Patents by Inventor Rene Marcel De Jong

Rene Marcel De Jong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220356503
    Abstract: The present invention is related to a method for increasing the trans-specificity of a beta-carotene oxidase (BCO), particularly insect BCO, to be used in the production of vitamin A aldehyde (retinal) from conversion of beta-carotene, with at least about 75 to 100% of retinal in the trans-isoform.
    Type: Application
    Filed: July 15, 2020
    Publication date: November 10, 2022
    Inventors: Peter Louis HOUSTON, René Marcel DE JONG
  • Patent number: 11447759
    Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72.
    Type: Grant
    Filed: February 12, 2020
    Date of Patent: September 20, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Maaike Appeldoorn
  • Patent number: 11339379
    Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Grant
    Filed: August 21, 2020
    Date of Patent: May 24, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Remko Tsjibbe Winter, Ben Den Dulk
  • Publication number: 20220064607
    Abstract: The present invention is related to production of retinyl acetate generated via enzymatic conversion of retinol, said process including the use of modified enzymes with improved activity.
    Type: Application
    Filed: December 30, 2019
    Publication date: March 3, 2022
    Inventors: Peter Louis HOUSTON, René Marcel DE JONG, Valmik Kanubhai VYAS, René VERWAAL, Jenna McMAHON, Anna SYMBOR-NAGRABSKA
  • Publication number: 20210238235
    Abstract: The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polytpeptide when measured under the same conditions.
    Type: Application
    Filed: April 29, 2019
    Publication date: August 5, 2021
    Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Viktor Marius BOER, Priscilla ZWARTJENS, Jos VAN VUGT, René Marcel DE JONG
  • Publication number: 20210161158
    Abstract: The present invention relates to a variant polypeptide having maltogenic alpha-amylase activity wherein the polypeptide comprises an amino acid sequence, which, when aligned with an amino acid sequence of SEQ ID NO: 1, comprises an amino acid substitution F188L/I, S200N, and D261G, and optionally a further amino acid substitution T288P, wherein the amino acid substitutions are determined with reference to SEQ ID NO: 1, and wherein the polypeptide has an amino acid sequence which has at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or at least 99% identity to the amino acid sequence of SEQ ID NO: 1. The invention also relates to a process for the preparation of a dough or a baked product wherein the variant polypeptide is used.
    Type: Application
    Filed: April 4, 2019
    Publication date: June 3, 2021
    Inventors: Joke Anneleen PUTSEYS, Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN, René Marcel DE JONG, Jeroen GODEFROOIJ, Helma Arina STOLZE-LAGERWEIJ
  • Publication number: 20210115418
    Abstract: The present invention relates to a variant polypeptide having a lipase activity, wherein the polypeptide may be a polypeptide that has an amino acid sequence which has at least 70% identity to a mature amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 6, wherein the polypeptide comprises an amino acid substitution at position 295 and at least one further amino acid substitution at position 113, 121, 179 and/or 284, wherein the positions are defined with reference to SEQ ID NO: 1 or SEQ ID NO: 6. The invention further relates to a process for preparing a dough wherein a polypeptide as disclosed herein is used and baked product prepared from the dough.
    Type: Application
    Filed: June 18, 2019
    Publication date: April 22, 2021
    Inventors: Joke Anneleen PUTSEYS, Neil CARR, René Marcel DE JONG, Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN
  • Publication number: 20210087542
    Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.
    Type: Application
    Filed: December 2, 2020
    Publication date: March 25, 2021
    Inventors: Rene Marcel DE JONG, Chantal CHRISTIS, Neil CARR
  • Patent number: 10918113
    Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 53, 112, 141, 178, 179, 182, 202, 203, 235, 282, 284, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: February 16, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Rene Marcel De Jong, Chantal Christis, Neil Carr
  • Patent number: 10889807
    Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: January 12, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Rene Marcel De Jong, Chantal Christis, Neil Carr
  • Publication number: 20200385692
    Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Application
    Filed: August 21, 2020
    Publication date: December 10, 2020
    Inventors: Alrik Pieter LOS, Rene Marcel DE JONG, Remko Tsjibbe WINTER, Ben DEN DULK
  • Patent number: 10829748
    Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide, which, when aligned with a polypeptide according to SEQ ID NO: 1, comprises at least one amino acid substitution resulting in Ser (S), Ala (A) or Leu (L) at position 246, Trp (W) at position 307, Leu (L) at position 345, Ile (I) at position 365, and/or Phe (F) at position 534, wherein the position is defined with reference to SEQ ID NO: 1, wherein Ala(A) at position 1 in SEQ ID NO: 1 is counted as number 1 and a method for preparing the polypeptide. The present invention further relates to a process for preparing a food product wherein a polypeptide according to the present invention is used.
    Type: Grant
    Filed: June 8, 2017
    Date of Patent: November 10, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Rene Marcel De Jong, Willem Bijleveld, Evert Tjeerd Van Rij
  • Patent number: 10787649
    Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Grant
    Filed: July 11, 2017
    Date of Patent: September 29, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Remko Tsjibbe Winter, Ben Den Dulk
  • Publication number: 20200277644
    Abstract: The present invention is related to a novel enzymatic process for production of vitamin A aldehyde (retinal) via stereoselective conversion of beta-carotene which process includes the use of trans-selective enzymes having activity as beta-carotene oxidases (BCOs), in particular having preference for trans-retinal. 5 Said process is in particular useful for biotechnological production of vitamin A.
    Type: Application
    Filed: September 25, 2018
    Publication date: September 3, 2020
    Inventors: Nathalie BALCH, Paul BLOMQUIST, Reed DOTEN, Peter HOUSTON, Ethan LAM, Jenna MCMAHON, Joshua TRUEHEART, Celine VIAROUGE, René Marcel DE JONG
  • Publication number: 20200239925
    Abstract: The present invention is related to production of retinyl esters, such as in particular retinyl acetate, an important building block for production of vitamin A. The retinyl esters might be generated via enzymatic conversion of retinol which process includes the use of enzymes with acetyl-transferase (ATF) activity, thus acetylating retinol into retinol/retinyl acetate. Said process is particularly useful for biotechnological production of vitamin A.
    Type: Application
    Filed: September 25, 2018
    Publication date: July 30, 2020
    Inventors: Nathalie BALCH, Paul BLOMQUIST, Reed DOTEN, Peter HOUSTON, Ethan LAM, Jenna MCMAHON, Joshua TRUEHEART, Celine VIAROUGE, René Marcel DE JONG
  • Publication number: 20200190495
    Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72.
    Type: Application
    Filed: February 12, 2020
    Publication date: June 18, 2020
    Inventors: Alrik Pieter LOS, Rene Marcel DE JONG, Maaike APPELDOORN
  • Patent number: 10655115
    Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72.
    Type: Grant
    Filed: January 17, 2019
    Date of Patent: May 19, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Maaike Appeldoorn
  • Publication number: 20200140900
    Abstract: The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.
    Type: Application
    Filed: January 17, 2020
    Publication date: May 7, 2020
    Inventors: Maria Bernedina Elizabeth JONKERS, Paul KLAASSEN, Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN, Rene Marcel DE JONG
  • Patent number: 10612013
    Abstract: The invention relates to a variant polypeptide having lactase activity. The invention also relates to a nucleic acid sequence encoding such a variant polypeptide, to a nucleic acid construct comprising said nucleic acid sequence, to a recombinant expression vector comprising said nucleic acid construct and to a recombinant host cell comprising said expression vector. Further, the invention relates to a method for producing a lactase variant via use of such a host cell. Also, the invention relates to a method of producing a lactase polypeptide variant. The invention further relates to a composition comprising a lactase variant, to use of such a lactase variant or to the use of a lactase variant-containing composition in the preparation of a dairy product, to a process for the production of a dairy product and to the resulting dairy product.
    Type: Grant
    Filed: May 4, 2018
    Date of Patent: April 7, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: René Marcel De Jong, Ulrike Maria Müller, Petrus Jacobus Theodorus Dekker
  • Patent number: 10604773
    Abstract: The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.
    Type: Grant
    Filed: March 13, 2019
    Date of Patent: March 31, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Maria Bernedina Elizabeth Jonkers, Paul Klaassen, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Rene Marcel De Jong