.

Abstract: A device comprising a planar integrated circuit that includes an array of electrodes and at least one nanostructure, having a major axis, in electrical contact with at least one electrode. The device forms an interface between an integrated circuit platform and electro-physiologically active cells and is used in manipulate the same.

Abstract: The present invention provides a reepithelialization/wound healing implant device comprising a barrier layer and one or more polymer layers doped with agents that promote one or more processes in reepithelialization/wound healing. The implant: [a]provides a temporary mechanical/chemical barrier to species within the external environment that inhibit epithelial migration (e.g., unbalanced production/expression of granulation tissue, inhibitory proteins); [b] biodegrades at an appropriate rate while tissue remodeling is occurring; and [c] delivers active cytokines and growth factors in a choreographed pattern to promote reepithelialization.

Abstract: Exposing osteoblasts on an electrically conducting nanocomposite, which may be an orthopaedic/dental implant, to electrical stimulation enhances osteoblast proliferation thereon. The electrically conducting nanoscale material includes an electrically conducting nanoscale material and a biocompatible polymer and/or a biocompatible ceramic; carbon nanotubes may be used as the electrically conducting nanoscale material.

Abstract: Methods for enhancing osteoblast functions on a surface of an orthopaedic/dental implant and enhancing osseointegration of an orthopaedic/dental implant consist of providing an orthopaedic/dental implant comprising one or more nanostructured ceramic having a grain size of 1-100 nm or a composite of one or more adhesion-promoting polymers and one or more nanostructured ceramic having a grain size of 1-100 nm, and placing the implant in the body of an animal, where it may be exposed to osteoblast cells. Alumina, titania, or hydroxyapatite is preferred as the nanostructured ceramic.

Abstract: Peptides containing the amino acid sequence, aa1-aa2-aa3-aa4-(Gly)n, devices for implantation coated with the peptides and methods for altering cell adhesion using the peptides are disclosed. The residues aa1, aa2, and aa4 are independently Lys, Arg, Orn or, when the residue is N-terminal, Acp. The residue aa3 is Ala, Gly, Val, Leu, Ile, Ser, Thr, Cys, Met, Asn, Nle, Nva or Abu. The preferred sequence is KRSR. Peptides containing cell adhesion-related sequences can be incorporated into or coated onto substrates to enhance osteoblast adhesion.