Abstract: A single-piece hybrid droplet generator and nozzle component for serial crystallography. The single-piece hybrid droplet generator component including an internally-formed droplet-generation channel, an internally-formed sample channel, a nozzle, and a pair of electrode chambers. The droplet-generation channel extends from a first fluid inlet opening to the nozzle. The sample channel extends from a second fluid inlet opening to the droplet-generation channel and joins the droplet-generation channel at a junction. The nozzle is configured to eject a stream of segmented aqueous droplets in a carrier fluid from the droplet-generation channel through a nozzle opening of the single-piece component. The pair of electrode chambers are positioned adjacent to the droplet-generation channel near the junction between the droplet-generation channel and the sample channel.

Abstract: A single-piece hybrid droplet generator and nozzle component for serial crystallography. The single-piece hybrid droplet generator component including an internally-formed droplet-generation channel, an internally-formed sample channel, a nozzle, and a pair of electrode chambers. The droplet-generation channel extends from a first fluid inlet opening to the nozzle. The sample channel extends from a second fluid inlet opening to the droplet-generation channel and joins the droplet-generation channel at a junction. The nozzle is configured to eject a stream of segmented aqueous droplets in a carrier fluid from the droplet-generation channel through a nozzle opening of the single-piece component. The pair of electrode chambers are positioned adjacent to the droplet-generation channel near the junction between the droplet-generation channel and the sample channel.

Abstract: A microfluidic device for use in a serial crystallography apparatus includes a modular 3D-printed nozzle having an inlet, an outlet, and a first snap engagement feature. The microfluidic device further includes a modular 3D-printed fiber holder having an outlet and a second snap engagement feature. The first snap engagement feature is configured to engage the second snap engagement feature to removably couple the nozzle to the fiber holder. The outlet of the fiber holder is aligned with the inlet of the nozzle when the first snap engagement feature is coupled to the second snap engagement feature.

Abstract: Systems and methods for performing serial crystallography by providing an aqueous suspension of a crystal sample to a T-junction at a first flow rate and providing an immiscible oil fluid to the T-junction at a second flow rate. The aqueous suspension and the oil are combined at the T-junction to produce a co-flow output fluid including a parallel co-flow of the aqueous suspension and the oil in the same output channel. The co-flow output fluid is ejected as a jet stream through a nozzle and the sample flow rate in the crystal sample in the jet stream is adjusted by adjusting the first flow rate of the aqueous suspension and the second flow rate of the oil. By combining the aqueous sample and the oil in this manner, the output of the jet stream can be regulated for compatibility with X-ray free electron laser serial crystallography.

Abstract: A microfluidic device for use in a serial crystallography apparatus includes a nozzle having an inlet, an outlet, and a first snap engagement feature. The microfluidic device further includes a fiber holder having an outlet and a second snap engagement feature. The first snap engagement feature is configured to engage the second snap engagement feature to removably couple the nozzle to the fiber holder. The outlet of the fiber holder is aligned with the inlet of the nozzle when the first snap engagement feature is coupled to the second snap engagement feature.

Abstract: A nozzle includes a chamber, a first channel formed in the nozzle, and a first passageway extending between the first channel and the chamber. The first passageway defines a first passageway axis. The nozzle also includes a second channel formed in the nozzle and a second passageway extending between the second channel and the chamber. The second passageway defines a second passageway axis, and the first passageway axis is not concentric with the second passageway axis.

Abstract: Systems and methods for performing serial crystallography by providing an aqueous suspension of a crystal sample to a T-junction at a first flow rate and providing an immiscible oil fluid to the T-junction at a second flow rate. The aqueous suspension and the oil are combined at the T-junction to produce a co-flow output fluid including a parallel co-flow of the aqueous suspension and the oil in the same output channel. The co-flow output fluid is ejected as a jet stream through a nozzle and the sample flow rate in the crystal sample in the jet stream is adjusted by adjusting the first flow rate of the aqueous suspension and the second flow rate of the oil. By combining the aqueous sample and the oil in this manner, the output of the jet stream can be regulated for compatibility with X-ray free electron laser serial crystallography.

Abstract: A single-piece hybrid droplet generator and nozzle component for serial crystallography. The single-piece hybrid droplet generator component including an internally-formed droplet-generation channel, an internally-formed sample channel, a nozzle, and a pair of electrode chambers. The droplet-generation channel extends from a first fluid inlet opening to the nozzle. The sample channel extends from a second fluid inlet opening to the droplet-generation channel and joins the droplet-generation channel at a junction. The nozzle is configured to eject a stream of segmented aqueous droplets in a carrier fluid from the droplet-generation channel through a nozzle opening of the single-piece component. The pair of electrode chambers are positioned adjacent to the droplet-generation channel near the junction between the droplet-generation channel and the sample channel.

Abstract: Exemplary embodiments can maximize long-term value in a machine learning system. The system may employ an offline training process and an online training process. In the offline training process, an initial policy is learned to provide a warm start to the online training process. In the online training process, the system applies concurrent reinforcement learning across multiple environments, with the goal of learning efficient policies in real time from in-flight user data in one environment, and applying the learned policies to other environments. With the combination of offline training and online training, the system is able to improve initial performance through the warm start, while adapting to a changing context through concurrent reinforcement learning.

Abstract: Exemplary embodiments can maximize long-term value in a machine learning system. The system may employ an offline training process and an online training process. In the offline training process, an initial policy is learned to provide a warm start to the online training process. In the online training process, the system applies concurrent reinforcement learning across multiple environments, with the goal of learning efficient policies in real time from in-flight user data in one environment, and applying the learned policies to other environments. With the combination of offline training and online training, the system is able to improve initial performance through the warm start, while adapting to a changing context through concurrent reinforcement learning.

Abstract: This application discloses natural product extracts and compounds from an aerial root of a Ficus plant, such as Ficus benghalensis. The application also discloses the use of natural product extracts and compounds from Ficus plants for increasing hair growth and decreasing hair loss. Methods of producing the extracts and isolating the compounds are further disclosed.

Abstract: This application discloses natural product extracts and compounds from an aerial root of a Ficus plant, such as Ficus benghalensis. The application also discloses the use of natural product extracts and compounds from Ficus plants for increasing hair growth and decreasing hair loss. Methods of producing the extracts and isolating the compounds are further disclosed.

Abstract: This application discloses natural product extracts and compounds from an aerial root of a Ficus plant, such as Ficus benghalensis. The application also discloses the use of natural product extracts and compounds from Ficus plants for increasing hair growth and decreasing hair loss. Synthetic formulations comprising various compounds identified in the natural product extracts and methods of use thereof are further disclosed.

Abstract: The invention provides compositions and methods for increasing hair growth and decreasing hair loss. In one embodiment, the compositions comprise a plurality of hair growth agents. Optionally, the hair growth agents are selected from the group consisting of: IGF-1, FGF-2, FGF-10, PDGF-AA, Wnt-3a, noggin, ephrin-A3, sonic hedgehog (SHH), BMP-6 and hypoxanthine.

Abstract: The application describes a method of generating a hair follicle, comprising administering a composition comprising epidermal matrix cells, dermal papilla cells, dermal sheath cells and outer root sheath cells to the scalp of a mammal. The composition may optionally be administered with a hair growth agent and/or a carrier. The application also describes a composition comprising epidermal matrix cells, dermal papilla cells, dermal sheath cells and outer root sheath cells and the use of the composition to generate hair follicles.