Abstract: Provided herein are compositions, systems, and methods comprising effector proteins and uses thereof. These effector proteins are shown to be active with guide RNAs and may be characterized as CRISPR-associated (Cas) proteins. Various compositions, systems, and methods of the present disclosure leverage the activities of these effector proteins for the modification, detection, and engineering of nucleic acids.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: A virtual meeting system is discussed herein. An online meeting application can provide a parallel virtual meeting between or among two or more participants of a main virtual meeting. The parallel virtual meeting permits two or more participants of the main virtual meeting to have a “smaller-sized” meeting in parallel with the main virtual speaker or presentation while still participating in the main virtual meeting. Therefore, the participants of the parallel virtual meeting do not have to connect via another device or application and can avoid disrupting the main virtual meeting. The parallel virtual meeting can permit two separate meetings, such as one meeting with all participants and one meeting with a subset of all the participants.

Abstract: A virtual meeting system is discussed herein. An online meeting application places participants in their own virtual location with a common virtual meeting space and adjusts the audio volume and balance on a listener's device to simulate the virtual location of the participant who is speaking. The application includes a trigonometric function to adjust the audio volume and balance based on the location of the listener relative to the speaker. The application can also permit the participants to select their own virtual locations upon entry to the virtual meeting, can assign a virtual location or adjust audio based on a participant's settings, or can allow for parallel meetings.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: A virtual meeting system is discussed herein. An online meeting application places participants in their own virtual location with a common virtual meeting space and adjusts the audio volume and balance on a listener's device to simulate the virtual location of the participant who is speaking. The application includes a trigonometric function to adjust the audio volume and balance based on the location of the listener relative to the speaker. The application can also permit the participants to select their own virtual locations upon entry to the virtual meeting, can assign a virtual location or adjust audio based on a participant's settings, or can allow for parallel meetings.

Abstract: A validation record chain that is generated for a particular version of a software package may be used to verify the legitimacy of the particular version. A hash that is generated by a software building platform for a particular version of a software package is received. A validation record chain for the particular version is then generated that includes a plurality of certificates such that a first certificate in the validation record chain contains the hash, and each of one or more subsequent certificates is signed with a corresponding hash signature of a corresponding certifier application and contains a prior hash signature of a previous certificate in the validation record chain. The validation record chain is stored for validation of the particular version of the software package via the plurality of certificates.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: A surgical instrument for treating body tissues through narrow body passages employs an elongated cantilevered beam having a proximal end supported in a rigid block and a narrower distal end extendable through the narrow passages. One or more piezoelectric actuators are fixed to the beam surface and energized from an AC source provided by an electrical excitation system through electrodes interspersed with the piezoelectric actuators to produce oscillatory motion of the beam distal end in multiple modes of movement.

Abstract: A validation record chain that is generated for a particular version of a software package may be used to verify the legitimacy of the particular version. A hash that is generated by a software building platform for a particular version of a software package is received. A validation record chain for the particular version is then generated that includes a plurality of certificates such that a first certificate in the validation record chain contains the hash, and each of one or more subsequent certificates is signed with a corresponding hash signature of a corresponding certifier application and contains a prior hash signature of a previous certificate in the validation record chain. The validation record chain is stored for validation of the particular version of the software package via the plurality of certificates.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.

Abstract: An exemplary shingle includes at least one coated shingle sheet defining a headlap portion and a tab portion each having opposed upper and lower surfaces. A first line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a first thermally activated adhesive material. A second line of adhesive is adhered to one of the upper surface of the headlap portion and the lower surface of the tab portion, and includes a second thermally activated adhesive material having a minimum activation temperature less than a minimum activation temperature of the first thermally activated adhesive material.