Abstract: Aspects of the present disclosure include a graphical user interface for processing flow cytometer data, such as for group-wise analysis of the flow cytometer data. The graphical user interface according to certain embodiments includes a first pane configured to display one or more compound populations having events generated from flow cytometry data of one or more samples comprising particles irradiated by a light source in a flow stream, a second pane configured to display data gates applied to each of the compound populations and a third pane configured to display data files for each of the irradiated samples used to generate the compound populations. Systems having an input module for receiving flow cytometer data and processor with memory having instructions for displaying and implementing commands from the graphical user interface are described. Non-transitory computer readable storage medium and methods for using the graphical user interface are also provided.

Abstract: Aspects of the present disclosure include methods for processing cytometer data, such as for group-wise analysis of the cytometer data (e.g., flow cytometry data in FCS format, mass cytometry data, genomic cytometry data). Methods according to certain embodiments include generating a compound population of events that include data accessors from cytometry data, such as where the compound population of cytometer data is from two or more different samples retained as separate raw data files (e.g., are not concatenated to form a single combined data file). Systems having an input module for receiving cytometer data and processor with memory having instructions for practicing the subject methods are also described. Non-transitory computer readable storage medium is also provided.