Abstract: The invention relates to a method for screening target-ligand interactions while using a chemical library of ligands, to the chemical library of ligands as such, to a method for producing the chemical library, as well as to the use of the chemical library for developing active agents and for developing molecular sensors. The screening method comprises the following steps: (a) measuring at least one fluorescence property of a locally addressable chemical library of ligands that is immobilized on a solid phase, whereby a molecular fluorescence sensor is bound to each ligand; (b) adding the target, and (c) measuring the same fluorescence property/properties of the chemical library as described in step (a).