Abstract: A method for isolating cell-free DNA from liquid body sample, comprising the following steps: a) Providing liquid body sample; b) Adding to said sample: a solid phase capable of binding DNA; a binding buffer comprising a detergent and a chaotropic agent; and 2-propanol, to form a binding mixture thereof; c) Washing the solid phase to remove unbound material; and d) Eluting bound cell-free DNA, wherein the majority of the eluted DNA is <400 bp.

Abstract: The present invention provides a device comprising: a housing comprising a first opening for connecting to a liquid source, a second opening for connecting with an apparatus for drawing in and expelling liquid, and a valve means comprising a filter carrier and a filter, the filter carrier supporting the filter and being movable within the housing; wherein the valve means permits liquid that is drawn into the housing from the liquid source by the apparatus to enter the apparatus by bypassing the filter but which forces liquid that is subsequently pushed from the apparatus through the second opening into the housing to be filtered through the filter.

Abstract: The present invention provides a device comprising: a housing comprising a first opening for connecting to a liquid source, a second opening for connecting with an apparatus for drawing in and expelling liquid, and a valve means comprising a filter carrier and a filter, the filter carrier supporting the filter and being movable within the housing; wherein the valve means permits liquid that is drawn into the housing from the liquid source by the apparatus to enter the apparatus by bypassing the filter but which forces liquid that is subsequently pushed from the apparatus through the second opening into the housing to be filtered through the filter.

Abstract: Disclosed are nitro-substituted squaraine reporter dyes and methods using such dyes for detecting nitroreductase enzyme activity and nitroreductase gene expression in cellular assays.

Abstract: Disclosed are methods for assay of an analyte employing acridone dyes having the structure: Z1 and Z2 represent atoms necessary to complete one ring, two fused ring, three fused ring aromatic or heteroaromatic systems, each ring having five or six atoms selected from carbon atoms and optionally no more than two atoms selected from oxygen, nitrogen and sulphur; R2, R3, R4 and R5 are selected from hydrogen, halogen, amide, hydroxyl, cyano, nitro, mono- or di-nitro-substituted benzyl, amino, mono- or di-C1-C4 alkyl-substituted amino, sulphydryl, carbonyl, carboxyl, C1-C6 alkoxy, acrylate, vinyl, styryl, aryl, heteroaryl, C1-C20 alkyl, aralkyl, sulphonate, sulphonic acid, quaternary ammonium, the groups -E-F and —(CH2—)nY; R1 is selected from hydrogen, mono- or di-nitro-substituted benzyl, C1-C20 alkyl, aralkyl, the groups -E-F and —(CH2—)nY; where E is a spacer group, F is a target bonding group; Y is selected from sulphonate, sulphate, phosphonate, phosphate, quaternary ammonium and carboxyl.

Abstract: This invention relates to the functionalized cyanine dyes and more particularly, to the synthesis of chiral 3-substituted 2,3?-dimethyl-3H-indole and its derivatives as intermediates for preparation of cyanine dyes, to methods of preparing these dyes and the dyes so prepared.

Abstract: Disclosed are new acridone dye derivatives having characteristic fluorescence lifetimes. Also disclosed are methods for labelling target biological materials employing the acridone dyes and use of the labelled materials in biological assays.

Abstract: This invention relates to the functionalized cyanine dyes and more particularly, to the synthesis of chiral 3-substituted 2,3?-dimethyl-3H-indole and its derivatives as intermediates for preparation of cyanine dyes, to methods of preparing these dyes and the dyes so prepared.

Abstract: Disclosed are nitro-substituted squaraine reporter dyes and methods using such dyes for detecting nitroreductase enzyme activity and nitroreductase gene expression in cellular assays.

Abstract: This invention relates to the functionalized cyanine dyes and more particularly, to the synthesis of chiral 3-substituted 2,3?-dymethyl-3H-indole and its derivatives as intermediates for preparation of cyanine dyes, to methods of preparing these dyes and the dyes so prepared.

Abstract: Disclosed are nitro-substituted squaraine reporter dyes and methods using such dyes for detecting nitroreductase enzyme activity and nitroreductase gene expression in cellular assays.

Abstract: Disclosed are nitro-substituted squaraine reporter dyes and methods using such dyes for detecting nitroreductase enzyme activity and nitroreductase gene expression in cellular assays.

Abstract: Disclosed are new acridone dye derivatives having characteristic fluorescence lifetimes. Also disclosed are methods for labelling target biological materials employing the acridone dyes and use of the labelled materials in biological assays.

Abstract: Disclosed are new acridone dye derivatives having characteristic fluorescence lifetimes. Also disclosed are methods for labelling target biological materials employing the acridone dyes and use of the labelled materials in biological assays.

Abstract: Novel squarate dyes are described, and adducts of these dyes with biologically significant chemical species such as nucleosides or nucleotides. The adducts have useful properties for fluorescent nucleic acid sequencing methods.