Abstract: The specification provides methods for introducing a desired genetic change in a nucleotide sequence using a double-strand break (DSB)-inducing genome editing system, the method comprising: identifying one or more available cut sites in a nucleotide sequence; analyzing the nucleotide sequence and available cut sites with a computational model to identify the optimal cut site for introducing the desired genetic change into the nucleotide sequence; and contacting the nucleotide sequence with a DSB-inducing genome editing system, thereby introducing the desired genetic change in the nucleotide sequence at the cut site.

Abstract: Provided herein is an improved method for performing CRISPR/Cas based screening that is not dependent on viral cloning methods.

Abstract: A dispenser of sanitary finger covers which are sanitized individually at the time of use. The system uses either a battery operated ultraviolet LED or spray disinfectant to automatically or manually sanitize the finger covers. The sanitary finger covers can be rapidly applied without contamination using a single finger. Removal and disposal are similarly rapid and easy. The finger covers are inexpensive and may be made from a variety of readily available and biodegradable materials. The dispenser is lightweight and portable. It can be carried by the user or attached to their uniform. The dispenser can be easily replenished with finger covers once empty, using a provided cartridge prefilled with finger covers.

Abstract: A dispenser of sanitary finger covers which are sanitized individually at the time of use. The system uses either a battery operated ultraviolet LED or spray disinfectant to automatically or manually sanitize the finger covers. The sanitary finger covers can be rapidly applied without contamination using a single finger. Removal and disposal are similarly rapid and easy. The finger covers are inexpensive and may be made from a variety of readily available and biodegradable materials. The dispenser is lightweight and portable. It can be carried by the user or attached to their uniform. The dispenser can be easily replenished with finger covers once empty, using a provided cartridge prefilled with finger covers.

Abstract: A door handle assembly is provided that includes a cover element and a grip element. The cover element is movable between a flush position where the cover element fills an opening and is flush with a visible face of an outer door panel, and a retracted position where the cover element is displaced inwardly from the opening and the grip element and outer door panel defines a finger access space to receive fingers of a user. The assembly further includes an actuator to move the cover between the flush and retracted position, a hand sensor attached to the grip element to detect the user's fingers, a detection device to cooperate with a complementary transmitting device to identify the user, a latching mechanism unlatch the vehicle door when fingers are detected by the hand sensor, and a control unit to control the hand sensor, detection device, latching mechanism, and actuator.

Abstract: A door handle assembly for a vehicle door is provided that includes: a housing assembly fixedly connected to an outer door panel of the vehicle door having a visible face defining an opening, a cover assembly having a cover and a grip element, the cover assembly is movable between a flush position where the cover element fills the opening and is flush with the visible face and a fully retracted position where the cover element is displaced inwardly from the opening, and the grip element and housing assembly defines an access space to receive fingers of a user, a displacement mechanism to move the cover assembly, and a pin connected to the housing assembly that interacts with a cam profile formed on an external surface of the cover assembly such that the cover assembly follows a path during movement from the flush to fully retracted position.

Abstract: Provided herein is an improved method for performing CRISPR/Cas based screening that is not dependent on viral cloning methods.

Abstract: A method for biometric identification for use with a computing device is provided herein. The method includes capturing a temporal sequence of images of the face of a user at different locations within a three-dimensional interaction space. The method further includes extracting one or more face descriptors from the images and generating a biometric template compiling the face descriptors.

Abstract: A method for biometric identification for use with a computing device is provided herein. The method includes capturing a temporal sequence of images of the face of a user at different locations within a three-dimensional interaction space. The method further includes extracting one or more face descriptors from the images and generating a biometric template compiling the face descriptors.

Abstract: A method for biometric identification for use with a computing device is provided herein. The method includes capturing a temporal sequence of images of the face of a user at different locations within a three-dimensional interaction space. The method further includes extracting one or more face descriptors from the images and generating a biometric template compiling the face descriptors.

Abstract: Methods and apparatus for processing biometric digit data variously include scanning a digit in a transverse direction relative to an array of sensor elements, detecting the scanned digit and/or a predetermined characteristic of the scanned digit, outputting from individual sensor elements of the array of sensor elements respective continuous streams of biometric data associated with the scanned digit, discrete sampling the respective continuous streams of data, and reconstructing the discrete sampled data. The discrete sampled data may be directly reconstructed, e.g., in accordance with a reconstruction characteristic associated with detecting and/or discrete sampling the scanned digit, to form a biometric characteristic image associated with the digit. Optionally, the respective continuous streams of data may be stored for later discrete sampling, or discrete sampled data may be stored for later reconstruction to form a biometric characteristic image associated with the digit.

Abstract: Provided is a fingerprint sensor configured for detecting a pulse of a human or other mammal. A time series of fingerprint images is analyzed to identify time-based variations in spatial location and/or pressure from ridges of a finger, where the variations result from time-based variations in the flow of blood. Based on the analysis, a determination is made if the time-based variations are indicative of a pulse in a digit of the person, and if so, a determination is made as to the pulse rate based on the variations. Imaging sensitivity of the digit at the fingerprint sensor is optimized for determination of the pulse. The time series of fingerprint images is also analyzed to determine if there is bulk motion of the digit, in which case pulse rate analysis is temporarily suspended.

Abstract: Provided is a method for analyzing image slices. The method includes extracting first and second sub-slices from first and second image slices, respectively, and computing a shift between the first and second image slices based on the first and second sub-slices. The first and second sub-slices overlap. Also provided is a method for controlling a cursor on a screen. The method includes determining a shift between a first image slice and a second image slice and determining a displacement of the cursor on the screen based on the determined shift.

Abstract: Provided herein is a method of making an integrated circuit device using copper metallization on 1-3 PZT composite. The method includes providing an overlay of electroplated immersion of gold (Au) to cover copper metal traces, the overlay preventing oxidation on 1:3 PZT composite with material. Also included is the formation of immersion Au nickel electrodes on the 1-3 PZT composite to achieve pad metallization for external connections.

Abstract: A method for biometric identification for use with a computing device is provided herein. The method includes capturing a temporal sequence of images of the face of a user at different locations within a three-dimensional interaction space. The method further includes extracting one or more face descriptors from the images and generating a biometric template compiling the face descriptors.

Abstract: Invention includes a novel pointing device which uses acoustic impediography as a means to locate the position of finger and then uses the said location to control the position of a cursor on a computer screen. In addition, while the finger is touching the sensor the touch-pressure level can be estimated via statistical data evaluation, as average brightness decreases with increasing touch-pressure providing a means for gesturing. This new device has the advantage that it can double as a biometric identification device for verifying the identity of the computer's user.

Abstract: Provided is a fingerprint sensor configured for detecting a pulse of a human or other mammal. A time series of fingerprint images is analyzed to identify time-based variations in spatial location and/or pressure from ridges of a finger, where the variations result from time-based variations in the flow of blood. Based on the analysis, a determination is made if the time-based variations are indicative of a pulse in a digit of the person, and if so, a determination is made as to the pulse rate based on the variations. Imaging sensitivity of the digit at the fingerprint sensor is optimized for determination of the pulse. The time series of fingerprint images is also analyzed to determine if there is bulk motion of the digit, in which case pulse rate analysis is temporarily suspended.

Abstract: Runtime services for network software platforms are described. In one implementation, runtime services are configured for a web application. A configuration file is created containing tags that provide references to configuration information specific to operational behavior of the web application. The configuration file is read upon receiving a request to start the web application. The runtime services for the web application are initialized based on the configuration information read from the configuration file.

Abstract: Populations enriched for myogenic progenitors are obtained by selection on the basis of expression of specific cell surface markers. The muscle progenitor cells are characterized as being CD45? and CD34+, and may further be characterized as lacking expression of Mac-1 (CD11b) and positive for expression of CXCR4 (CD184) and ?1-integrin (CD29).

Abstract: Synchronized video playback among multiple users across a network provides a fully social experience where people in different locations may be enabled to watch the same video in a “virtual living room.” The users may be represented graphically, as avatars, in front of the video, and may be enabled to use animations, text chat, and voice chat to interact with each other. Thus, a group of people may be enabled to share the experience of watching a video together as if they were in the same room, without being physically present together.