Abstract: The present invention describes a spatial addressing technique that uses a very high-density micro-pore array for high-throughput screening of biological interactions. The therapeutic, diagnostic, and drug-discovery implications of being able to identify, select and characterize specific protein-protein, protein-DNA and/or protein-carbohydrate interactions from heterogeneous populations of millions (to billions) of cells is discussed. Importantly, this technique possesses the screening and selection capacity of current display-based screening systems (i.e. millions-billions) but with greater efficiency and shorter time.

Abstract: The present invention describes a spatial addressing technique that uses a very high-density micro-pore array for high-throughput screening of biological interactions. The therapeutic, diagnostic and drug-discovery implications of being able to identify, select and characterize specific protein-protein, protein-DNA and/or protein-carbohydrate interactions from heterogeneous populations of millions (to billions) of cells is discussed. Importantly, this technique possesses the screening and selection capacity of current display-based screening systems (i.e., millions-billions) but with greater efficiency and shorter time.

Abstract: The present invention describes a spatial addressing technique that uses a very high-density micro-pore array for high-throughput screening of biological interactions. The therapeutic, diagnostic and drug-discovery implications of being able to identify, select and characterize specific protein-protein, protein-DNA and/or protein-carbohydrate interactions from heterogeneous populations of millions (to billions) of cells is discussed. Importantly, this technique possesses the screening and selection capacity of current display-based screening systems (i.e., millions-billions) but with greater efficiency and shorter time.

Abstract: A method of generating and isolating a recombinant high affinity anti-sialic acid antibody molecule comprises the steps of immunising a host with an immunogen comprising a conjugate of sialic acid and a carrier protein to generate an anti-sialic acid polyclonal serum, isolating a sample of RNA from the immunised avian host, and generating and screening of a library of recombinant antibody molecules from the RNA sample, and isolating a recombinant high affinity anti-sialic acid antibody molecule. The antibody molecule is selected from the group consisting of: whole antibodies; scFv fragments; and Fab fragments, and the host is Gallus domesticus. A recombinant avian antibody fragment having high binding affinity to sialic acid and obtainable by the method of the invention, and an anti-sialic acid polyclonal serum obtainable by immunising an avian host with a conjugate of sialic acid and carrier protein, are also described.

Abstract: A milk analysis apparatus for detecting mastitis in a milk sample by isolating somatic cells in the form of a pellet using centrifugal sedimentation is described. The apparatus comprises a vessel for holding the milk sample, and a centrifuge for rotating the vessel. The vessel includes an inlet for facilitating charging milk into a body portion of the vessel; and a trap for capturing somatic cells suspended in the milk sample. The somatic cells are biased towards the trap upon rotation of the vessel.

Abstract: Methods and apparatus for detecting immunochemical reactions and levels of antigens and antibodies in serum. A selected immunochemical reactant is bound to the surface of a first electrode. A second electrode is provided such that the electrodes generate a galvanic current when electrically interconnected and immersed in conductive aqueous solution. The electrodes are electrically interconnected and immersed into a conductive aqueous solution containing the complementary immunochemical reactant to the bound immunochemical reactant. The galvanic current generated by the immersed electrodes is measured as a function of time. The level of the complementary immunochemical reactant in the solution is calculated by comparing the measured current with a standard current.