Abstract: Provided herein are compositions, methods, and systems for targeted delivery of nucleic acids, including DNA and RNA, to a target cell. Also provided are compositions, methods, and systems for expressing a transgene in a cell by genomic editing. Further provided are compositions, methods, and systems for knocking in a gene-of-interest (GOI) into a target genomic locus in the genome, in particular the locus of albumin gene. Also provided are compositions, methods, and systems for treating a subject having or suspected of having a disorder or health condition employing ex vivo and/or in vivo genome editing.

Abstract: A working machine has a body and a load handling apparatus coupled to the body. The load handling apparatus is moveable with respect to the body by an electrically driven actuator assembly. A controller is configured to receive a tilt signal representative of a moment of tilt of the working machine and issue a control signal configured to control an electrical drive element of the electrically driven actuator assembly based on the value of the tilt signal relative to a tilt threshold.

Abstract: Provided include compositions, methods, and systems for modulating the expression, function, and/or activity of a target gene, for example a blood-clotting protein such as Factor VIII (FVIII), in a cell by genome editing. Also provided include compositions, methods, and systems for treating a subject having or suspected of having a disorder or health condition, e.g., Hemophilia A, employing ex vivo and/or in vivo genome editing.

Abstract: Provided include materials and methods for treating Hemophilia A in a subject ex vivo or in vivo. Also provided include materials and methods for knocking in a FVIII-encoding gene in a genome, in particular the locus of albumin gene.

Abstract: Provided include compositions, methods, and systems for modulating the expression, function, and/or activity of a target gene, for example a blood-clotting protein such as Factor VIII (FVIII), in a cell by genome editing. Also provided include compositions, methods, and systems for treating a subject having or suspected of having a disorder or health condition, e.g., hemophilia A, employing ex vivo and/or in vivo genome editing.

Abstract: A computer-implemented method includes determining, by a processor, an authenticity of a software object and its supply chain and providing an authenticity result indicative thereof; determining, by the processor, an integrity of the software object and its supply chain and providing an integrity result indicative thereof; and determining, by the processor, from the authenticity result and the integrity result, a score indicative of an amount of trust in the supply chain of the software object and in the software object, wherein the score is indicative of an amount of trust that the software object will work correctly if installed in a system that utilizes the software object.

Abstract: A working machine including a machine body having an operator cab, a ground engaging propulsion structure to permit movement of the machine over the ground, a load handling apparatus coupled to the machine body and moveable by a movement actuator with respect to the machine body, and an electric energy storage unit for providing power to the working machine. The working machine includes a longitudinal axis, wherein the operator cab is positioned towards a first side of the working machine with respect to the longitudinal axis, and the electric energy storage unit is positioned towards a second side of the working machine with respect to the longitudinal axis, wherein the first and second sides are located opposite each other.

Abstract: Provided herein, in some embodiments, are materials and methods for treating hemophilia A in a subject ex vivo or in vivo. Also provided herein, in some embodiments, are materials and methods for knocking in a coding sequence encoding a synthetic FVIII having a B domain substitute into a genome.

Abstract: A working machine has a body and a load handling apparatus coupled to the body. The load handling apparatus is moveable with respect to the body by an electrically driven actuator assembly. A controller is configured to receive a tilt signal representative of a moment of tilt of the working machine and issue a control signal configured to control an electrical drive element of the electrically driven actuator assembly based on the value of the tilt signal relative to a tilt threshold.

Abstract: The disclosed systems and methods improve on the current landscape surrounding autonomous “programmatic” advertising. The systems and methods incorporate an open source library that advertising publishers can deploy as an auction clearinghouse, setting a universal timeout and comparing all of the responses before sending the winning bids to the publisher's ad server. As an improvement, the present invention further stores the meta data associated with a winning bid on a specialized network for conducting asynchronous communications. This stored or “historical bid data” is then used to improve the speed, efficiency, profitability, and yield of online advertising. In addition, while traditional methodology for pre-bidding only benefits the publishers, the present invention suggests systems and methods wherein users and publishers may participate and be compensated for providing targeted bid data through either FIAT or cryptocurrency.

Abstract: Provided include materials and methods for treating Hemophilia A in a subject ex vivo or in vivo. Also provided include materials and methods for knocking in a FVIII-encoding gene in a genome, in particular the locus of albumin gene.

Abstract: A working machine including a ground engaging structure and a propulsion system for moving the working machine via the ground engaging structure. A body is supported on the ground engaging structure, and a working arm is connected to the body and has a carriage at one end for receiving an attachment. A control system is provided for selectively and variably oscillating the carriage.

Abstract: An additive manufacturing (AM) apparatus includes a build chamber, a build platform disposed within the build chamber for supporting an AM build part therein, and a build material deposition device. The apparatus further includes an energization arrangement having at least one energization source. The energization arrangement is capable of, in a fusion energy operation, selectively applying energy to a layer of build material to fuse the build material to form a next-to-be-produced layer of the AM build part and, in a material processing operation, selectively applying energy to and processing the surface of a last-produced layer of the AM build part, the layer of build material, and/or a surface of the next-to-be-produced layer.

Abstract: An aero-optical disturbance measurement system includes a mirror supported by a gimbal for receiving a light beam from a light emitting source, reflecting the light beam to a first periscope fold mirror and therefrom reflecting the light beam directly to a second periscope fold mirror. A first concave off-axis paraboloid mirror receives the light beam reflected from second periscope fold mirror and therefrom a first fold mirror receives the light beam reflected directly from first concave off-axis paraboloid mirror. A second fold mirror receives the light beam reflected directly from the first fold mirror. A second concave off-axis paraboloid mirror receives the light beam reflected directly from second fold mirror which reflects the light beam to a fast steering mirror. A fine tracker camera coupled to an embedded processer receives portion of light beam from fast steering mirror. Embedded processor controls movement of fast steering mirror and gimbal.

Abstract: A method of weighing a load lifted by a lifting arm of a machine is disclosed and includes receiving a signal from an axle sensor, said signal representing a force exerted on an axle as a result of lifting the load, receiving a further signal from a further sensor, said further signal representing an angle or slope of the lifting arm relative to a chassis of the machine, and calculating, from the received signal and the received further signal, the weight of the load lifted by the lifting arm.

Abstract: The aircraft includes a fuselage and at least one aerodynamic interface surface. It further includes a gimbaled mirror system which includes a mirror supported by a gimbal to receive a light beam from a light emitting source and reflect the light beam to a first periscope fold mirror. A wave front measuring system includes at least five reflective which reflects the light beam to a fast steering mirror. The fast steering mirror reflects the light beam directly to a beam splitter wherein the light beam is split into a transmitted portion and a reflected portion of the light beam.

Abstract: A working machine comprising a ground engaging structure and a propulsion system for moving the working machine via the ground engaging structure. A body is supported on the ground engaging structure, and a working arm is connected to the body and has a carriage at one end for receiving an attachment. A control system is provided for selectively and variably oscillating the carriage.