Abstract: A composition for topical application of a botulinum toxin (including botulinum toxin derivatives) comprises a botulinum toxin and a carrier comprising a polymeric backbone comprising a long-chain polypeptide or nonpeptidyl polymer having attached positively charged branching or “efficiency” groups. The invention also relates to methods for reducing muscle paralysis and other conditions that may be treated with a botulinum toxin, particularly paralysis of subcutaneous, and most particularly, facial, muscles, by topically applying an effective amount of the botulinum toxin and carrier, in conjunction, to the subject's skin or epithelium. Kits for administration are also described.

Abstract: This invention provides devices, methods, and assessment scales for assessing the severity of wrinkles. Wrinkle assessment using the devices, methods, and assessment scales of the invention can be performed in a clinical setting for the purpose of simply characterizing a wrinkle, or as part of a treatment regimen that varies according to the severity of the wrinkle. Thus, this invention also provides methods of reducing the appearance of wrinkles, and kits comprising for evaluating and treating wrinkles.