Patents by Inventor Richard B. Fair

Richard B. Fair has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190324026
    Abstract: A method comprising effecting a change in a shape of a droplet, wherein the droplet is disposed over a substrate in sensing proximity to a sensor and the droplet has a starting surface area exposed to the sensor; and producing an expanded surface area of the droplet in the sensing proximity exposed to the sensor, wherein the expanded surface area exposed to the sensor is greater than the starting surface area exposed to the sensor.
    Type: Application
    Filed: June 27, 2019
    Publication date: October 24, 2019
    Applicants: Advanced Liquid Logic, Inc., Duke University
    Inventors: Ramakrishna Sista, Vamsee K. Pamula, Michael G. Pollack, Vijay Srinivasan, Allen E. Eckhardt, Richard B. Fair
  • Patent number: 10379112
    Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance.
    Type: Grant
    Filed: May 22, 2015
    Date of Patent: August 13, 2019
    Assignees: Advanced Liquid Logic, Inc., Duke University
    Inventors: Ramakrishna Sista, Vamsee K. Pamula, Michael G. Pollack, Vijay Srinivasan, Allen E. Eckhardt, Richard B. Fair
  • Patent number: 10022719
    Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.
    Type: Grant
    Filed: November 9, 2015
    Date of Patent: July 17, 2018
    Assignee: DUKE UNIVERSITY
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair
  • Patent number: 9638662
    Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: May 2, 2017
    Assignee: DUKE UNIVERSITY
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair
  • Patent number: 9476856
    Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive
    Type: Grant
    Filed: May 9, 2013
    Date of Patent: October 25, 2016
    Assignees: ADVANCED LIQUID LOGIC, INC., DUKE UNIVERSITY
    Inventors: Vamsee K. Pamula, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair
  • Publication number: 20160231268
    Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
    Type: Application
    Filed: April 14, 2016
    Publication date: August 11, 2016
    Applicants: ADVANCED LIQUID LOGIC, INC., Duke University
    Inventors: Vamsee Pamula, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair
  • Patent number: 9395361
    Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
    Type: Grant
    Filed: June 5, 2015
    Date of Patent: July 19, 2016
    Assignees: ADVANCED LIQUID LOGIC, INC., DUKE UNIVERSITY
    Inventors: Vamsee K. Pamula, Michael G. Pollack, Vijay Srinivasan, Richard B. Fair
  • Publication number: 20160114320
    Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.
    Type: Application
    Filed: November 9, 2015
    Publication date: April 28, 2016
    Applicant: DUKE UNIVERSITY
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair
  • Patent number: 9243282
    Abstract: The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.
    Type: Grant
    Filed: May 14, 2012
    Date of Patent: January 26, 2016
    Assignees: ADVANCED LIQUID LOGIC, INC, DUKE UNIVERSITY, THE BOARD OF TRUSTEES OF THE LELAND STANDFORD JUNIOR UNIVERSITY
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair, Peter B. Griffin
  • Publication number: 20150336098
    Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
    Type: Application
    Filed: August 4, 2015
    Publication date: November 26, 2015
    Applicant: Duke University
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair
  • Patent number: 9180450
    Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.
    Type: Grant
    Filed: July 30, 2014
    Date of Patent: November 10, 2015
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair
  • Publication number: 20150314293
    Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance.
    Type: Application
    Filed: May 22, 2015
    Publication date: November 5, 2015
    Applicants: DUKE UNIVERSITY, ADVANCED LIQUID LOGIC, INC.
    Inventors: Ramakrishna Sista, Vamsee K. Pamula, Michael G. Pollack, Vijay Srinivasan, Allen E. Eckhardt, Richard B. Fair
  • Patent number: 9110017
    Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: August 18, 2015
    Assignee: DUKE UNIVERSITY
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair
  • Publication number: 20150174577
    Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.
    Type: Application
    Filed: March 5, 2015
    Publication date: June 25, 2015
    Applicants: Advanced Liquid Logic, Inc., Duke University
    Inventors: Vijay Srinivasan, Michael G. Pollack, Philip Y. Paik, Vamsee K. Pamula, Richard B. Fair
  • Patent number: 9046514
    Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance.
    Type: Grant
    Filed: February 11, 2008
    Date of Patent: June 2, 2015
    Assignees: ADVANCED LIQUID LOGIC, INC., DUKE UNIVERSITY
    Inventors: Ramakrishna Sista, Vamsee K. Pamula, Michael G. Pollack, Vijay Srinivasan, Allen E. Eckhardt, Richard B. Fair
  • Publication number: 20150148238
    Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 28, 2015
    Applicants: ADVANCED LIQUID LOGIC, INC., Duke University
    Inventors: Vamsee Pamula, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair
  • Patent number: 8980198
    Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.
    Type: Grant
    Filed: December 15, 2006
    Date of Patent: March 17, 2015
    Assignees: Advanced Liquid Logic, Inc., Duke University
    Inventors: Vijay Srinivasan, Michael G. Pollack, Philip Y. Paik, Vamsee K. Pamula, Richard B. Fair
  • Publication number: 20150060284
    Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
    Type: Application
    Filed: November 11, 2014
    Publication date: March 5, 2015
    Applicant: DUKE UNIVERSITY
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair
  • Patent number: 8951721
    Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
    Type: Grant
    Filed: November 10, 2009
    Date of Patent: February 10, 2015
    Assignees: Advanced Liquid Logic, Inc., Duke University
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Vijay Srinivasan, Richard B. Fair
  • Patent number: 8906627
    Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
    Type: Grant
    Filed: January 9, 2013
    Date of Patent: December 9, 2014
    Assignee: Duke University
    Inventors: Michael G Pollack, Vamsee K Pamula, Richard B Fair