Abstract: Provided herein are methods of improving solubilization, extraction, and isolation of recombinant spider silk proteins with a salt and alcohol buffer. Provided herein are methods of solubilizing a recombinant spider silk protein from a host cell, comprising: providing a cell culture comprising a host cell, wherein the host cell expresses a recombinant spider silk protein; collecting an insoluble portion derived from the cell culture, wherein the insoluble portion comprises the recombinant spider silk protein; adding the insoluble portion of the host cell to a solution comprising a salt and an alcohol, thereby solubilizing the recombinant spider silk protein in the solution.

Abstract: A computer implemented method of employment includes inputting a plurality of job related data sets derived from a plurality of sources of job related data into a database stored in a computer system. Job search criteria is collected from an end user. The job search criteria includes at least a description of a new job, a title of the new job and a compensation level of the new job. The job related data sets and the job search criteria are normalized into a consistent format. A computer implemented algorithm is utilized to compare the job search criteria to job related data sets of available jobs or job candidates in the database. The algorithm is utilized to rank the available jobs or job candidates in order of most closely meeting the entirety of the job search criteria based on the comparison.

Abstract: An improved liquid dispenser that is adapted to fit into a refrigerator and that is more user friendly is disclosed. The liquid dispenser includes a hollow body, a top, a bottom, and at least one wall therebetween. The body creates a receptacle for a liquid. The top includes an inlet for the receptacle and the at least one wall includes an outlet for the receptacle. A handle located near a central axis of the body is adapted to carry the liquid dispenser. The liquid dispenser may be stacked vertically with similar configured dispensers.

Abstract: An improved liquid dispenser that is adapted to fit into a refrigerator and that is more user friendly is disclosed. The liquid dispenser includes a hollow body, a top, a bottom, and at least one wall therebetween. The body creates a receptacle for a liquid. The top includes an inlet for the receptacle and the at least one wall includes an outlet for the receptacle. A handle located near a central axis of the body is adapted to carry the liquid dispenser. The liquid dispenser may be stacked vertically with similar configured dispensers.