Abstract: The present disclosure relates to nonstructural protein 1 (NS1) from flaviviruses and uses thereof. In particular, the present invention relates to diagnostic and therapeutic uses of NS1 to treat and prevent disease caused by flaviviruses.

Abstract: In one embodiment, 3-Aminopropyltriethoxysilane (APTES) functionalized graphene oxide (APTES-GO) wrapped SiO2 particle composite (SiO2@APTES-GO) was prepared via the self-assembly process of APTES-GO sheets and SiO2 particles. Transmission electron microscopy (TEM) and Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR) confirmed wrapping of the SiO2 particles by the APTES-GO sheets. A biosensor based on electrochemical impedance spectroscopy (EIS) was constructed and used to sensitively detect dengue DNA and dengue RNA via primer hybridization using different oligonucleotide sequences. The results demonstrated that the SiO2@APTES-GO electrode material led to enhanced sensitivity, selectivity and detection limit, compared to both APTES-GO and APTES-SiO2.

Abstract: The present disclosure relates to nonstructural protein 1 (NS1) from flaviviruses and uses thereof. In particular, the present invention relates to diagnostic and therapeutic uses of NS1 to treat and prevent disease caused by flaviviruses.

Abstract: The invention generally relates to compositions and methods for identifying dengue virus. In certain embodiments, the invention provides methods for identifying a dengue virus serotype that involve obtaining a sample comprising at least one dengue virus protein; digesting the protein to produce peptides; ionizing the peptides; detecting a parent and associated fragment ion of the peptides; and identifying the serotype based on results of the detecting step.

Abstract: Cells that produce inventive pseudotyped retroviruses having a broad host range have been produced. In one aspect of the invention, the cells produce retroviruses pseudotyped with at least two different viral glycoproteins, such as togaviral glycoproteins. In alternative embodiments, the cells produce retroviruses pseudotyped with filoviral glycoproteins. Methods of producing the above-described cells, as well as the pseudotyped retroviruses thus produced, are also provided. In other embodiments, methods of screening agents effective in blocking viral entry into a cell, including filoviral entry or entry of viruses having at least two different viral glycoproteins disposed in their lipid bilayer, such as togaviruses, are provided. Moreover, methods of using the inventive pseudotyped retroviruses for introducing nucleotide sequences into target cells, and kits for forming the inventive pseudotyped retroviruses, are also provided.

Abstract: A method and system for temporarily halting an article for sensing with a fixed sensing system without altering its average speed. While halted, the article's orientation is manipulated for seeking and presenting a specific area thereon for sensing. The time available for manipulation results from accelerating each article relative to the average speed of articles prior to sensing. The sensing of the article includes visually reading a label thereon to identify the article, and inspection of the article for determining its physical condition. If desired, the physical condition of the article such as a glass bottle may also be evaluated without halting movement thereof. Once the article has been identified and inspected, the article is categorized based on the visual information obtained. Articles in certain categories are rejected from those evaluated.

Abstract: A system for analyzing the physical condition of a moving article, such as a glass bottle, in a stream of articles of manufacture which are moving at a predetermined speed. A light source illuminates a portion of the moving article to be analyzed at an illumination angle, and a camera obtains an image of the illuminated portion of the article. The camera is disposed to view the article at a viewing angle that is distinct from the illumination angle, thereby substantially reducing the amount of reflected light received by the camera. A transparent surface is interposed between the camera and the article and angled to reduce the amount of reflected light received by the camera. A computer or the like processes the image to evaluate the physical condition of the article, and categorizes the article into distinct categories based upon the physical condition of the article.

Abstract: A media feed roll apparatus is provided for the transfer of articles such as envelopes, magazines and newspapers, one by one, from a stack to a transport which conveys these articles to a work station for printing or labeling. The apparatus and methodology according to the invention allow for independent adjustment of the roller nip between upper drive rollers and lower facing rollers so that an article such as an envelope can be inserted into the nip with the roller pairs providing substantially equal nip pressure on the article. Roller nip pressure is achieved through a mechanism allowing raising and lowering of the upper drive rollers, preferably by a pivotal action, which are independently suspended. Upon being released from the mechanism, the rollers are biased toward the article thereby creating substantially equal nip pressure on the article even if the nip spacing is different as a result of the article having different thicknesses.

Abstract: Apparatus for applying a piece of adhesive strip to a stack of sheets having a thickness within one of several thickness ranges, the width of the adhesive strip to be applied being dependent on the thickness range to which the thickness of the stack of sheets corresponds. The apparatus includes a pair of strip guides and means, responsive to the thickness of the stack of sheets, for moving the strip guides to initial positions, said means including linkage means. The apparatus also includes: bipartite clamping means; and means, coupled to the linkage means, for moving the clamping means into engagement with the stack of sheets and for stressing play out of said linkage means, thereby moving the strip guides into one of a set of several sets of positions, each set corresponding to one of the thickness ranges. Each set of positions is suitable for supporting a different one of a plurality of adhesive strips, each strip having a different but standard size width.