Abstract: A media recording element having capabilities to facilitate recording media for subsequent playback. The recording element further including features associated with limited operations of a hard drive of other similarly mechanical memory under certain operating conditions so as to limit power consumption and/or memory degradation.

Abstract: A media recording element having capabilities to facilitate recording media for subsequent playback. The recording element further including features associated with limited operations of a hard drive of other similarly mechanical memory under certain operating conditions so as to limit power consumption and/or memory degradation.

Abstract: A media recording element having capabilities to facilitate recording media for subsequent playback. The recording element further including features associated with limited operations of a hard drive of other similarly mechanical memory under certain operating conditions so as to limit power consumption and/or memory degradation.

Abstract: A media recording element having capabilities to facilitate recording media for subsequent playback. The recording element further including features associated with limited operations of a hard drive of other similarly mechanical memory under certain operating conditions so as to limit power consumption and/or memory degradation.

Abstract: A media recording element having capabilities to facilitate recording media for subsequent playback. The recording element further including features associated with limited operations of a hard drive of other similarly mechanical memory under certain operating conditions so as to limit power consumption and/or memory degradation.

Abstract: A computer system for processing a prescription for a prescription product prescribed by a healthcare provider (HCP) to a patient includes a memory device and a processor. The processor is programmed to receive patient data and insurance data from an HCP computing device, the patient data including a completed prescription form for the prescription product, and the insurance data identifying an insurance provider, wherein the prescription product is an antiviral product, store the patient data and the insurance data, determine a current electronic prior authorization request form, transmit the determined current electronic prior authorization request form to the HCP computing device, and prompt an HCP user to complete the determined current electronic prior authorization request form by enabling the HCP user to automatically populate at least one data field included within the determined current electronic prior authorization request form.

Abstract: A media recording element having capabilities to facilitate recording media for subsequent playback. The recording element further including features associated with limited operations of a hard drive of other similarly mechanical memory under certain operating conditions so as to limit power consumption and/or memory degradation.

Abstract: A media recording element having capabilities to facilitate recording media for subsequent playback. The recording element further including features associated with limiting operations of a hard drive or other similarly mechanical memory under certain operating conditions so as to limit power consumption and/or memory degradation.

Abstract: The present invention is directed to expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein ??? complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on the cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric G? protein is expressed in conjunction with nucleotide sequences from the yeast G protein ?? subunits. A second aspect of the present invention provides expression vectors and yeast cells transformed therewith encoding chimeric yeast/heterologous G protein coupled receptors.

Abstract: A media recording element having capabilities to facilitate recording media for subsequent playback. The recording element further including features associated with limiting operations of a hard drive or other similarly mechanical memory under certain operating conditions so as to limit power consumption and/or memory degradation.

Abstract: Remote control based content control that allows one or more remote control users to control content or other materials being shown or displayed through a television or other display. The control may optionally include split screen display control and/or remote control/user based content control.

Abstract: Expression vectors and yeast cells that contain a heterologous G protein-coupled receptor gene and a gene mutation that causes increased sensitivity to receptor activation or a gene mutation that permits transcriptional activation of pheromone-responsive genes without cell cycle arrest. Methods of making the yeast cells.

Abstract: The present invention is directed to expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein &agr;&bgr;&ggr; complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on the cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric G&agr; protein is expressed in conjunction with nucleotide sequences from the yeast G protein &bgr;&ggr; subunits. A second aspect of the present invention provides expression vectors and yeast cells transformed therewith encoding chimeric yeast/heterologous G protein coupled receptors.

Abstract: A first aspect of the present invention is directed to a transformed yeast cell containing a first heterologous DNA sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second heterologous DNA sequence which codes for a G protein a subunit or portions thereof fused to DNA sequences from the yeast G protein in a subunit. A second aspect of the present invention is a transformed yeast cell containing a heterologous DNA sequence which codes for a G protein coupled receptor. A third aspect of the present invention is a method of assaying compounds to determine effects on cell growth.

Abstract: A method for the identification of agents which inhibit chitin synthesis, thus exhibiting potential fungicidal and insecticidal activity, involves the incubation of test samples in neutral Saccharomyces cerevisiae cultures containing calcofluor white, a fluorochrome that causes lethal chitin hyper-polymerization in the yeast. Cultures containing samples that inhibit chitin synthesis exhibit enhanced growth because the growing fungus is rescued from the adverse effects of calcofluor white. In the practice of the invention, the test sample is added to a S. cerevisiae culture or culture area containing calcofluor white and the culture is incubated with the test sample for such time under such conditions sufficient to observe yeast cell growth inhibition in a corresponding culture or culture area containing calcofluor but no test sample.

Abstract: The present invention is directed to expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein &agr;&bgr;&ggr; complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on a cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric G&agr; protein is expressed in conjunction with nucleotide sequences from the yeast G protein &bgr;&ggr; subunits. A second aspect of the present invention provides expression vectors and yeast cells transformed therewith encoding chimeric yeast/heterologous G protein coupled receptors.

Abstract: A method for screening for potential fungicides identifies biochemical or chemical agents that inhibit the formation of the fungal sterol ergosterol by inhibiting cytochrome P450 reductase or reductase related enzymes or proteins involved in its biosynthesis. Test samples are incubated in two yeast strain cultures, and the extent of growth inhibition is compared between them. One culture is supersensitive to cytochrome P450 reductase inhibitors; the yeast strain typically contains a disrupted or deleted cytochrome P450 reductase gene. The other culture is less sensitive to the compounds, typically exhibiting enhanced permeability such as that observed in erg6 mutants. In preferred embodiments, a known cytochrome P450 reductase related electron-transfer protein inhibitor such as keto-conazole, miconazole, dinaconazole or econazole is employed as a positive control. Preferred embodiments employ solidified media, and test samples are applied to disks or wells.

Abstract: A first aspect of the present invention is directed to a transformed yeast cell containing a first heterologous DNA sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second heterologous DNA sequence which codes for a G protein .alpha. subunit or portions thereof fused to DNA sequences from the yeast G protein a subunit. A second aspect of the present invention is a transformed yeast cell containing a heterologous DNA sequence which codes for a G protein coupled receptor. A third aspect of the present invention is a method of assaying compounds to determine effects on cell growth.