Abstract: A flowmeter for detecting gas flow rates in a pipe includes a container configured to be attached to the pipe having a channel through which the gas flows, and a plurality of recesses that extend through the container and a plurality of housings, each recess having a housing. The flowmeter includes a plurality of transducers, with one transducer of the plurality of transducers disposed in each housing in each recess, the transducers transmitting ultrasonic signals into and receiving ultrasonic signals from the channel. The flowmeter includes acoustic isolators which acoustically isolate the housings from the container. The flowmeter includes a controller in electrical communication with the plurality of transducers which determines the gas flow rate through the channel by measuring transit times of signals transmitted by and received by the transducers. A method.

Abstract: An ultrasonic transducer apparatus is provided. In one embodiment, the apparatus includes an outer housing, an inner housing disposed within the outer housing, and an ultrasonic transducer disposed within the inner housing. The outer housing has an aperture that enables pressurized fluid to enter the outer housing while allowing the outer housing to acoustically isolate the inner housing and the ultrasonic transducer from an additional component when the outer housing is connected to the additional component. Additional systems, devices, and methods are also disclosed.

Abstract: An ultrasonic transducer apparatus is provided. In one embodiment, the apparatus includes an outer housing, an inner housing disposed within the outer housing, and an ultrasonic transducer disposed within the inner housing. The outer housing has an aperture that enables pressurized fluid to enter the outer housing while allowing the outer housing to acoustically isolate the inner housing and the ultrasonic transducer from an additional component when the outer housing is connected to the additional component. Additional systems, devices, and methods are also disclosed.

Abstract: An ultrasonic transducer apparatus is provided. In one embodiment, the apparatus includes an outer housing, an inner housing disposed within the outer housing, and an ultrasonic transducer disposed within the inner housing. The outer housing has an aperture that enables pressurized fluid to enter the outer housing while allowing the outer housing to acoustically isolate the inner housing and the ultrasonic transducer from an additional component when the outer housing is connected to the additional component. Additional systems, devices, and methods are also disclosed.

Abstract: A flowmeter for detecting gas flow rates in a pipe includes a container configured to be attached to the pipe having a channel through which the gas flows, and a plurality of recesses that extend through the container and a plurality of housings. The flowmeter includes a plurality of transducers, with one transducer of the plurality of transducers disposed in each recess. The transducers transmit ultrasonic signals into and receive ultrasonic signals from the channel. The flowmeter includes a controller in electrical communication with the plurality of transducers which determines the gas flow rate through the channel by measuring transit times of signals transmitted by and received by the transducers. A housing for an ultrasonic transducer for a flowmeter which is inserted into a recess of a container that acoustically isolates the housing from the container. A method for detecting gas flow rates in a pipe.

Abstract: A flowmeter for detecting gas flow rates in a pipe including a container configured to be attached to the pipe having a channel through which the gas flows, and a plurality of recesses that extend through the container and a plurality of housings. Each recess having one housing of the plurality of housings. Each housing having a pressure containing window. The flowmeter includes a plurality of transducers, with one transducer of the plurality of transducers disposed in each housing in each recess. The transducers transmitting ultrasonic signals through the windows into and receiving ultrasonic signals through the windows from the channel. The flowmeter includes acoustic isolators disposed about the housing along the housing's length and between the housings and the container which acoustically isolate the housings from the container.

Abstract: An ultrasonic transducer apparatus is provided. In one embodiment, the apparatus includes an outer housing, an inner housing disposed within the outer housing, and an ultrasonic transducer disposed within the inner housing. The outer housing has an aperture that enables pressurized fluid to enter the outer housing while allowing the outer housing to acoustically isolate the inner housing and the ultrasonic transducer from an additional component when the outer housing is connected to the additional component. Additional systems, devices, and methods are also disclosed.

Abstract: This invention provides a system and method for an assay used in determining appropriate immunosuppressant levels relative to organ transplant in which PBMC is separated from whole blood by Ficoll®. An aliquot of PBMC is used for phenotyping of cells. CD4, CD8, memory and naïve subsets, B-cells regulatory T-cells and other cell markers (e.g. CD31) are examined. After an aliquot of PBMC is taken, CD4 cells are isolated. DNA is isolated from the cells. CD4 cells can be used for TREC at the defined time points. The TREC assay can be performed via a validated protocol. TREC levels are then measured using a quantitative RT-PCR for single jointed TREC. Alternatively, or additionally, TREC-correlated cell markers (e.g. CD31) can be analyzed. Approximately 100,000 cells, or 2 micrograms, of DNA are desired for TREC analysis. Normal control cells are run in parallel. A kit, including instructions and various components can be provided.

Abstract: A flowmeter for detecting gas flow rates in a pipe includes a container configured to be attached to the pipe having a channel through which the gas flows, and a plurality of recesses that extend through the container and a plurality of housings. The flowmeter includes a plurality of transducers, with one transducer of the plurality of transducers disposed in each recess. The transducers transmit ultrasonic signals into and receive ultrasonic signals from the channel. The flowmeter includes a controller in electrical communication with the plurality of transducers which determines the gas flow rate through the channel by measuring transit times of signals transmitted by and received by the transducers. A housing for an ultrasonic transducer for a flowmeter which is inserted into a recess of a container that acoustically isolates the housing from the container. A method for detecting gas flow rates in a pipe.

Abstract: A flowmeter for detecting gas flow rates in a pipe includes a container configured to be attached to the pipe having a channel through which the gas flows, and a plurality of recesses. Each recess has a window made of metal. The flowmeter includes a plurality of transducers, with one transducer of the plurality of transducers disposed in each recess. The flowmeter includes a controller which determines the gas flow rate through the channel.

Abstract: A flowmeter for detecting gas flow rates in a pipe includes a container configured to be attached to the pipe having a channel through which the gas flows, and a plurality of recesses that extend through the container and a plurality of housings. Each recess has a housing which contains pressure in the channel. Each housing has a window that is in acoustic communication with the channel. The flowmeter includes a plurality of transducers, with one transducer of the plurality of transducers disposed in each recess. The transducers transmit ultrasonic signals into and receive ultrasonic signals from the channel through the window in the housing in which a transducer is disposed. The flowmeter includes a controller in electrical communication with the plurality of transducers which determines the gas flow rate through the channel by measuring transit times of signals transmitted by and received by the transducers.

Abstract: A flowmeter for detecting gas flow rates in a pipe. The flowmeter includes a container configured to be attached to the pipe having a channel through which the gas flows, and a plurality of recesses. Each recess has a window made of metal that is in acoustic communication with the channel. The flowmeter includes a plurality of transducers, with one transducer of the plurality of transducers disposed in each recess. The transducers transmit ultrasonic signals into and receive ultrasonic signals from the channel through the window in the housing recess in which a transducer is disposed. The flowmeter includes a controller in electrical communication with the plurality of transducers which determines the gas flow rate through the channel from signals received from the transducers. A housing for an ultrasonic transducer for a flowmeter which is inserted into a recess of a container. A method for detecting gas flow rates in a pipe. A transducer for an ultrasonic flowmeter.

Abstract: A flowmeter for detecting gas flow rates in a pipe includes a container configured to be attached to the pipe having a channel through which the gas flows, and a plurality of recesses that extend through the container and a plurality of housings. Each recess has a housing which contains pressure in the channel. Each housing has a window that is in acoustic communication with the channel. The flowmeter includes a plurality of transducers, with one transducer of the plurality of transducers disposed in each recess. The transducers transmit ultrasonic signals into and receive ultrasonic signals from the channel through the window in the housing in which a transducer is disposed. The flowmeter includes a controller in electrical communication with the plurality of transducers which determines the gas flow rate through the channel by measuring transit times of signals transmitted by and received by the transducers.

Abstract: This invention provides a system and method for an assay used in determining appropriate immunosuppressant levels relative to organ transplant in which PBMC is separated from whole blood by Ficoll®. An aliquot of PBMC is used for phenotyping of cells. CD4, CD8, memory and naïve subsets, B-cells regulatory T-cells and other cell markers (e.g. CD31) are examined. After an aliquot of PBMC is taken, CD4 cells are isolated. DNA is isolated from the cells. CD4 cells can be used for TREC at the defined time points. The TREC assay can be performed via a validated protocol. TREC levels are then measured using a quantitative RT-PCR for single jointed TREC. Alternatively, or additionally, TREC-correlated cell markers (e.g. CD31) can be analyzed. Approximately 100,000 cells, or 2 micrograms, of DNA are desired for TREC analysis. Normal control cells are run in parallel. A kit for performing the assay, including instructions and various components can be provided for practitioners.