Abstract: The invention is directed to a method to obtain the spatial location and sequence information of a target sequence of at least one m-RNA strand on a tissue sample comprising the steps a. providing a linear probe, containing a) a binding region capable of binding to the at least one m-RNA strand and b) an anchor sequence comprising a UMI region located between a first and a second locator regions and c) a primer region; b. hybridizing the linear probe with its binding region to the m-RNA strand; c. complementing the linear probe using the m-RNA strand as template thereby obtaining a reversed transcribed c-DNA strand d. hybridizing a locator molecule with its 3? and 5? ends to the first and second locator regions thereby creating a gap corresponding to the length of the UMI of the linear probe e.

Abstract: A method for mounting functional elements in a lens includes mounting the functional elements on a foil, applying a closed contour alignment surface of an alignment tool having a central opening surrounded by the closed contour on the foil portion opposite to the mounted function elements, applying underpressure on the central opening to maintain the foil portion with the mounted functional elements on the alignment tool, cutting a flap including the foil portion and supporting the functional elements out of the foil, positioning and aligning the flap through actuator, fixing the position of the flap against the adjacent foil surface, embedding the foil with the mounted functional elements in a predetermined distance to the front surface of a mould, and casting and curing the lens with the embedded foil.

Abstract: A method for mounting functional elements in a lens includes mounting the functional elements on a foil, applying a closed contour alignment surface of an alignment tool having a central opening surrounded by the closed contour on the foil portion opposite to the mounted function elements, applying underpressure on the central opening to maintain the foil portion with the mounted functional elements on the alignment tool, cutting a flap including the foil portion and supporting the functional elements out of the foil, positioning and aligning the flap through actuator, fixing the position of the flap against the adjacent foil surface, embedding the foil with the mounted functional elements in a predetermined distance to the front surface of a mould, and casting and curing the lens with the embedded foil.

Abstract: This invention relates in general to methods of sequencing multiple distinct and separate polynucleotide fragments and regions in a sequential order, such as on a flow cell surface. The invention provides methods that solve prior art problems with regard to sequential sequencing, and that provide advantages including low cost, shorter turn-around-time, high efficiency, and easy implementation.

Abstract: A reactor for direct utilization of external radiation heat includes a containment can having a longitudinal axis, an external driving mechanism for controllable rotating the containment can and a cavity having a cylindrical shape. The cavity is partially lined with an insulation layer and disposed in the containment can. The cavity has a gas inlet, a gas outlet and an aperture for allowing to insert the external radiation heat into the cavity. A feeder mechanism is moveable along the longitudinal axis into and out of the cavity for supplying the material subject to the thermal or thermo-chemical material process. The gas outlet is formed as a ring channel tube having an inner and an outer surface, wherein the feeder mechanism is at least partially hosted within a tube cavity defined by the inner surface. Cooling means are associated with the inner and/or outer surface.

Abstract: A reactor for direct utilization of external radiation heat includes a containment can having a longitudinal axis, an external driving mechanism for controllable rotating the containment can and a cavity having a cylindrical shape. The cavity is partially lined with an insulation layer and disposed in the containment can. The cavity has a gas inlet, a gas outlet and an aperture for allowing to insert the external radiation heat into the cavity. A feeder mechanism is moveable along the longitudinal axis into and out of the cavity for supplying the material subject to the thermal or thermo-chemical material process. The gas outlet is formed as a ring channel tube having an inner and an outer surface, wherein the feeder mechanism is at least partially hosted within a tube cavity defined by the inner surface. Cooling means are associated with the inner and/or outer surface.