Abstract: The invention relates to a device for electrophoretically separating molecules. The inventive device comprises: at least one plate having a top side and an underside; a first separating channel provided for receiving at least one first separating medium; at least one second channel, which is orthogonal to said first separating channel and which is provided for receiving a second separating medium; optionally comprises implements provided for filling the device with reagents, solvents, buffers, separating media and/or for loading the device with a sample to be separated, and; terminals for applying electric separating voltage to the separating channels. In the device, the first separating channel is located on the plate top side and comprises at least one first opening that leads to the plate underside, whereby the second separating channel is located on the plate underside and comprises at least one second opening.

Abstract: The invention relates to a device for electrophoretically separating molecules. The inventive device comprises: at least one plate (100) having a top side (200) and an underside (300); a first separating channel (210) provided for receiving at least one first separating medium (220); at least one second channel (310), which is orthogonal to said first separating channel and which is provided for receiving a second separating medium; optionally comprises implements provided for filling the device with reagents, solvents, buffers, separating media and/or for loading the device with a sample to be separated, and; terminals for applying electric separating voltage to the separating channels. In the device, the first separating channel is located on the plate top side and comprises at least one first opening (216) that leads to the plate underside, whereby the second separating channel is located on the plate underside and comprises at least one second opening (316).

Abstract: 3'-(2')-Amino- or thiol-modified, fluorescent dye-coupled nucleosides, nucleotides and oligonucleotides, and a process for the preparation and the use thereofThe OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatized to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of complementary strands in the presence of a template strand or of oligonucleotides and for the detection of genetic material.

Abstract: 3'-(2')-Amino- or thiol-modified, fluorescent dye-coupled nucleosides, nucleotides and oligonucleotides, and a process for the preparation and the use thereof.The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatized to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of complementary strands in the presence of a template strand or of oligonucleotides and for the detection of genetic material.

Abstract: The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatized to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of complementary strands in the presence of a template strand or of oligonucleotides and for the detection of genetic material. They have the advantage that the fluorescent label need no longer be attached to the 5' end of the oligonucleotide or to the nucleobase, and thus need not be introduced during the chemical synthesis as in labeling techniques hitherto known, while the known and conventional methods have the disadvantage that only a few polymerases can be employed for the synthesis, the acceptance of the triphosphates by the polymerases diminishes and, moreover, a large substrate excess is necessary.

Abstract: Low viscosity, aqueous impregnating resin solution with improved stability containing 30-70% by weight of solid resin which comprises:(A) 80-98% by weight of a melamine-formaldehyde precondensate and(B) 2-20% by weight of a water soluble copolymer.