Patents by Inventor Richard Haugland
Richard Haugland has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9513284Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.Type: GrantFiled: November 14, 2013Date of Patent: December 6, 2016Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Richard Haugland, Wai-Yee Leung, Jixiang Liu
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Patent number: 9382425Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.Type: GrantFiled: October 7, 2014Date of Patent: July 5, 2016Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Richard Haugland, Stephen Yue
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Publication number: 20160025713Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.Type: ApplicationFiled: October 6, 2015Publication date: January 28, 2016Inventors: Kyle Gee, Courtenay Hart-Kerndt, Wai-Yee Leung, Wayne Patton, Aleksey Rukavishnikov, Richard Haugland, Zhenjun Diwu
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Patent number: 9164099Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.Type: GrantFiled: May 8, 2013Date of Patent: October 20, 2015Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Kyle Gee, Aleksey Rukavishnikov, Courtenay Hart Kerndt, Richard Haugland, Wai-Yee Leung, Wayne Patton, Zhenjun Diwu
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Publication number: 20150031579Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.Type: ApplicationFiled: October 7, 2014Publication date: January 29, 2015Inventors: Richard HAUGLAND, Stephen Yue
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Publication number: 20140349893Abstract: The invention describes methods for labeling or detecting of immobilized poly(amino acids), including peptides, polypeptides and proteins, on membranes and other solid supports, using fluorescent dipyrrometheneboron difluoride dyes. Such immobilized poly(amino acids) are labeled or detected on blots or on arrays of poly(amino acids), or are attached to immobilized aptamers.Type: ApplicationFiled: April 25, 2014Publication date: November 27, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Richard HAUGLAND, Karen MARTIN, Wayne PATTON
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Patent number: 8883415Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.Type: GrantFiled: March 4, 2013Date of Patent: November 11, 2014Assignee: Life Technologies CorporationInventors: Richard Haugland, Stephen Yue
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Publication number: 20140193926Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.Type: ApplicationFiled: November 14, 2013Publication date: July 10, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Richard HAUGLAND, Wai-Yee Leung, Jixiang Liu
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Publication number: 20140038856Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.Type: ApplicationFiled: May 8, 2013Publication date: February 6, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kyle GEE, Aleksey RUKAVISHNIKOV, Courtenay HART, Richard HAUGLAND, Wai-Yee LEUNG, Wayne PATTON, Zhenjun DIWU
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Patent number: 8614294Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.Type: GrantFiled: August 23, 2012Date of Patent: December 24, 2013Assignee: Life Technologies CorporationInventors: Richard Haugland, Wai-Yee Leung, Jixiang Liu
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Patent number: 8535894Abstract: Provided are labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Discrete subsets of labeling reagent and immune-labeled complexes are provided that facilitate the simultaneous detection of multiple targets in a sample-complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.Type: GrantFiled: September 11, 2012Date of Patent: September 17, 2013Assignee: Life Technologies CorporationInventors: Robert Archer, Richard Haugland, Rosaria Haugland, Joseph Beechem, David Hagen
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Publication number: 20130079497Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.Type: ApplicationFiled: August 23, 2012Publication date: March 28, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Richard Haugland, Wai-Yee Leung, Jixiang Liu
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Patent number: 8304195Abstract: The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab? fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody.Type: GrantFiled: March 12, 2008Date of Patent: November 6, 2012Assignee: Life Technologies CorporationInventors: David Hagen, Joseph Beechem, Richard Haugland, Robert Archer, Rosaria Haugland
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Publication number: 20120270757Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.Type: ApplicationFiled: March 26, 2012Publication date: October 25, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kyle GEE, Courtenay Hart, Wai-Yee Leung, Wayne Patton, Aleksey Rukavishnikov, Richard Haugland, Zhenjun Diwu
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Patent number: 8268886Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.Type: GrantFiled: May 31, 2011Date of Patent: September 18, 2012Assignee: Life Technologies CorporationInventors: Richard Haugland, Wai-Yee Leung, Jixiang Liu
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Patent number: 8252530Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.Type: GrantFiled: January 4, 2011Date of Patent: August 28, 2012Assignee: Life Technologies CorporationInventors: Richard Haugland, Stephen Yue
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Publication number: 20120045848Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.Type: ApplicationFiled: May 31, 2011Publication date: February 23, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Richard Haugland, Wai-Yee Leung, Jixiang Liu
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Patent number: 8039642Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.Type: GrantFiled: October 12, 2007Date of Patent: October 18, 2011Assignee: Life Technologies CorporationInventors: Jixiang Liu, Richard Haugland, Wai-Yee Leung
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Publication number: 20110021372Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.Type: ApplicationFiled: June 22, 2010Publication date: January 27, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Brian AGNEW, Joseph Beechem, Kyle Gee, Richard Haugland, Thomas Steinberg, Wayne Patton
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Patent number: 7871773Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.Type: GrantFiled: June 1, 2007Date of Patent: January 18, 2011Assignee: Life Technologies CorporationInventors: Richard Haugland, Stephen Yue