Abstract: A cartridge with an expanding bullet that has advantageous terminal effects over an extended range. The expanding bullet including a bullet body including a metal jacket extending from a tail portion to a nose portion and surrounding an interior solid core and defining a forward opening and interior cavity. A tip has an exterior surface substantially flush with an exterior surface of the metal jacket. The tip has a main portion forward of the opening and a tip retention portion that at least partially fills the interior cavity. In certain embodiments the tip retention portion includes one or more fluid entry facilitation means such as a fracture regions configured to, upon impact of the bullet with a target, fracture or deform to expose one or more fluid pathways into the interior cavity and to a forward facing interior surface for initiating expansion of the expanding bullet.

Abstract: Devices and methods for a bullet with a strengthened mouth for improved penetration and expansion. In embodiments, a portion of the jacket is folded to provide double walls at the mouth. In embodiments, the jacket and core are partially bonded and partially separated.

Abstract: A cartridge comprising an elongate rifle bullet with a plurality of circumferential grooves having overmolded polymer therein defining embedded polymer rings. The cartridge further having a case and propellant. The polymer rings have an outer surface that is flush with, that is, conforming to the outer surface of the body. The polymer may have be selected to have a favorable coefficient of friction with respect to the barrel. The bands offering reduction of the metal to metal contact between the bullet and the barrel while not diminishing the ballistic coefficient of the bullet.

Abstract: A cartridge with an expanding bullet that has advantageous terminal effects over an extended range. The expanding bullet including a bullet body including a metal jacket extending from a tail portion to a nose portion and surrounding an interior solid core and defining a forward opening and interior cavity. A tip has an exterior surface substantially flush with an exterior surface of the metal jacket. The tip has a main portion forward of the opening and a tip retention portion that at least partially fills the interior cavity. In certain embodiments the tip retention portion includes one or more fluid entry facilitation means such as a fracture regions configured to, upon impact of the bullet with a target, fracture or deform to expose one or more fluid pathways into the interior cavity and to a forward facing interior surface for initiating expansion of the expanding bullet.

Abstract: A cartridge comprising an elongate rifle bullet with a plurality of circumferential grooves having overmolded polymer therein defining embedded polymer rings. The cartridge further having a case and propellant. The polymer rings have an outer surface that is flush with, that is, conforming to the outer surface of the body. The polymer may have be selected to have a favorable coefficient of friction with respect to the barrel. The bands offering reduction of the metal to metal contact between the bullet and the barrel while not diminishing the ballistic coefficient of the bullet.

Abstract: A cartridge comprising an elongate rifle bullet with a plurality of circumferential grooves having overmolded polymer therein defining embedded polymer rings. The cartridge further having a case and propellant. The polymer rings have an outer surface that is flush with, that is, conforming to the outer surface of the body. The polymer may have be selected to have a favorable coefficient of friction with respect to the barrel. The bands offering reduction of the metal to metal contact between the bullet and the barrel while not diminishing the ballistic coefficient of the bullet.

Abstract: A cartridge with an expanding bullet that has advantageous terminal effects over an extended range. The expanding bullet including a bullet body including a metal jacket extending from a tail portion to a nose portion and surrounding an interior solid core and defining a forward opening and interior cavity. A tip has an exterior surface substantially flush with an exterior surface of the metal jacket. The tip has a main portion forward of the opening and a tip retention portion that at least partially fills the interior cavity. In certain embodiments the tip retention portion includes one or more fluid entry facilitation means such as a fracture regions configured to, upon impact of the bullet with a target, fracture or deform to expose one or more fluid pathways into the interior cavity and to a forward facing interior surface for initiating expansion of the expanding bullet.

Abstract: A cartridge with an expanding bullet that has advantageous terminal effects over an extended range. The expanding bullet including a bullet body including a metal jacket extending from a tail portion to a nose portion and surrounding an interior solid core and defining a forward opening and interior cavity. A tip has an exterior surface substantially flush with an exterior surface of the metal jacket. The tip has a main portion forward of the opening and a tip retention portion that at least partially fills the interior cavity. In certain embodiments the tip retention portion includes one or more fluid entry facilitation means such as a fracture regions configured to, upon impact of the bullet with a target, fracture or deform to expose one or more fluid pathways into the interior cavity and to a forward facing interior surface for initiating expansion of the expanding bullet.

Abstract: A cartridge comprising an elongate rifle bullet with a plurality of circumferential grooves having overmolded polymer therein defining embedded polymer rings. The cartridge further having a case and propellant. The polymer rings have an outer surface that is flush with, that is, conforming to the outer surface of the body. The polymer may have be selected to have a favorable coefficient of friction with respect to the barrel. The bands offering reduction of the metal to metal contact between the bullet and the barrel while not diminishing the ballistic coefficient of the bullet.

Abstract: A cartridge comprising an elongate rifle bullet with a plurality of circumferential grooves having overmolded polymer therein defining embedded polymer rings. The cartridge further having a case and propellant. The polymer rings have an outer surface that is flush with, that is, conforming to the outer surface of the body. The polymer may have be selected to have a favorable coefficient of friction with respect to the barrel. The bands offering reduction of the metal to metal contact between the bullet and the barrel while not diminishing the ballistic coefficient of the bullet.

Abstract: A cartridge with an expanding bullet that has advantageous terminal effects over an extended range. The expanding bullet including a bullet body including a metal jacket extending from a tail portion to a nose portion and surrounding an interior solid core and defining a forward opening and interior cavity. A tip has an exterior surface substantially flush with an exterior surface of the metal jacket. The tip has a main portion forward of the opening and a tip retention portion that at least partially fills the interior cavity. In certain embodiments the tip retention portion includes one or more fluid entry facilitation means such as a fracture regions configured to, upon impact of the bullet with a target, fracture or deform to expose one or more fluid pathways into the interior cavity and to a forward facing interior surface for initiating expansion of the expanding bullet.

Abstract: A novel limited primer extension reaction improves detection sensitivity and specificity in a variety of hybridization platforms. In the invention, a sequence of target DNA that lacks one of the four types of nucleic acid bases for a span of eight or more adjacent nucleotide positions is selected for use. This sequence is referred to as the extension complement sequence, or ECS. A primer with a sequence that is complementary to the target sequence that is immediately downstream (to the 3? side) of this ECS is used to initiate an extension reaction. Extension occurs using a DNA polymerase and standard deoxynucleoside triphosphates for three of the four types of nucleic acid bases. The fourth base, which is complementary to the base missing in the ECS, is either absent or present only in the form of a dideoxynucleoside triphosphate, which does not support further extension. In either case, the extension reaction does not proceed past the first occurrence in the template of the base that is missing in the ECS.

Abstract: The present invention is directed to a method of extracting nucleic acids from samples containing prokaryotic cells and/or eukaryotic cells by (a) lysing the cells under conditions that minimize RNA expression or enzymatic degradation of RNA; (b) dispersing and diluting the lysed cells; (c) mixing the dispersed and diluted lysed cells with a buffered solution containing cationic and anionic detergents; (d) separating non-nucleic acid contaminants from the nucleic acids by mixing with an organic extraction solvent to generate an emulsion that separates into an organic phase, an interface containing the contaminants and an aqueous phase containing the nucleic acids; (e) separating the phases, and retaining the aqueous phase; and (f) precipitating the nucleic acids in the aqueous phase.

Abstract: A novel limited primer extension reaction improves detection sensitivity and specificity in a variety of hybridization platforms. In the invention, a sequence of target DNA that lacks one of the four types of nucleic acid bases for a span of eight or more adjacent nucleotide positions is selected for use. This sequence is referred to as the extension complement sequence, or ECS. A primer with a sequence that is complementary to the target sequence that is immediately downstream (to the 3? side) of this ECS is used to initiate an extension reaction. Extension occurs using a DNA polymerase and standard deoxynucleoside triphosphates for three of the four types of nucleic acid bases. The fourth base, which is complementary to the base missing in the ECS, is either absent or present only in the form of a dideoxynucleoside triphosphate, which does not support further extension. In either case, the extension reaction does not proceed past the first occurrence in the template of the base that is missing in the ECS.

Abstract: A novel polymeric nucleic acid probe improves detection sensitivity and specificity in a variety of hybridization platforms. The probe is made up of multiple short nucleic acid sequences (referred to as monomers) attached together to form a long polymeric probe for use in hybridization applications. For applications requiring immobilization of the probes to a surface, the polymeric probes are similar to long DNA probes in that they can be immobilized to a variety of surfaces without need for a chemical modification to the end of the probe. Because target nucleic acids hybridize to the relatively short monomers in the polymeric probe, the polymeric probes are more specific than long DNA probes. In addition, polymeric probes also improve the signal-to-background ratio by increasing the number of accessible monomer oligonucleotide probes immobilized per unit area on a surface.