Patents by Inventor Rene Marcel De Jong
Rene Marcel De Jong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230374443Abstract: A Saccharomyces yeast cell, preferably a Saccharomyces cerevisae yeast cell, comprising one or more nucleic acid sequences encoding an enzyme having NADH-dependent nitrate reductase activity and/or one or more nucleic acid sequences encoding an enzyme having NADH-dependent nitrite reductase activity. An anaerobic fermentation process for the production of a fermentation product using such Saccharomyces yeast cell and a fermentation co-product produced.Type: ApplicationFiled: October 5, 2021Publication date: November 23, 2023Inventors: Hans Marinus Charles Johannes DE BRUIJN, Phillip E. BUREMAN, Evert Tjeerd VAN RIJ, René Marcel DE JONG
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Publication number: 20230265443Abstract: The present invention is related to a novel process for production of retinyl acetate in a host cell, particularly oleaginous yeast such as e.g. Yarrowia, growing on triglyceride oils, such as e.g. vegetable oil, wherein the host cell exhibits modified lipase activity in such a way that conversion of retinol into fatty acid retinyl esters (FAREs) is reduced or abolished. Such process is especially useful in a biotechnological process for production of vitamin A.Type: ApplicationFiled: July 22, 2021Publication date: August 24, 2023Inventors: Peter Louis HOUSTON, Valmik Kanubhai VYAS, René Marcel DE JONG, Anna SYMBOR-NAGRABSKA, Ethan LAM
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Publication number: 20230250406Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide when aligned with the polypeptide according to SEQ ID NO: 1, comprises at least an amino acid substitution G414X, wherein the numbering of amino acid position(s) is/are defined with reference to SEQ ID NO: 1. The invention further relates to a process for preparing a product comprising an oil or fat comprising bringing an intermediary form of the product comprising oil or fat into contact with a polypeptide as disclosed herein and the use of a polypeptide as disclosed herein to saturated fatty acids in an oil or fat.Type: ApplicationFiled: May 17, 2019Publication date: August 10, 2023Inventors: René Marcel DE JONG, Willem BIJLEVELD, Paul Frederick MUGFORD
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Publication number: 20230227510Abstract: The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polytpeptide when measured under the same conditions.Type: ApplicationFiled: February 10, 2023Publication date: July 20, 2023Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Viktor Marius BOER, Priscilla ZWARTJENS, Jos VAN VUGT, René Marcel DE JONG
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Patent number: 11667902Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.Type: GrantFiled: December 2, 2020Date of Patent: June 6, 2023Assignee: DSM IP ASSETS B.V.Inventors: Rene Marcel De Jong, Chantal Christis, Neil Carr
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Patent number: 11608364Abstract: The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polypeptide when measured under the same conditions.Type: GrantFiled: April 29, 2019Date of Patent: March 21, 2023Assignee: DSM IP ASSETS B.V.Inventors: Johannes Gustaaf Ernst Van Leeuwen, Viktor Marius Boer, Priscilla Zwartjens, Jos Van Vugt, René Marcel De Jong
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Publication number: 20230080079Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide when aligned with the polypeptide according to SEQ ID NO: 1, comprises at least an amino acid substitution L410X and optionally one or more amino acid substitutions chosen from S365Q, S365N, L413M, G414A, G414S, G414V, G414T, V534L and V534l, wherein the 10 numbering of amino acid position(s) is/are defined with reference to SEQ ID NO: 1. The invention further relates to a process for preparing a product comprising an oil or fat comprising bringing an intermediary form of the product comprising oil or fat into contact with a polypeptide as disclosed herein and the use of a polypeptide as disclosed herein to saturated fatty acids in an oil or fat.Type: ApplicationFiled: May 17, 2019Publication date: March 16, 2023Inventors: René Marcel DE JONG, Willem BIJLEVELD, Paul Frederick MUGFORD
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Publication number: 20230049760Abstract: The present invention is related to a retinoid-producing host cell, particularly oleaginous yeast, modified such that the percentage of retinyl acetate based on the total retinoids produced by such host cell is increased during fermentation using triglyceride oils, like for example vegetable oil, as carbon source, wherein the activity of certain endogenous hydrolases or transferases involved in undesired conversions of retinol or retinol acetate is reduced or abolished. Particularly, such modified host cell might be useful in a biotechnological process for production of vitamin A.Type: ApplicationFiled: December 18, 2020Publication date: February 16, 2023Inventors: Jenna MCMAHON, Elvin Irsan KOOI, Liang WU, René Marcel DE JONG, Valmik Kanubhai VYAS, Peter Louis HOUSTON
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Publication number: 20220356503Abstract: The present invention is related to a method for increasing the trans-specificity of a beta-carotene oxidase (BCO), particularly insect BCO, to be used in the production of vitamin A aldehyde (retinal) from conversion of beta-carotene, with at least about 75 to 100% of retinal in the trans-isoform.Type: ApplicationFiled: July 15, 2020Publication date: November 10, 2022Inventors: Peter Louis HOUSTON, René Marcel DE JONG
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Patent number: 11447759Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72.Type: GrantFiled: February 12, 2020Date of Patent: September 20, 2022Assignee: DSM IP ASSETS B.V.Inventors: Alrik Pieter Los, Rene Marcel De Jong, Maaike Appeldoorn
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Patent number: 11339379Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.Type: GrantFiled: August 21, 2020Date of Patent: May 24, 2022Assignee: DSM IP ASSETS B.V.Inventors: Alrik Pieter Los, Rene Marcel De Jong, Remko Tsjibbe Winter, Ben Den Dulk
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Publication number: 20220064607Abstract: The present invention is related to production of retinyl acetate generated via enzymatic conversion of retinol, said process including the use of modified enzymes with improved activity.Type: ApplicationFiled: December 30, 2019Publication date: March 3, 2022Inventors: Peter Louis HOUSTON, René Marcel DE JONG, Valmik Kanubhai VYAS, René VERWAAL, Jenna McMAHON, Anna SYMBOR-NAGRABSKA
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Publication number: 20210238235Abstract: The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polytpeptide when measured under the same conditions.Type: ApplicationFiled: April 29, 2019Publication date: August 5, 2021Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Viktor Marius BOER, Priscilla ZWARTJENS, Jos VAN VUGT, René Marcel DE JONG
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Publication number: 20210161158Abstract: The present invention relates to a variant polypeptide having maltogenic alpha-amylase activity wherein the polypeptide comprises an amino acid sequence, which, when aligned with an amino acid sequence of SEQ ID NO: 1, comprises an amino acid substitution F188L/I, S200N, and D261G, and optionally a further amino acid substitution T288P, wherein the amino acid substitutions are determined with reference to SEQ ID NO: 1, and wherein the polypeptide has an amino acid sequence which has at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or at least 99% identity to the amino acid sequence of SEQ ID NO: 1. The invention also relates to a process for the preparation of a dough or a baked product wherein the variant polypeptide is used.Type: ApplicationFiled: April 4, 2019Publication date: June 3, 2021Inventors: Joke Anneleen PUTSEYS, Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN, René Marcel DE JONG, Jeroen GODEFROOIJ, Helma Arina STOLZE-LAGERWEIJ
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Publication number: 20210115418Abstract: The present invention relates to a variant polypeptide having a lipase activity, wherein the polypeptide may be a polypeptide that has an amino acid sequence which has at least 70% identity to a mature amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 6, wherein the polypeptide comprises an amino acid substitution at position 295 and at least one further amino acid substitution at position 113, 121, 179 and/or 284, wherein the positions are defined with reference to SEQ ID NO: 1 or SEQ ID NO: 6. The invention further relates to a process for preparing a dough wherein a polypeptide as disclosed herein is used and baked product prepared from the dough.Type: ApplicationFiled: June 18, 2019Publication date: April 22, 2021Inventors: Joke Anneleen PUTSEYS, Neil CARR, René Marcel DE JONG, Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN
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Publication number: 20210087542Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.Type: ApplicationFiled: December 2, 2020Publication date: March 25, 2021Inventors: Rene Marcel DE JONG, Chantal CHRISTIS, Neil CARR
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Patent number: 10918113Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 53, 112, 141, 178, 179, 182, 202, 203, 235, 282, 284, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.Type: GrantFiled: December 19, 2017Date of Patent: February 16, 2021Assignee: DSM IP ASSETS B.V.Inventors: Rene Marcel De Jong, Chantal Christis, Neil Carr
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Patent number: 10889807Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.Type: GrantFiled: December 19, 2017Date of Patent: January 12, 2021Assignee: DSM IP ASSETS B.V.Inventors: Rene Marcel De Jong, Chantal Christis, Neil Carr
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Publication number: 20200385692Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.Type: ApplicationFiled: August 21, 2020Publication date: December 10, 2020Inventors: Alrik Pieter LOS, Rene Marcel DE JONG, Remko Tsjibbe WINTER, Ben DEN DULK
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Patent number: 10829748Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide, which, when aligned with a polypeptide according to SEQ ID NO: 1, comprises at least one amino acid substitution resulting in Ser (S), Ala (A) or Leu (L) at position 246, Trp (W) at position 307, Leu (L) at position 345, Ile (I) at position 365, and/or Phe (F) at position 534, wherein the position is defined with reference to SEQ ID NO: 1, wherein Ala(A) at position 1 in SEQ ID NO: 1 is counted as number 1 and a method for preparing the polypeptide. The present invention further relates to a process for preparing a food product wherein a polypeptide according to the present invention is used.Type: GrantFiled: June 8, 2017Date of Patent: November 10, 2020Assignee: DSM IP ASSETS B.V.Inventors: Rene Marcel De Jong, Willem Bijleveld, Evert Tjeerd Van Rij