Abstract: The present disclosure describes a system for launching an activation device to a frac tree, to a frac tree including the system, and to a method of remotely launching an activation device into a frac tree. The activation device is pneumatically conveyed through a pneumatic line between a loading station located remotely from the frac tree, to a staging valve system adapted to connect to the frac tree. The staging valve system stages the activation device from a low pressure zone above the staging valve system to a high pressure zone in the frac tree.

Abstract: Certain embodiments of the disclosure can include methods, devices, and systems for locating an electronic signal sent in a large, enclosed space, including hotels and offices. The embodiments can include initiating a signal from a location within the large, enclosed structure triggered by, for example, atmospheric conditions or user input, among other triggering mechanisms. The signal can then be transmitted via low-energy, low-cost network devices placed efficiently throughout the building. Devices within the network read the message signal and efficiently continue its transmission through the network. When received by a display device, the message can be identified by its originating location and mapped against a layout of the building.

Abstract: A method may include (a) providing a cartridge including a volume of photohardenable composition in an irradiation zone; (b) selectively irradiating the volume of the photohardenable composition included in the cartridge with one or more wavelengths of excitation light to at least partially harden, polymerize, and/or cross-link the resin composition to at least partially form one or more objects in the volume; (c) removing the cartridge from the irradiation zone after selective irradiation step; and optionally repeating steps (a)-(c) with a series of one or more additional cartridges including a next volume of photohardenable composition. An assembly for printing 3D objects may include a printer having at least one excitation light source configured to solidify photohardenable resin and form one or more 3D objects in a cartridge.

Abstract: The subject matter of the present disclosure generally relates to techniques for addressing or correcting dysregulation of the trauma regulation pathway. The dysregulation may be associated with a physiological condition, such as a SARS-CoV-2 viral infection. In an embodiment, the techniques include treating dysregulation based on a renin-angiotensin pathway molecule or cell and/or a splenic pathway molecule or cell using targeted neuromodulation. In an embodiment, neuromodulation is used to regulate the immune system, e.g., as an energy-based adjuvant for a vaccine.

Abstract: The subject matter of the present disclosure generally relates to techniques for addressing or correcting dysregulation of the trauma regulation pathway. The dysregulation may be associated with a physiological condition, such as a SARS-CoV-2 viral infection. In an embodiment, the techniques include treating dysregulation based on a renin-angiotensin pathway molecule or cell and/or a splenic pathway molecule or cell using targeted neuromodulation. In an embodiment, neuromodulation is used to regulate the immune system, e.g., as an energy-based adjuvant for a vaccine.

Abstract: The present invention relates to the technical field of light sheet technology and related methods and apparatuses including light sheet technology for use in three-dimensional printing. The present invention includes methods and apparatuses including a combined light sheet including, for example, methods for improving light intensity uniformity of a light sheet including a first wavelength passing through a composition and methods of forming an object in a volume, which methods include a combined light sheet comprising a generated light sheet and reflected light sheet, and related apparatuses.

Abstract: A method is provided for generating single-stranded DNA circles from a biological sample. The method comprises the steps of: treating the biological sample with an extractant to release nucleic acids, thereby forming a sample mixture; neutralizing the extractant; denaturing the released nucleic acids to generate single-stranded nucleic acids; and contacting the single-stranded nucleic acids with a ligase that is capable of template-independent, intramolecular ligation of single-stranded DNA to generate the single-stranded DNA circles. All the steps of the method are performed without any intermediate nucleic acid isolation or nucleic acid purification. The single-stranded DNA circles may be amplified and further analyzed. Also provided is a kit which comprises compositions for carrying out the novel methods.

Abstract: Certain embodiments of the disclosure can include methods, devices, and systems for locating an electronic signal sent in a large, enclosed space, including hotels and offices. The embodiments can include initiating a signal from a location within the large, enclosed structure triggered by, for example, atmospheric conditions or user input, among other triggering mechanisms. The signal can then be transmitted via low-energy, low-cost network devices placed efficiently throughout the building. Devices within the network read the message signal and efficiently continue its transmission through the network. When received by a display device, the message can be identified by its originating location and mapped against a layout of the building.

Abstract: A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5? flanking sequence and a donor 3? flanking sequence. The donor 5? flanking sequence and the donor 3? flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.

Abstract: Certain embodiments of the disclosure can include methods, devices, and systems for locating an electronic signal sent in a large, enclosed space, including hotels and offices. The embodiments can include initiating a signal from a location within the large, enclosed structure triggered by, for example, atmospheric conditions or user input, among other triggering mechanisms. The signal can then be transmitted via low-energy, low-cost network devices placed efficiently throughout the building. Devices within the network read the message signal and efficiently continue its transmission through the network. When received by a display device, the message can be identified by its originating location and mapped against a layout of the building.

Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

Abstract: A well treatment additive includes a siloxane surfactant, a solvent and an aqueous phase. The solvent is preferably a terpene hydrocarbon. Also disclosed is a method for using the well treatment additive to form and enhance the properties of terpene solvent based additives useful for the treatment of oil and gas wells. Methods of using the novel well treatment additives include using the additives in a variety of well treatment processes including, but not limited to, acidizing operations, hydraulic fracturing operations, well remediation operations and water removal operations.

Abstract: Natural Creamers for providing whitening and indulgent texture/mouthfeel of beverage and food products are provided. The creamers have long-term stability, high whitening capacity and a pleasant mouthfeel when added to beverage and food, while being free from artificial additives. In a general embodiment, the present disclosure provides a creamer comprising added sugar, milk fat, milk proteins and optionally natural flavors and free of any artificial additives. Wherein the ratio of milk fat:milk protein is less than 5.3:1; and wherein the Lumisizer instability index of liquid creamer is less than 10.

Abstract: An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.

Abstract: A welding hood for protecting an operator's face comprising a face protective shield having a rearward side configured for receiving the operator's face and a viewing opening extending through the shield. The viewing opening is located in front of the eyes of the operator while the hood is fixed to the operator's head. An eye box is located adjacent the viewing opening and extends inwardly from the rearward side of the shield to block reflective arc flashes, sunlight, and/or shop-lighting from entering the operator's field of view during welding. The welding hood includes a light mounted to the face protective shield for shining light outward from a front side of the face protective shield, a cooling fan mounted adjacent the rearward side of the shield, and a clear protective member mounted within the eye box and adjacent the operator's eyes.

Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

Abstract: Provided are techniques for generating expression products using one or more nucleic acid concatemers that include tandem repeats of a nucleic acid sequence encoding the expression product or products. In one embodiment, different expression products may be co-expressed using a concatemer mixture of a first nucleic acid concatemer and a second nucleic acid concatemer having a predefined ratio to one another.