Abstract: Bacterial cultures are provided that comprise a modified Pseudomonas aeruginosa bacterium missing or deficient in two or more virulence factors. The two or more virulence factors can be selected from exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, alpha-1,3-rhamnosyltransferase, and 3-phosphoshikimate 1-carboxyvinyltransferase. Certain of the modified Pseudomonas aeruginosa bacteria are also missing or deficient in one or more alginate acetylation enzymes including the alginate O-acetyltransferases AlgI, AlgJ, AlgF, AlgX, and/or the C5-mannuronan epimerase AlgG. Methods of producing alginate are also provided along with compositions comprising alginate produced by the modified Pseudomonas aeruginosa bacteria.

Abstract: A data recorder is provided including a removable cartridge which carries both a sales form and an underlying log sheet. The fixed and variable data are imprinted by reciprocation of a platen carriage, with return of the carriage to the home position causing ejection of the credit card and loading of a stored energy mechanism. When the sales form is removed, the stored energy mechanism is released, whereby the log sheet is incrementally advanced to the next position.

Abstract: An improved mechanism for properly aligning a rotatable indicia carrying member and for locking said rotatable indicia carrying member in place. Both the aligning and locking mechanisms are formed as integral parts of a single frame unit and are operative to perform their respective aligning and locking functions during movement of the frame unit into engagement with the rotatable member.