Abstract: Halogen-free, microporous polyolefin membranes are disclosed herein. The halogen-free, microporous polyolefin membranes can be manufactured using an environmentally friendly manufacturing process that includes extrusion of polymer-plasticizer mixtures followed by sheet formation and extraction of the plasticizer with a halogen-free solvent. The halogen-free solvent has a flashpoint greater than about 23° C. and an initial boiling point at least about 50° C. lower than the flashpoint of the plasticizer. The process can further be a closed loop process in which the halogen-free solvent can be reused.

Abstract: In some implementations, a computing device can proactively determine a destination and request traffic information for routes from a starting location to the destination. In some implementations, a computing device can identify some routes between a starting location and a destination as non-recommended routes and recommend other routes. In some implementations, a computing device can rank routes between a starting location and a destination based on automatically-determined user interest. In some implementations, a computing device can determine a user is familiar with a route and adjust the information presented to the user about the route accordingly.

Abstract: Embodiments herein provide apparatuses and techniques for providing a notification indicating guidance to reaching cellular connectivity in the event of a loss of cellular connectivity and for guiding a user of a user equipment to a location with cellular connectivity. The notification may be generated based on expiration of a timer, a screen unlock operation, or an application connection failure. The notification may indicate how long ago the cellular connectivity loss occurred and how far away a nearest location is with a cellular connectivity was. The user equipment may display a map interface based on user input selecting the notification. The map interface may show a map of the current location of the user equipment, a nearest location with cellular connectivity to the user equipment, and a path, which includes a tracked path of user equipment and a proposed path to the nearest location with cellular connectivity.

Abstract: Embodiments herein provide apparatuses and techniques for providing a notification indicating guidance to reaching cellular connectivity in the event of a loss of cellular connectivity and for guiding a user of a user equipment to a location with cellular connectivity. The notification may be generated based on expiration of a timer, a screen unlock operation, or an application connection failure. The notification may indicate how long ago the cellular connectivity loss occurred and how far away a nearest location is with a cellular connectivity was. The user equipment may display a map interface based on user input selecting the notification. The map interface may show a map of the current location of the user equipment, a nearest location with cellular connectivity to the user equipment, and a path, which includes a tracked path of user equipment and a proposed path to the nearest location with cellular connectivity.

Abstract: Novel polypeptide compositions and methods for their use are provided comprising fusion proteins in which the polysaccharide binding domain or functional portion thereof of a polysaccharidase is fused to a heterologous protein or is conjugated to a chemical moiety. The compositions can be synthesized or prepared by recombinant DNA technology. The compositions find use as removable labels.

Abstract: Fusion proteins or conjugates are provided containing an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that binds to a .beta.-1,4-glycan matrix such as cellulose. The substrate binding region is essentially without polysaccharidase activity. In the fusion protein, the substrate binding region is fused or chemically linked to a polypeptide such as an enzyme, a hormone, an immunoglobulin or a protein dye. By contacting the fusion protein with a .beta.-1,4-glycan matrix, the substrate binding region binds to the matrix to immobilize the polypeptide on the matrix. The polypeptide or fusion protein can be removed from the matrix with a protease acting on a protease recognition sequence or with a solution having a low ionic strength or high pH. In the conjugate, the substrate binding region is joined such as by covalent bonding to a non-protein chemical moiety such as a dye, chromophore, fluorescor, radionuclide or enzyme co-factor.

Abstract: A cell feeder/harvester assembly including a primary platform with at least one recessed culture vessel holding area whereon two pipet tip racks, one for holding sterile pipet tips and the other rack for collecting used pipet tips, and an array of culture vessels are supported. An X assembly extends transversely across the primary platform and is driven longitudinally thereover, the X assembly having a Y assembly, including a secondary platform, disposed thereon which is adapted to be driven transversely over the X assembly, wherein both the X assembly and the Y assembly move so as to position a Z assembly, which is attached to a top of the secondary platform, over a select pair of culture vessels on opposite sides of the secondary platform. The Z assembly is adapted to service all culture vessels on the primary platform, without the need for manual or individualized directing, by removing waste products and adding necessary chemicals to the individual culture vessels.

Abstract: A fusion protein is prepared containing a polypeptide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with an affinity matrix containing a substrate such as cellulose for the cellulase substrate binding region, the substrate binding region binds to the affinity matrix to immobilize the polypeptide. The polypeptide can be purified by separating the fusion protein or polypeptide from the affinity matrix. The polypeptide can be separated by cleaving the protein with a Cellulomonas fimi protease.

Abstract: A fusion protein is prepared containing a polypeptide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with an affinity matrix containing a substrate such as cellulose for the cellulase substrate binding region, the substrate binding region binds to the affinity matrix to immobilize the polypeptide. The polypeptide can be purified by separating the fusion protein or polypeptide from the affinity matrix.

Abstract: Apparatus for severing continuous lengths of hollow fibers into predetermined, equal lengths for use as chemical vapor dispensers is provided.

Abstract: A brake rotor with a ceramic-metallic coating. The ceramic-metallic coating provides a wear resistant surface. The coating generally comprises about 30 to 70% by weight of ceramic particles in about 30 to 70% by weight metallic carrier material compatible with the substrate and carbide.