Abstract: There is disclosed a nanopore support structure comprising a wall layer comprising walls defining a plurality of wells, and overhangs extending from the walls across each of the wells, the overhang defining an aperture configured to support a membrane suitable for insertion of a nanopore. There is further disclosed a nanopore sensing device comprising a nanopore support structure, and methods of manufacturing the nanopore support structure and the nanopore sensing device.

Abstract: The invention relates to a method of forming a sensing device for supporting a plurality of nanopores upon an array of wells. The method involves providing a substrate, said substrate having a surface having an array of electrodes located thereon for connecting to or for configuring upon an electronic circuit. Separately, a well array structure is provided, which has an array of walls defining through-holes for defining wells. The substrate and well array structures are aligning said array of electrodes define, at least in part, a portion of the bases of respective wells at the bottom of the through holes. The resulting sensing device overcomes problems with known sensing devices by employing a substrate and/or well array structure, or hybrid thereof, that employs alternative materials or manufacturing processes.