Abstract: A protected follicular aspiration device containing a tube that protects the guide line providing enhanced sealing of the system, said device also having a sealing bung arrangement that forms a foam-retaining chamber preventing foam from entering the vacuum pump and a conical coupling between the guide line with the needle cannula and the sealing bung. The follicular aspiration device is intended for collecting follicles during an in vitro fertilization process, showing improvements in protecting and minimizing exposure of the collected material.