Patents by Inventor Renuka de Silva

Renuka de Silva has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10703971
    Abstract: Alkaline phosphatase chemiluminescent substrate formulations are provided exhibiting rapid incubation periods and improved stability for use in immunoassays.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: July 7, 2020
    Assignee: Beckman Coulter, Inc.
    Inventors: Renuka De Silva, Robert A. Eickholt, Jaya S. Koti, Mark D. Sandison, David L. Schumm, Wenhua Xie
  • Publication number: 20190161675
    Abstract: Alkaline phosphatase chemiluminescent substrate formulations are provided exhibiting rapid incubation periods and improved stability for use in immunoassays.
    Type: Application
    Filed: June 30, 2017
    Publication date: May 30, 2019
    Applicant: Beckman Coulter, Inc.
    Inventors: Renuka DE SILVA, Robert A. EICKHOLT, Jaya S. KOTI, Mark D. SANDISON, David L. SCHUMM, Wenhua XIE
  • Patent number: 10036708
    Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.
    Type: Grant
    Filed: April 2, 2015
    Date of Patent: July 31, 2018
    Assignee: Beckman Coulter, Inc.
    Inventors: Hashem Akhavan-Tafti, Dean G. Binger, Ying Chen, Renuka De Silva, Terri McLernon, James Mendoza, Bruce H. Odegaard, Michael Salvati, Nir Shapir, Wenhua Xie
  • Publication number: 20150212005
    Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.
    Type: Application
    Filed: April 2, 2015
    Publication date: July 30, 2015
    Inventors: Hashem Akhavan-Tafti, Dean G. Binger, Ying Chen, Renuka De Silva, Terri McLernon, James Mendoza, Bruce H. Odegaard, Michael Salvati, Nir Shapir, Wenhua Xie
  • Patent number: 9029092
    Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.
    Type: Grant
    Filed: February 26, 2010
    Date of Patent: May 12, 2015
    Assignee: Beckman Coulter, Inc.
    Inventors: Hashem Akhavan-Tafti, Dean G. Binger, Ying Chen, Renuka De Silva, Terri McLernon, James Mendoza, Bruce H. Odegaard, Michael Salvati, Nir Shapir, Wenhua Xie
  • Patent number: 8772501
    Abstract: The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.
    Type: Grant
    Filed: January 24, 2013
    Date of Patent: July 8, 2014
    Assignee: Beckman Coulter, Inc.
    Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Guoping Wang, Robert A. Eickholt, Ravinder Gupta, Lakshmi S. Kaanumalle
  • Patent number: 8389298
    Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.
    Type: Grant
    Filed: March 19, 2010
    Date of Patent: March 5, 2013
    Assignee: Lumigen, Inc.
    Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Wenhua Xie
  • Patent number: 8367842
    Abstract: The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.
    Type: Grant
    Filed: July 15, 2010
    Date of Patent: February 5, 2013
    Assignee: Beckman Coulter, Inc.
    Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Guoping Wang, Robert A. Eickholt, Ravinder K. Gupta, Lakshmi S. Kaanumalle
  • Patent number: 8106203
    Abstract: Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
    Type: Grant
    Filed: June 2, 2009
    Date of Patent: January 31, 2012
    Assignee: Lumigen, Inc.
    Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua Xie
  • Publication number: 20100273189
    Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample, the assay method comprising forming a reaction mixture in an aqueous solution, by adding a chemiluminescent-labeled immobilized specific binding member, an activator-labeled specific binding member, a selective signal inhibiting agent, and a sample, wherein the chemiluminescent-labeled immobilized specific binding member and activator-labeled specific binding member bind to analyte present in the sample to form a binding complex, and adding to the reaction mixture a trigger solution to release a detectable chemiluminescent signal correlated to the amount of the analyte-bound binding complex present in the reaction mixture.
    Type: Application
    Filed: February 26, 2010
    Publication date: October 28, 2010
    Applicant: BECKMAN COULTER, INC.
    Inventors: Hashem AKHAVAN-TAFTI, Renuka DE SILVA, Terri MCLERNON, James MENDOZA, Michael SALVATI, Nir SHAPIR, Wenhua XIE
  • Publication number: 20100267071
    Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.
    Type: Application
    Filed: February 26, 2010
    Publication date: October 21, 2010
    Applicant: BECKMAN COULTER, INC.
    Inventors: Hashem AKHAVAN-TAFTI, Dean G. BINGER, Ying Chen, Renuka DE SILVA, Terri MCLERNON, James MENDOZA, Bruce H. ODEGAARD, Michael SALVATI, Nir SHAPIR, Wenhua XIE
  • Publication number: 20100190271
    Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.
    Type: Application
    Filed: March 19, 2010
    Publication date: July 29, 2010
    Applicant: Lumigen, Inc.
    Inventors: Hashem AKHAVAN-TAFTI, Renuka de Silva, Wenhua Xie
  • Publication number: 20100173429
    Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.
    Type: Application
    Filed: March 19, 2010
    Publication date: July 8, 2010
    Applicant: Lumigen, Inc.
    Inventors: Hashem AKHAVAN-TAFTI, Renuka De SILVA, Wenhua XIE
  • Patent number: 7682839
    Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.
    Type: Grant
    Filed: March 16, 2007
    Date of Patent: March 23, 2010
    Assignee: Lumigen, Inc.
    Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua Xie
  • Publication number: 20090246819
    Abstract: Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
    Type: Application
    Filed: June 2, 2009
    Publication date: October 1, 2009
    Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua XIE
  • Patent number: 7560556
    Abstract: Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
    Type: Grant
    Filed: March 4, 2005
    Date of Patent: July 14, 2009
    Assignee: Lumigen, Inc.
    Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua Xie
  • Publication number: 20070190526
    Abstract: Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.
    Type: Application
    Filed: February 15, 2007
    Publication date: August 16, 2007
    Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Robert A. Eickholt, Michael E. Mazelis, Wenhuas Xie, Richard S. Handley, Monica A. Bray, Michelle L. Mastronardi, Elizabeth A. O'Conner, Sarada Siripurapu
  • Publication number: 20070172878
    Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.
    Type: Application
    Filed: March 16, 2007
    Publication date: July 26, 2007
    Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Wenhua Xie
  • Patent number: 7247726
    Abstract: Novel compounds comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups are disclosed. The compounds are useful in methods and compositions for generating chemiluminescence rapidly by reaction with a peroxidase enzyme and a peroxide. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
    Type: Grant
    Filed: December 18, 2002
    Date of Patent: July 24, 2007
    Assignee: Lumigen, Inc.
    Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua Xie
  • Publication number: 20050158815
    Abstract: Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
    Type: Application
    Filed: March 4, 2005
    Publication date: July 21, 2005
    Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua Xie