Abstract: An emblem assembly is provided that includes a power source, a backing member, and a light-producing assembly coupled to the power source and supported by the backing member. The light-producing assembly may include an electroluminescent light source. The emblem assembly further includes a chromatic layer over the light-producing assembly that comprises and emblem shape, and a translucent sealing structure over the chromatic layer that seals the backing member, the light-producing assembly, and the chromatic layer. The emblem assembly exhibits a chrome- or mirror-like finish when viewed under ambient lighting conditions. Further, the emblem assembly possesses a glowing appearance when activated under low light or nighttime conditions.

Abstract: An illuminated emblem assembly is provided that includes a power source, a backing member, and a light-producing assembly coupled to the power source and supported by the backing member. The light-producing assembly includes a plurality of light-emitting diode sources. The light-producing assembly may also include a plurality of micro-sized, dispersed light-emitting diode sources. The illuminated emblem assembly further includes a translucent base region over the light sources, a chromatic layer over the translucent base region and a UV-stable translucent sealing structure over the chromatic layer that seals the backing member, the light producing assembly, and the chromatic layer. The sealing structure may be UV-stable and/or include a design feature. The illuminated emblem assembly exhibits a chrome- or mirror-like finish when viewed under ambient lighting conditions. Further, the illuminated emblem assembly possesses a glowing appearance when activated under low light or nighttime conditions.

Abstract: 5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.

Abstract: Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N-terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications.

Abstract: Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.

Abstract: 5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.

Abstract: 5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.

Abstract: Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.

Abstract: 5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.

Abstract: Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N-terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications.

Abstract: An emblem assembly is provided that includes a power source, a backing member, and a light-producing assembly coupled to the power source and supported by the backing member. The light-producing assembly may include an electroluminescent light source. The emblem assembly further includes a chromatic layer over the light-producing assembly that comprises and emblem shape, and a translucent sealing structure over the chromatic layer that seals the backing member, the light-producing assembly, and the chromatic layer. The emblem assembly exhibits a chrome- or mirror-like finish when viewed under ambient lighting conditions. Further, the emblem assembly possesses a glowing appearance when activated under low light or nighttime conditions.

Abstract: An illuminated emblem assembly is provided that includes a power source, a backing member, and a light-producing assembly coupled to the power source and supported by the backing member. The light-producing assembly includes a plurality of light-emitting diode sources. The light-producing assembly may also include a plurality of micro-sized, dispersed light-emitting diode sources. The illuminated emblem assembly further includes a translucent base region over the light sources, a chromatic layer over the translucent base region and a UV-stable translucent sealing structure over the chromatic layer that seals the backing member, the light producing assembly, and the chromatic layer. The sealing structure may be UV-stable and/or include a design feature. The illuminated emblem assembly exhibits a chrome- or mirror-like finish when viewed under ambient lighting conditions. Further, the illuminated emblem assembly possesses a glowing appearance when activated under low light or nighttime conditions.

Abstract: Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N-terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications.

Abstract: Compositions and methods are provided for selection and enrichment of a target gene from a library of polynucleotide sequences such as might be formed from a genome or by random mutagenesis of a genetic sequence. The selection and enrichment occurs in aqueous droplets formed in an emulsion that compartmentalize individual polynucleotides from the library or a plurality of polynucleotides that may include polynucleotides not derived from the library, transcription and translation reagents and optionally additional chemical and enzyme reagents. The selection and enrichment method utilizes a polynucleotide adaptor which when ligated to the polynucleotide fragment enables amplification to occur in the presence of an adaptor specific primer.

Abstract: An illuminated vehicle emblem assembly is provided that includes a power source, a backing member, and a light-producing assembly coupled to the power source and supported by the backing member. The light-producing assembly may include an electroluminescent light source. The illuminated vehicle emblem assembly further includes a translucent base region over the light source, a chromatic layer over the translucent base region, and a translucent sealing structure configured to seal the backing member, the light producing assembly, and the chromatic layer. The illuminated vehicle emblem assembly exhibits a chrome- or mirror-like finish when viewed under ambient lighting conditions. Further, the illuminated vehicle emblem assembly possesses a glowing appearance when activated under low-light or night-time conditions.

Abstract: 5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.

Abstract: Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N-terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications.

Abstract: Specified restriction endonucleases have been characterized for the first time by their amino acid and DNA sequences. These sequences and those with at least 90% identity thereto have been used as probes in sequence similarity analyses to identify sequence matches in a sequence database that corresponds to novel restriction endonucleases or isoschizomers. The sequence similarity analyses includes selecting a positive sequence match from any sequence producing an expectation value of less than or equal to e?02.

Abstract: Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N-terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications.

Abstract: An illuminated vehicle emblem assembly is provided that includes a power source, a backing member, and a light-producing assembly coupled to the power source and supported by the backing member. The light-producing assembly may include an electroluminescent light source. The illuminated vehicle emblem assembly further includes a translucent base region over the light source, a chromatic layer over the translucent base region, and a translucent sealing structure configured to seal the backing member, the light producing assembly, and the chromatic layer. The illuminated vehicle emblem assembly exhibits a chrome- or mirror-like finish when viewed under ambient lighting conditions. Further, the illuminated vehicle emblem assembly possesses a glowing appearance when activated under low-light or night-time conditions.