Patents by Inventor Richard P. Burlingame
Richard P. Burlingame has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9862956Abstract: The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.Type: GrantFiled: January 29, 2014Date of Patent: January 9, 2018Assignee: DANISCO US INC.Inventors: Mark A. Emalfarb, Peter J. Punt, Cornelia Van Zeijl, Cornelius Van den Hondel, Jan Cornelis Verdoes, Richard P. Burlingame
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Publication number: 20170037417Abstract: The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.Type: ApplicationFiled: January 29, 2014Publication date: February 9, 2017Applicant: Dyadic International, Inc.Inventors: Mark A. EMALFARB, Peter J. PUNT, Cornelia VAN ZEIJL, Cornelius VAN DEN HONDEL, Jan Cornelis VERDOES, Richard P. BURLINGAME
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Patent number: 9303252Abstract: Provided are modified valencene synthase polypeptides and methods of using the modified valencene synthase polypeptides. Also provided are methods for producing modified terpene synthases.Type: GrantFiled: October 28, 2011Date of Patent: April 5, 2016Assignee: Evolva, Inc.Inventors: Jean Davin Amick, Grace Eunyoung Park, Bryan N. Julien, Richard P. Burlingame
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Publication number: 20150211013Abstract: The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.Type: ApplicationFiled: January 29, 2014Publication date: July 30, 2015Applicant: Dyadic International, Inc.Inventors: Mark A. EMALFARB, Peter J. PUNT, Cornelia VAN ZEIJL, Cornelius VAN DEN HONDEL, Jan Cornelis VERDOES, Richard P. BURLINGAME
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Patent number: 8916363Abstract: This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, ?-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.Type: GrantFiled: October 20, 2010Date of Patent: December 23, 2014Assignee: Dyadic International (USA), Inc.Inventors: Alexander V. Gusakov, Tatyana N. Salanovich, Alexey I. Antonov, Boris B. Ustinov, Oleg N. Okunev, Richard P. Burlingame, Mark A. Emalfarb, Marco A. Baez, Arkady P. Sinitsyn
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Patent number: 8680252Abstract: The invention provides a method for the expression and subsequent screening of DNA libraries, particularly synthetic, genomic, and cDNA libraries, in filamentous fungal hosts. In particular, the invention provides vectors, host strains, and a method for the expression and screening of complex DNA libraries, including, but not limited to, combinatory (combinatorial) libraries expressing one, two or more variable constituents and/or prepared from two or more sublibraries (e.g., for the expression and screening of immunoglobulin (including fragments and derivatives of whole immunoglobulin proteins) and other receptor or complex DNA libraries or libraries of libraries). The invention is useful for the expression and screening for a large variety of proteins and protein complexes, including human proteins. The present invention also relates to novel fungal protease sequences.Type: GrantFiled: December 10, 2007Date of Patent: March 25, 2014Assignee: Dyadic International (USA), Inc.Inventors: Mark A. Emalfarb, Peter J. Punt, Cornelia Van Zeijl, Cornelius Van Den Hondel, Jan Verdoes, Richard P. Burlingame
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Patent number: 8673618Abstract: This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, ?-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.Type: GrantFiled: October 20, 2010Date of Patent: March 18, 2014Assignee: Dyadic International (USA), Inc.Inventors: Alexander V. Gusakov, Tatyana N. Salanovich, Alexey I. Antonov, Boris B. Ustinov, Oleg N. Okunev, Richard P. Burlingame, Mark A. Emalfarb, Marco A. Baez, Arkady P. Sinitsyn
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Patent number: 8609371Abstract: The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.Type: GrantFiled: August 12, 2009Date of Patent: December 17, 2013Assignee: Allylix, Inc.Inventors: Bryan Julien, Richard P. Burlingame
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Patent number: 8486659Abstract: The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.Type: GrantFiled: August 12, 2009Date of Patent: July 16, 2013Assignee: Allylix, Inc.Inventors: Bryan Julien, Richard P. Burlingame
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Patent number: 8481286Abstract: The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.Type: GrantFiled: August 12, 2009Date of Patent: July 9, 2013Assignee: Allylix, Inc.Inventors: Bryan Julien, Richard P. Burlingame
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Publication number: 20120246767Abstract: Provided are modified valencene synthase polypeptides and methods of using the modified valencene synthase polypeptides. Also provided are methods for producing modified terpene synthases.Type: ApplicationFiled: October 28, 2011Publication date: September 27, 2012Inventors: Jean Davin Amick, Eunyoung Park, Bryan N. Julien, Richard P. Burlingame
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Publication number: 20120030839Abstract: The invention provides a method for the expression and subsequent screening of DNA libraries, particularly synthetic, genomic, and cDNA libraries, in filamentous fungal hosts. In particular, the invention provides vectors, host strains, and a method for the expression and screening of complex DNA libraries, including, but not limited to, combinatory (combinatorial) libraries expressing one, two or more variable constituents and/or prepared from two or more sublibraries (e.g., for the expression and screening of immunoglobulin (including fragments and derivatives of whole immunoglobulin proteins) and other receptor or complex DNA libraries or libraries of libraries). The invention is useful for the expression and screening for a large variety of proteins and protein complexes, including human proteins. The present invention also relates to novel fungal protease sequences.Type: ApplicationFiled: December 10, 2007Publication date: February 2, 2012Applicant: DYADIC INTERNATIONAL, INC.Inventors: Mark A. Emalfarb, Peter J. Punt, Cornelia Van Zeijl, Cornelius Van Den Hondel, Jan Verdoes, Richard P. Burlingame
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Publication number: 20110237485Abstract: The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulase. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking, color brightening, depilling and biobleaching of paper and pulp and treatment of waste streams.Type: ApplicationFiled: February 21, 2011Publication date: September 29, 2011Inventors: Mark Aaron Emalfarb, Irina Vladimirovna Solovjeva, Arie Ben-Bassat, Richard P. Burlingame, Vladimir Mikhaylovich Chernoglazov, Oleg Nicolaevich Ocounev, Philip T. Olson, Arkady Panteleimonovich Sinitsyn
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Publication number: 20110047656Abstract: This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, ?-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.Type: ApplicationFiled: October 20, 2010Publication date: February 24, 2011Inventors: ALEXANDER V. GUSAKOV, TATYANA N. SALANOVICH, ALEXEY I. ANTONOV, BORIS B. USTINOV, OLEG N. OKUNEV, RICHARD P. BURLINGAME, MARK A. EMALFARB, MARCO A. BAEZ, ARKADY P. SINITSYN
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Publication number: 20110045546Abstract: This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, ?-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.Type: ApplicationFiled: October 20, 2010Publication date: February 24, 2011Inventors: ALEXANDER V. GUSAKOV, TATYANA N. SALANOVICH, ALEXEY I. ANTONOV, BORIS B. USTINOV, OLEG N. OKUNEV, RICHARD P. BURLINGAME, MARK A. EMALFARB, MARCO A. BAEZ, ARKADY P. SINITSYN
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Patent number: 7892812Abstract: A computer-based method and apparatus for the analysis specification and support of work processes. The system is designed to support multiple interdependent decisions, at least some of which require collaboration among multiple participants (116). Work processes are modeled using an application framework (99) used to develop abstract, decision (100) process models. The decision (100) process models are used as a pattern to instantiate concrete process models that incorporate the work defined by the abstract process. The process model is then used to instantiate project models that incorporate the required work from the process. The project models are used to direct and guide the behavior of the participants (116) in the work process.Type: GrantFiled: September 30, 1997Date of Patent: February 22, 2011Assignee: Dyadic International (USA), Inc.Inventors: Mark Aaron Emalfarb, Irina Vladimirovna Solovjeva, Arie Ben-Bassat, Richard P. Burlingame, Vladimir Mikhaylovich Chernoglazov, Oleg Nicolaevich Ocounev, Philip T. Olson, Arkady Panteleimonovich Sinitsyn
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Patent number: 7883872Abstract: This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, ?-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.Type: GrantFiled: July 13, 2006Date of Patent: February 8, 2011Assignee: Dyadic International (USA), Inc.Inventors: Alexander V. Gusakov, Tatyana N. Salanovich, Alexey I. Antonov, Boris B. Ustinov, Oleg N. Okunev, Richard P. Burlingame, Mark A. Emalfarb, Marco A. Baez, Arkady P. Sinitsyn
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Publication number: 20090017520Abstract: The present invention relates to a method and materials for producing glucosamine by fermentation of a genetically modified microorganism. Included in the present invention are genetically modified microorganisms useful in the present method for producing glucosamine, as well as recombinant nucleic acid molecules and the proteins produces by such recombinant nucleic acid molecules.Type: ApplicationFiled: June 22, 2007Publication date: January 15, 2009Applicant: Arkion Life Sciences LLC d/b/a Bio-Technical ResourcesInventors: Alan Berry, Richard P. Burlingame, James R. Millis
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Publication number: 20030157595Abstract: A computer-based method and apparatus for the analysis specification and support of work processes. The system is designed to support multiple interdependent decisions, at least some of which require collaboration among multiple participants (116). Work processes are modeled using an application framework (99) used to develop abstract, decision (100) process models. The decision (100) process models are used as a pattern to instantiate concrete process models that incorporate the work defined by the abstract process. The process model is then used to instantiate project models that incorporate the required work from the process. The project models are used to direct and guide the behavior of the participants (116) in the work process.Type: ApplicationFiled: June 3, 1999Publication date: August 21, 2003Inventors: MARK AARON EMALFARB, IRINA VLADIMIROVNA SOLOVJEVA, ARIE BEN-BASSAT, RICHARD P. BURLINGAME, VLADIMIR MIKHAYLOVICH CHERNOGLAZOV, OLEG NICOLAEVICH OCOUNEV, PHILIP T. OLSON, ARKADY PANTELEIMONOVICH SINITSYN
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Publication number: 20030044939Abstract: The present invention relates to a method and materials for producing glucosamine by fermentation of a genetically modified microorganism. Included in the present invention are genetically modified microorganisms useful in the present method for producing glucsamine, as well as recombinant nucleic acid molecules and the proteins produces by such recombinant nucleic acid molecules.Type: ApplicationFiled: December 17, 2001Publication date: March 6, 2003Applicant: Arkion Life Sciences LLCInventors: Alan Berry, Richard P. Burlingame, James R. Millis