Patents by Inventor Richard Benjamin Ciccarelli

Richard Benjamin Ciccarelli has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of detecting foreign DNA integrated in eukaryotic chromosomes
    Patent number: 6168918
    Abstract: Methods of detecting the presence of a plasmid DNA sequence integrated in a chromosomal DNA molecule of a eukaryotic cell in a sample that contains chromosomal DNA molecules of eukaryotic cells and free plasmid DNA molecules are disclosed. According to the invention, chromosomal DNA of eukaryotic cells which are free of deoxyadenosine methyltransferase, and free plasmid DNA molecules which are produced in cells that contain deoxyadenosine methyltransferase and which have a DpnI site, are digested with one or more restriction enzymes that cleave plasmid DNA sequences integrated in the chromosomal DNA and plasmid DNA molecules to produce DNA digestion segments that are then fractionated to produce a plurality of fractions. The DNA digestion segments in each fraction is digested with DpnI and plasmid DNA sequences are amplified using sets of primers that flank a DpnI site in the plasmid DNA sequence.
    Type: Grant
    Filed: January 31, 1996
    Date of Patent: January 2, 2001
    Assignee: American Home Products Corp.
    Inventors: C. Satishchandran, Richard Benjamin Ciccarelli, Catherine Julia Pachuk