Abstract: Chimeric flaviviruses that are avirulent and immunogenic are provided. The chimeric viruses are constructed to contain amino acid mutations in the nonstructural proteins of a flavivirus. Chimeric viruses containing the attenuation-mutated nonstructural genes of the virus are used as a backbone into which the structural protein genes of a second flavivirus strain are inserted. These chimeric viruses elicit pronounced immunogenicity yet lack the accompanying clinical symptoms of viral disease. The attenuated chimeric viruses are effective as immunogens or vaccines and may be combined in a pharmaceutical composition to confer simultaneous immunity against several strains of pathogenic flaviviruses.

Abstract: Computer-implemented method and system for optimizing a finite element mesh. Computing devices are configured to calculate a quality score for at least one original element in a finite element mesh based upon two or more quality attributes simultaneously. A quality score for at least one substitute element for the original element is calculated based upon the two or more quality attributes. If one or more substitute element(s) have a quality score that is better than the quality score of the original element(s), the original element(s) is/are replaced with the substitute element(s) having the best quality score. A quality score may be calculated for each of a plurality of combinations of one or more substitute elements. Input may be provided specifying at least one quality threshold value for comparing the two or more element quality parameters (skew, warp, aspect ratio, etc.).

Abstract: Computer-implemented method and system for optimizing a finite element mesh. Computing devices are configured to calculate a quality score for at least one original element in a finite element mesh based upon two or more quality attributes simultaneously. A quality score for at least one substitute element for the original element is calculated based upon the two or more quality attributes. If one or more substitute element(s) have a quality score that is better than the quality score of the original element(s), the original element(s) is/are replaced with the substitute element(s) having the best quality score. A quality score may be calculated for each of a plurality of combinations of one or more substitute elements. Input may be provided specifying at least one quality threshold value for comparing the two or more element quality parameters (skew, warp, aspect ratio, etc.).