Abstract: A method of installing a plurality of nut and bolt fasteners upon a structure is disclosed. The method includes placing a jig around the nut of a first fastener and the nut of a second fastener, securing the jig to the first fastener, and then using a power tool to tighten the nut of the second fastener to a target torque. The step of tightening the nut comprises engaging a socket portion of the power tool with the nut, securing the power tool to the jig such that a handle portion of the power tool is prevented from rotating about an axis of rotation of a socket portion of the power tool when the nut is being rotated by the socket portion. A jig, jig fasteners for securing a jig to a fastener, and a fastening head for a power tool are disclosed.

Abstract: Antisense oligomers complementary to a selected target site in the human dystrophin gene to induce exon 51 skipping are described.

Abstract: A method of installing a plurality of nut and bolt fasteners upon a structure is disclosed. The method includes placing a jig around the nut of a first fastener and the nut of a second fastener, securing the jig to the first fastener, and then using a power tool to tighten the nut of the second fastener to a target torque. The step of tightening the nut comprises engaging a socket portion of the power tool with the nut, securing the power tool to the jig such that a handle portion of the power tool is prevented from rotating about an axis of rotation of a socket portion of the power tool when the nut is being rotated by the socket portion. A jig, jig fasteners for securing a jig to a fastener, and a fastening head for a power tool are disclosed.

Abstract: A method of forming a rib-spar joint during assembly of an aircraft wing, the method comprising: providing a spar with a fixedly attached rib post, the rib post having a first coordination feature; providing a rib having a web with a first tooling feature; providing a tooling component having a second tooling feature for cooperating with the first tooling feature, a second coordination feature for cooperating with the first coordination feature, and a plurality of drill bushings; fixedly attaching the tooling component to the rib web by aligning the first and second tooling features; aligning the rib with the spar by aligning the first and second coordination features; drilling fastener holes in the rib and the rib post off the drill bushings of the tooling component; removing the tooling component from the rib web; and fastening the rib to the rib post using the fastener holes to form a rib-spar joint. The method may be applied to form front and/or rear rib-spar joints.

Abstract: A method of forming a rib-spar joint during assembly of an aircraft wing, the method comprising: providing a spar with a fixedly attached rib post, the rib post having a first coordination feature; providing a rib having a web with a first tooling feature; providing a tooling component having a second tooling feature for cooperating with the first tooling feature, a second coordination feature for cooperating with the first coordination feature, and a plurality of drill bushings; fixedly attaching the tooling component to the rib web by aligning the first and second tooling features; aligning the rib with the spar by aligning the first and second coordination features; drilling fastener holes in the rib and the rib post off the drill bushings of the tooling component; removing the tooling component from the rib web; and fastening the rib to the rib post using the fastener holes to form a rib-spar joint. The method may be applied to form front and/or rear rib-spar joints.

Abstract: The invention provides antisense antiviral compounds and methods of their use and production in inhibition of growth of viruses of the Picornaviridae family and in the treatment of a viral infection. The compounds are particularly useful in the treatment of Enterovirus and/or Rhinovirus infection in a mammal. The antisense antiviral compounds are substantially uncharged, morpholino oligonucleotides have a sequence of 12-40 subunits, including at least 12 subunits having a targeting sequence that is complementary to a region associated with viral RNA sequences within a 32 nucleotide region of the viral 5? untranslated region identified by SEQ ID NO:7.

Abstract: The invention provides antisense antiviral compounds and methods of their use in inhibition of growth of human immunodeficiency virus-1 (HOV-1), as in treatment of a viral infection. The antisense antiviral compounds have morpholino subunits linked by uncharged phosphorodiamidate linkages interspersed with cationic phosphorodiamidate linkages. An exemplary embodiment of the invention provides an antisense compound directed to the HIV Vif gene, causing the production of defective HIV- 1 virions in an infected individual.

Abstract: A method and oligonucleotide compound for inhibiting replication of a nidovirus in virus-infected animal cells are disclosed. The compound (i) has a nuclease-resistant backbone, (ii) is capable of uptake by the infected cells, (iii) contains between 8-25 nucleotide bases, and (iv) has a sequence capable of disrupting base pairing between the transcriptional regulatory sequences in the 5? leader region of the positive-strand viral genome and negative-strand 3? subgenomic region. In practicing the method, infected cells are exposed to the compound in an amount effective to inhibit viral replication.

Abstract: A method and composition for of treating cancer, and in particular, for arresting the progression of a solid or primary cancer to a more invasive, metastatic state, are disclosed. The composition includes a substantially uncharged antisense compound (i) having a nuclease-resistant backbone, (ii) capable of uptake by target cancer cells in the subject, (iii) containing between 10-40 nucleotide bases, and (iv) having a base sequence effective to hybridize to a region of processed or preprocessed human SNAIL RNA transcript. The compound, when administered to the subject, is effective to form within target cancer cells in the subject, a base-paired heteroduplex structure composed of human SNAIL RNA transcript and the oligonucleotide compound, where this structure is characterized by a Tm of dissociation of at least 45° C.

Abstract: A method and oligonucleotide compound for inhibiting replication of a nidovirus in virus-infected animal cells are disclosed. The compound (i) has a nuclease-resistant backbone, (ii) is capable of uptake by the infected cells, (iii) contains between 8-25 nucleotide bases, and (iv) has a sequence capable of disrupting base pairing between the transcriptional regulatory sequences in the 5? leader region of the positive-strand viral genome and negative-strand 3? subgenomic region. In practicing the method, infected cells are exposed to the compound in an amount effective to inhibit viral replication.